当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale.

Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale.

Abstract：

:In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography. The D. officinale polysaccharide (DOP) has a molecular weight of 8500Da. Monosaccharide composition analysis reveals that DOP is composed of mannose, glucose, and arabinose with a trace of galacturonic acid in a molar ratio of 6.2:2.3:2.1:0.1. Periodate oxidation-smith degradation and 1D and 2D NMR spectroscopy analysis suggest the predominance of mannose and glucose, and it contains a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues. Atomic force microscope shows that DOP mainly exists as rod-shaped chains, supporting high degrees of polymerization. The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Luo QL,Tang ZH,Zhang XF,Zhong YH,Yao SZ,Wang LS,Lin CW,Luo X

doi

10.1016/j.ijbiomac.2016.04.067

subject

Has Abstract

pub_date

2016-08-01 00:00:00

pages

219-27

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(16)30375-0

journal_volume

89

pub_type

杂志文章

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