当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Partial purification and MALDI-TOF MS analysis of UN1, a tumor antigen membrane glycoprotein.

Partial purification and MALDI-TOF MS analysis of UN1, a tumor antigen membrane glycoprotein.

Abstract：

:UN1 is a membrane glycoprotein that is expressed in immature human thymocytes, a subpopulation of peripheral T lymphocytes, the HPB acute lymphoblastic leukemia (ALL) T-cell line and fetal thymus. We previously reported the isolation of a monoclonal antibody (UN1 mAb) recognizing the UN1 protein that was classified as "unclustered" at the 5th and 6th International Workshop and Conference on Human Leukocyte Differentiation Antigens. UN1 was highly expressed in breast cancer tissues and was undetected in non-proliferative lesions and in normal breast tissues, indicating a role for UN1 in the development of a tumorigenic phenotype of breast cancer cells. In this study, we report a partial purification of the UN1 protein from HPB-ALL T cells by anion-exchange chromatography followed by immunoprecipitation with the UN1 mAb and MALDI-TOF MS analysis. This analysis should assist in identifying the amino acid sequence of UN1.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

de Laurentiis A,Caterino M,Orrù S,Ruoppolo M,Tuccillo F,Masullo M,Quinto I,Scala G,Pucci P,Palmieri C,Tassone P,Salvatore F,Venuta S

doi

10.1016/j.ijbiomac.2006.02.020

keywords:

subject

Has Abstract

pub_date

2006-08-15 00:00:00

pages

122-6

issue

1-3

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(06)00081-X

journal_volume

39

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Equilibrium adsorption of caffeic, chlorogenic and rosmarinic acids on cationic cross-linked starch with quaternary ammonium groups.

    abstract：:In the present study, the equilibrium adsorption of caffeic acid (CA) and its derivatives, namely, chlorogenic (CGA) and rosmarinic (RA) acids on cationic cross-linked starch (CCS) with degree of substitution of quaternary ammonium groups of 0.42 have been investigated in relation to the structure and acidity of pheno...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.12.006

    authors： Simanaviciute D,Klimaviciute R,Rutkaite R

    更新日期：2017-02-01 00:00:00

  • Molecular characteristics of an exopolysaccharide from Lactobacillus rhamnosus KF5 in solution.

    abstract：:The molecular weight and chain conformation of an exopolysaccharide (EPS), S2, from Lactobacillus rhamnosus KF5 were determined by size-exclusion chromatography combined with multi-angle laser light scattering (SEC-MALLS), dynamic light scattering (DLS), viscometry and atomic force microscopy (AFM). The weight-average...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.10.015

    authors： Shao L,Wu Z,Tian F,Zhang H,Liu Z,Chen W,Guo B

    更新日期：2015-01-01 00:00:00

  • Silencing of IL-6 and STAT3 by siRNA loaded hyaluronate-N,N,N-trimethyl chitosan nanoparticles potently reduces cancer cell progression.

    abstract：:The immunosuppressive nature of the tumor microenvironment is a critical problem that should be considered before the design of immunotherapies. Interleukin (IL)-6 and its related downstream molecules such as signal transducer and activator of transcription (STAT)3 play an important role in the cancer progression, whi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.273

    authors： Masjedi A,Ahmadi A,Atyabi F,Farhadi S,Irandoust M,Khazaei-Poul Y,Ghasemi Chaleshtari M,Edalati Fathabad M,Baghaei M,Haghnavaz N,Baradaran B,Hojjat-Farsangi M,Ghalamfarsa G,Sabz G,Hasanzadeh S,Jadidi-Niaragh F

    更新日期：2020-04-15 00:00:00

  • Characterization of films based on chitosan lactate and its blends with oxidized starch and gelatin.

    abstract：:Minimal inhibitory concentration (MIC) of chitosan lactate (CHL) was tested against bacteria and phytopathogenic fungi. Then, the structural, physicochemical and antimicrobial properties of films based on CHL, oxidized potato starch (OPS), and gelatin (GEL) were investigated. With the exception of Rhizopus nigricans, ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.03.032

    authors： Kowalczyk D,Kordowska-Wiater M,Nowak J,Baraniak B

    更新日期：2015-01-01 00:00:00

  • Purification and structural characterization of a novel antioxidant and antibacterial protein from Arca inflata.

    abstract：:A novel protein (J2-C4) with antioxidant and antibacterial activities was purified from the edible portion of Arca inflata. The purity of J2-C4 was measured to be 97.62% by RP-HPLC analysis, and the molecular weight was 20,537.0 Da by ESI-MS/MS. The isoelectric point of J2-C4 was determined to be 5.18 by IEF-PAGE. Sec...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.05.008

    authors： Wang W,Shi H,Zhu J,Li C,Song L,Yu R

    更新日期：2018-09-01 00:00:00

  • Immunostimulatory and anti-allergic potential of novel heterotrimeric lectin from seeds of Zizyphus mauritiana Lam.

    abstract：:Zizyphus mauritiana Lam. seeds (ZMS) have been used medicinally as sedative or hypnotic drugs in most of Asian countries. ZMS has significant benefits to the human health. Therefore, we have evaluated immunomodulatory effect of lectin extracted from these ZMSL in both in vitro and in vivo study. Anaphylaxis is a sever...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2021.01.027

    authors： Butle AB,Talmale SA,Jadhao VV,Patil MB,Lambat TL

    更新日期：2021-01-09 00:00:00

  • Hydroxyapatite-decorated ZrO2 for α-amylase immobilization: Toward the enhancement of enzyme stability and reusability.

    abstract：:Herein, the immobilization of α-amylase onto hydroxyapatite (HA) and hydroxyapatite-decorated ZrO2 (10%wt) (HA-ZrO2) nanocomposite were investigated. The immobilization yield was 69.7% and 84% respectively. The structural differences were characterized using X-Ray diffraction, attenuated total reflectance-Fourier tran...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.11.150

    authors： Almulaiky YQ,Khalil NM,El-Shishtawy RM,Altalhi T,Algamal Y,Aldhahri M,Al-Harbi SA,Allehyani ES,Bilal M,Mohammed MM

    更新日期：2021-01-15 00:00:00

  • Hepatoprotective effect of a polysaccharide from Crassostrea gigas on acute and chronic models of liver injury.

    abstract：:A water-soluble polysaccharide was isolated from Crassostrea gigas (CGPS-1) by hot-water extraction, isoelectric precipitation, hydrolysis and ultrafiltration without using ethanol. Based on the calibration with Dextran, CGPS-1 had a molecular weight of approximately 6.5×10(6) Da. CGPS-1 was analyzed using high-perfor...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.03.056

    authors： Shi X,Ma H,Tong C,Qu M,Jin Q,Li W

    更新日期：2015-01-01 00:00:00

  • Establishment of a hybrid drying strategy for instant cream mushroom soup based on starch retrogradation behavior.

    abstract：:Starch retrogradation during freeze drying is a contributor to an undesirable decrease in the viscosity of soup prepared from instant soup with high starch content. Monitoring starch retrogradation during drying is therefore of importance if such a process is to be optimized. Here, cream mushroom soup was used as an e...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.084

    authors： Liu W,Zhang M,Devahastin S,Wang W

    更新日期：2020-03-15 00:00:00

  • Biochemical characterization of a novel β-galactosidase from Paenibacillus barengoltzii suitable for lactose hydrolysis and galactooligosaccharides synthesis.

    abstract：:A β-galactosidase gene (PbBGal2A) was cloned from Paenibacillus barengoltzii and expressed in Escherichia coli. The in silico analysis of the deduced amino acid sequences revealed that PbBGal2A shared the highest identity of 40% with the characterized glycoside hydrolase (GH) family 2 β-galactosidase from Actinobacill...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.06.073

    authors： Liu Y,Chen Z,Jiang Z,Yan Q,Yang S

    更新日期：2017-11-01 00:00:00

  • Designing novel bioconjugates of hydroxyethyl cellulose and salicylates for potential pharmaceutical and pharmacological applications.

    abstract：:This deals with fabrication of macromolecular prodrugs (MPDs) of salicylic acid (SA) and aspirin (ASP) based on a hydrophilic cellulose ether, hydroxyethyl cellulose (HEC). Degrees of substitution (DS) of SA and ASP per HEC repeating unit (HEC-RU) were achieved ranging from 0.60 to 2.18 and 0.53 to1.50, respectively. ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.05.061

    authors： Abbas K,Amin M,Hussain MA,Sher M,Bukhari SNA,Jantan I,Edgar KJ

    更新日期：2017-10-01 00:00:00

  • Physiometabolic effects of Agave salmiana fructans evaluated in Wistar rats.

    abstract：:A prospective completely randomized experimental study was conducted using 48 animals to evaluate the physiometabolic effects of Agave salmiana fructans as a dietary supplement in healthy Wistar rats. Five fructans concentrations from 5 to 20% (w/w) and one control were used in the rats' diet and were divided into six...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.11.043

    authors： Castillo Andrade AI,Rivera Bautista C,Godínez Hernández C,Ruiz Cabrera MA,Fuentes Ahumada C,García Chávez E,Grajales Lagunes A

    更新日期：2018-03-01 00:00:00

  • Preparation and characterization of solvent-free fluids reinforced and plasticized polylactic acid fibrous membrane.

    abstract：:In this paper, the electronspun Polylactic acid (PLA)/TiO2 nanofluids (nfs) fibrous membrane with good toughness, hydrophilicity and antibacterial activities are fabricated by taking full advantages of solvent-free TiO2 nfs with amphiphilicity and ionic conductivity. The resulting PLA/TiO2 nfs fibrous membrane exhibit...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.06.027

    authors： Yu Q,Qin Y,Han M,Pan F,Han L,Yin X,Chen Z,Wang L,Wang H

    更新日期：2020-10-15 00:00:00

  • Inhibition of amyloid fibrillation of γD-crystallin model peptide by the cochineal Carmine.

    abstract：:γD-crystallin is among the most abundant γ-crystallins in the human eye lens which are essential for preserving its transparency. Aging, and environmental changes, cause crystallins to lose their native soluble structure and aggregate, resulting in the formation of cataract. Current treatment of cataract is surgical r...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.12.106

    authors： Abu-Hussien M,Viswanathan GK,Borisover L,Mimouni M,Engel H,Zayit-Soudry S,Gazit E,Segal D

    更新日期：2021-02-01 00:00:00

  • Fructose restrains fibrillogenesis in human serum albumin.

    abstract：:The endogenous deposition of protein aggregates with fibrillar morphology is known to govern the genesis of several neurodisorders. The investigation of naturally occurring small molecules which can restrict the fibrillation process can help in the design of rational therapeutics for neurodegenerative diseases. We rep...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.08.006

    authors： Pandey NK,Ghosh S,Dasgupta S

    更新日期：2013-10-01 00:00:00

  • SS-mPEG chemical modification of recombinant phospholipase C for enhanced thermal stability and catalytic efficiency.

    abstract：:PEGylation is one of the most promising and extensively studied strategies for improving the properties of proteins as well as enzymic physical and thermal stability. Phospholipase C, hydrolyzing the phospholipids offers tremendous applications in diverse fields. However, the poor thermal stability and higher cost of ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.01.134

    authors： Fang X,Wang X,Li G,Zeng J,Li J,Liu J

    更新日期：2018-05-01 00:00:00

  • Recent advances in polyhydroxyalkanoate production: Feedstocks, strains and process developments.

    abstract：:Polyhydroxyalkanoates (PHAs) have been actively studied in academia and industry for their properties comparable to petroleum-derived plastics and high biocompatibility. However, the major limitation for commercialization is their high cost. Feedstock costs, especially carbon costs, account for the majority of the fin...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.04.082

    authors： Li M,Wilkins MR

    更新日期：2020-08-01 00:00:00

  • Conformational rearrangements of G-quadruplex topology promoted by Cu(II) 12-MCCu(II)PyrAcHA-4 metallacrown.

    abstract：:Cu(II) 12-MCCu(II)PyrAcHA-4 metallacrown was studied by several spectroscopic techniques as an interacting ligand with G-quadruplex DNA structures. Investigations were performed on oligonucleotides bearing human telomeric and protooncogenic c-myc sequences in buffered solution mimicking ionic conditions in cellular en...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.11.163

    authors： Rajczak E,Juskowiak B

    更新日期：2020-08-01 00:00:00

  • Heme degradation and iron release of hemoglobin and oxidative stress of lymphocyte cells in the presence of silica nanoparticles.

    abstract：:Silica nanoparticles (SiO2 NPs) have been widely used in the medical and food sciences. However, their toxic effects against bio-macromolecules and cells are not well understood. The present study was aimed to investigate the adverse effects of fabricated SiO2 NPs on the human hemoglobin (Hb) by FTIR, CD, fluorescence...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.06.128

    authors： Azimipour S,Ghaedi S,Mehrabi Z,Ghasemzadeh SA,Heshmati M,Barikrow N,Attar F,Falahati M

    更新日期：2018-10-15 00:00:00

  • Physical properties imparted by genipin to chitosan for tissue regeneration with human stem cells: A review.

    abstract：:Genipin is a fully assessed non-cytotoxic crosslinking compound. The chitosan|genipin physical properties such as morphology, roughness, porosity, hydrophilicity, ζ-potential, surface area and surface energy exert control over cell adhesion, migration, phenotype maintenance and intracellular signaling in vitro, and ce...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2016.03.075

    authors： Muzzarelli RA,El Mehtedi M,Bottegoni C,Gigante A

    更新日期：2016-12-01 00:00:00

  • Fungicidal and anti-biofilm activities of trimethylchitosan-stabilized silver nanoparticles against Candida species in zebrafish embryos.

    abstract：:Herein, positively surface-charged silver nanoparticles (AgNPs) capped with trimethylchitosan nitrate (TMCN) were synthesized using an environmentally friendly method. Nano-sized TMCN-AgNPs (~80 nm) with high zeta potential (>30 mV) provide sufficient static repulsion to stabilize colloid AgNPs in aqueous solutions wi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.10.002

    authors： Wang SH,Chen CC,Lee CH,Chen XA,Chang TY,Cheng YC,Young JJ,Lu JJ

    更新日期：2020-01-15 00:00:00

  • Neuroprotective effects of Coptis chinensis Franch polysaccharide on amyloid-beta (Aβ)-induced toxicity in a transgenic Caenorhabditis elegans model of Alzheimer's disease (AD).

    abstract：:This study aims to investigate the neuroprotective effects of Coptis chinensis Franch polysaccharide (CCP) on Aβ1-42 transgenic CL4176 Caenorhabditis elegans, as well as its mechanism of action. The results in life span experiment showed that CCP could significantly increase the lifespan of C. elegans and the effect i...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.03.035

    authors： Li Y,Guan S,Liu C,Chen X,Zhu Y,Xie Y,Wang J,Ji X,Li L,Li Z,Zhang Y,Zeng X,Li M

    更新日期：2018-07-01 00:00:00

  • Quantification of anti-aggregation activity of UV-irradiated α-crystallin.

    abstract：:Ultraviolet radiation is a risk factor for cataractogenesis. It is believed that enhanced rates of lens opacification and cataract formation are the results of gradual loss of chaperone-like efficiency of α-crystallin upon exposure to UV light. To characterize chaperone-like activity of α-crystallin damaged by UV irra...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.10.060

    authors： Borzova VA,Markossian KA,Muranov KO,Polyansky NB,Kleymenov SY,Kurganov BI

    更新日期：2015-02-01 00:00:00

  • Analysis of Aspergillus sp. lipase immobilization for the application in organic synthesis.

    abstract：:Nowadays, for the industrial implementations, especially in the area of organic synthesis, immobilized enzymes are preferred over their soluble forms. Present study aimed to find fast, cost-efficient, and effective way of lipase immobilization for the use in organic media. Lipase from Aspergillus sp. (Resinase A 2X) w...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.11.010

    authors： Gricajeva A,Kazlauskas S,Kalėdienė L,Bendikienė V

    更新日期：2018-03-01 00:00:00

  • Antioxidant and antimicrobial carboxymethyl cellulose films containing Zataria multiflora essential oil.

    abstract：:The present study describes the physical, antioxidant, and antimicrobial properties of biodegradable films prepared by incorporating different concentrations (1, 2, and 3% v/v) of Zataria multiflora Boiss (avishan-e shirazi) essential oil (ZEO) into carboxymethyl cellulose (CMC) film. The films' tensile strength, elon...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.09.006

    authors： Dashipour A,Razavilar V,Hosseini H,Shojaee-Aliabadi S,German JB,Ghanati K,Khakpour M,Khaksar R

    更新日期：2015-01-01 00:00:00

  • Fabrication and characterization of PVA/Gum tragacanth/PCL hybrid nanofibrous scaffolds for skin substitutes.

    abstract：:In this work three dimensional biodegradable nanofiberous scaffolds containing poly(ε-caprolactone) (PCL), poly(vinyl alcohol) (PVA) and gum tragacanth (GT) were successfully fabricated through two nozzles electrospinning process. For this purpose, PVA/GT blend (Blend: B) solution (60:40wt%) was injected from one syri...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.10.042

    authors： Zarekhalili Z,Bahrami SH,Ranjbar-Mohammadi M,Milan PB

    更新日期：2017-01-01 00:00:00

  • Low molecular weight chitosan nanoparticles for CpG oligodeoxynucleotides delivery: Impact of molecular weight, degree of deacetylation, and mannosylation on intracellular uptake and cytokine induction.

    abstract：:Although synthetic CpG oligodeoxynucleotides (ODNs) have shown substantial potential as immunotherapeutic agents, their effective intracellular delivery remains challenging. In this work, nanoparticles prepared from low-molecular weight (LMW) chitosans were investigated as CpG ODN delivery systems. Chitosan samples wi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.05.048

    authors： Babii O,Wang Z,Liu G,Martinez EC,van Drunen Littel-van den Hurk S,Chen L

    更新日期：2020-09-15 00:00:00

  • WITHDRAWN: Long non-coding RNA H19 promotes angiogenesis in microvascular endothelial cells by down-regulating miR-181a.

    abstract：:This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal. ...

    journal_title：International journal of biological macromolecules

    pub_type： 撤回出版物

    doi：10.1016/j.ijbiomac.2018.08.091

    authors： Zhu A,Chu L,Ma Q,Li Y

    更新日期：2018-08-19 00:00:00

  • Toxic heavy metal cadmium removal using chitosan and polypropylene based fiber composite.

    abstract：:The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste. Polypropylene is harnessed for its importance in forming strong comp...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.07.252

    authors： Alaswad SO,Lakshmi KB,Sudha PN,Gomathi T,Arunachalam P

    更新日期：2020-12-01 00:00:00

  • Effect of triglyceride on complexation between starch and fatty acid.

    abstract：:This study aimed to reveal the effect of corn oil on formation of complex between rice starch and fatty acids, including lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0) and stearic acid (C18:0). The RVA, XRD, DSC and CLSM analysis confirmed formation of starch-fatty acid complex but the starch-trigly...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.11.072

    authors： Li X,Luo S,Hou Y,Liu Y,Hu X,Liu C

    更新日期：2020-07-15 00:00:00