Abstract:
:The molecular weight and chain conformation of an exopolysaccharide (EPS), S2, from Lactobacillus rhamnosus KF5 were determined by size-exclusion chromatography combined with multi-angle laser light scattering (SEC-MALLS), dynamic light scattering (DLS), viscometry and atomic force microscopy (AFM). The weight-average molecular weight (Mw), intrinsic viscosity [ƞ], radii of gyration (R(g)) and hydrodynamic radii (R(h)) were 7.5 × 10(5) Da, 2.331 dL/g, 44.3 nm and 29.4 nm, respectively. The conformational parameters were calculated from the above data according to the theory of dilute polymer solutions. The Mark-Houwink-Sakurada exponent α was found to be 0.687, and the value of the structure-sensitive parameter ρ (R(g)/R(h)) was 1.51. The results revealed that the EPS S2 existed as a random coil conformation in 0.1M NaNO3 aqueous solution. AFM further confirmed the random coil morphology of this molecule in aqueous solution. EPS S2 present extended chains (fibrous morphology), with circular side chains, dispersed in SDS solution.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Shao L,Wu Z,Tian F,Zhang H,Liu Z,Chen W,Guo Bdoi
10.1016/j.ijbiomac.2014.10.015subject
Has Abstractpub_date
2015-01-01 00:00:00pages
1429-34eissn
0141-8130issn
1879-0003pii
S0141-8130(14)00691-6journal_volume
72pub_type
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