Molecular characteristics of an exopolysaccharide from Lactobacillus rhamnosus KF5 in solution.

Abstract:

:The molecular weight and chain conformation of an exopolysaccharide (EPS), S2, from Lactobacillus rhamnosus KF5 were determined by size-exclusion chromatography combined with multi-angle laser light scattering (SEC-MALLS), dynamic light scattering (DLS), viscometry and atomic force microscopy (AFM). The weight-average molecular weight (Mw), intrinsic viscosity [ƞ], radii of gyration (R(g)) and hydrodynamic radii (R(h)) were 7.5 × 10(5) Da, 2.331 dL/g, 44.3 nm and 29.4 nm, respectively. The conformational parameters were calculated from the above data according to the theory of dilute polymer solutions. The Mark-Houwink-Sakurada exponent α was found to be 0.687, and the value of the structure-sensitive parameter ρ (R(g)/R(h)) was 1.51. The results revealed that the EPS S2 existed as a random coil conformation in 0.1M NaNO3 aqueous solution. AFM further confirmed the random coil morphology of this molecule in aqueous solution. EPS S2 present extended chains (fibrous morphology), with circular side chains, dispersed in SDS solution.

journal_name

Int J Biol Macromol

authors

Shao L,Wu Z,Tian F,Zhang H,Liu Z,Chen W,Guo B

doi

10.1016/j.ijbiomac.2014.10.015

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

1429-34

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(14)00691-6

journal_volume

72

pub_type

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