Purification and structural characterization of a novel antioxidant and antibacterial protein from Arca inflata.

Abstract:

:A novel protein (J2-C4) with antioxidant and antibacterial activities was purified from the edible portion of Arca inflata. The purity of J2-C4 was measured to be 97.62% by RP-HPLC analysis, and the molecular weight was 20,537.0 Da by ESI-MS/MS. The isoelectric point of J2-C4 was determined to be 5.18 by IEF-PAGE. Secondary structure analysis of J2-C4 showed that it contained 34.9% α-helix, 15.0% β-sheet, 16.3% β-turn and 34.0% random coil by CD spectroscopy. The complete amino acid sequence of J2-C4 was identified by gel electrophoresis and LC-MS/MS together with transcriptome database analysis. Based on the alignment with NCBI BLAST database, J2-C4 showed 71% homology with a sarcoplasmic calcium-binding protein isolated from Crassostrea virginica. Therefore, J2-C4 was proposed to be a new sarcoplasmic calcium-binding protein-like protein in A. inflata. In vitro antioxidant assays showed that J2-C4 exhibited favorable scavenging activities on ABTS+ (EC50 145.80 μg/mL) and DPPH (EC50 455.62 μg/mL). J2-C4 exhibited antibacterial activities against Pseudomonas aeruginosa (MIC: 375 μg/mL), Escherichia coli (MIC: 187.5 μg/mL) and Methicillin resistant Staphylococcus aureus (MIC: 750 μg/mL). The results showed that J2-C4 might be developed as a potential food additive agent.

journal_name

Int J Biol Macromol

authors

Wang W,Shi H,Zhu J,Li C,Song L,Yu R

doi

10.1016/j.ijbiomac.2018.05.008

subject

Has Abstract

pub_date

2018-09-01 00:00:00

pages

289-298

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(18)30688-3

journal_volume

116

pub_type

杂志文章
  • Chitosan stabilized camptothecin nanoemulsions: Development, evaluation and biodistribution in preclinical breast cancer animal mode.

    abstract::Clinical use of camptothecin (CPT) is hindered due to its poor water and oil solubility, active lactone ring instability and non-targeted toxicity. Recently we reported formulation of camptothecin microemulsions with increased solubility for the improved treatment of breast cancer. In this research chitosan stabilized...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.05.127

    authors: Natesan S,Sugumaran A,Ponnusamy C,Thiagarajan V,Palanichamy R,Kandasamy R

    更新日期:2017-11-01 00:00:00

  • Study of the complex coacervation mechanism between the lysing enzyme from T. harzianum and polyallylamine hydrochloride.

    abstract::Complex coacervation was achieved by mixing the lysing enzyme from T. harzianum (LYS) with polyallylamine hydrochloride (PAH). We show in this work that the study electrostatic complexes conformation can lead to the formation of dense complexes. We systematically investigated the effects of pH and the mass ratio on th...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.11.266

    authors: Bey H,Gtari W,Aschi A

    更新日期:2019-03-01 00:00:00

  • Poly(methyl methacrylate) films reinforced with coconut shell lignin fractions to enhance their UV-blocking, antioxidant and thermo-mechanical properties.

    abstract::Lignin is a high added-value product obtained from agrowastes through organosolv process to yield materials for technological applications. Here, coconut shell organosolv lignin was fractionated using green solvents (acetone and ethanol) and incorporated in poly(methyl methacrylate) (PMMA) films. The non-fractionated ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.12.043

    authors: Avelino F,de Oliveira DR,Mazzetto SE,Lomonaco D

    更新日期:2019-03-15 00:00:00

  • Dual-layered pH-sensitive alginate/chitosan/kappa-carrageenan microbeads for colon-targeted release of 5-fluorouracil.

    abstract::A new pH-sensitive drug carrier based on alginate (Alg), chitosan (Cs), and kappa-carrageenan (kC) marine biopolymers was developed, comprising a dual-layered polyelectrolyte complexes microbead structure. Fourier-transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), and scanning electron microsco...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.03.225

    authors: Sun X,Liu C,Omer AM,Yang LY,Ouyang XK

    更新日期:2019-07-01 00:00:00

  • Characterization, optimization and stability studies on Candida rugosa lipase supported on nanocellulose reinforced chitosan prepared from oil palm biomass.

    abstract::The contribution of chitosan/nanocellulose (CS-NC) to the enzymatic activity of Candida rugosa lipase covalently bound on the surface of CS-NC (CRL/CS-NC) was investigated. Cellulosic material from oil palm frond leaves (OPFL) were bleached, alkaline treated and acid hydrolyzed to obtain the purified NC and used as na...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.03.095

    authors: Elias N,Chandren S,Razak FIA,Jamalis J,Widodo N,Wahab RA

    更新日期:2018-07-15 00:00:00

  • NMR and the mobility of water in polysaccharide gels.

    abstract::An analysis of the possible modes of motion of water molecules in polysaccharide gels indicates that the interpretation of NMR results does not require the supposition of a class of 'bound water' with highly perturbed motional states. There may, however, be water molecules associated with junction zones in the gels wh...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审

    doi:10.1016/s0141-8130(97)00045-7

    authors: Belton PS

    更新日期:1997-08-01 00:00:00

  • Effects of nano-ZnO and nano-SiO2 particles on properties of PVA/xylan composite films.

    abstract::The effects of nano-ZnO and nano-SiO2 on the properties of PVA/xylan composite films were investigated in this work. Results showed that nano-ZnO and nano-SiO2 could effectively improve the mechanical strength, moisture and oxygen barrier properties and surface hydrophobic property of the composite films. The results ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.03.088

    authors: Liu X,Chen X,Ren J,Chang M,He B,Zhang C

    更新日期:2019-07-01 00:00:00

  • Influence of lignin level on release of hemicellulose-derived sugars in liquid hot water.

    abstract::Lignin layers surrounding hemicelluloses and cellulose in the plant cell walls protect them from deconstruction. This recalcitrance to sugar release is a major limitation for cost-effective industrial conversion of lignocellulosic biomass to biofuels. Many literatures had reported the contribution of lignin removal to...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.10.045

    authors: Yu Q,Zhuang X,Yuan Z,Kong X,Qi W,Wang W,Wang Q,Tan X

    更新日期:2016-01-01 00:00:00

  • Nanoconstructions based on double-stranded nucleic acids.

    abstract::We describe the formation and properties of nanoconstruction that consists of the double-stranded DNA molecules located at distance of 35-50 A in the spatial structure of particles of their cholesteric liquid-crystalline dispersions and cross-linked by artificial nanobridges. The resulting nanostructures possess the p...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2005.04.004

    authors: Yevdokimov YM,Skuridin SG,Nechipurenko YD,Zakharov MA,Salyanov VI,Kurnosov AA,Kuznetsov VD,Nikiforov VN

    更新日期:2005-07-01 00:00:00

  • Polysaccharide from Gracilaria caudata protects the human esophageal mucosal barrier: A differential topical effect and structural dependence.

    abstract::This study aimed to evaluate the in vitro protective effect of topical treatment with a native sulfated polysaccharide of G. caudata (SP-Gc), hydrolyzed (H-SP-Gc), or desulfated (D-SP-Gc) polysaccharide of Gracilaria caudata in esophageal biopsies obtained from GERD patients. Biopsies were obtained from nonerosive ref...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.02.074

    authors: Sales TMAL,Nicolau LAD,Rodrigues RLM,Nascimento RR,Paula SM,da Costa-Filho HB,Bezerra FF,Santos GRC,Barros FCN,Alencar POC,Freitas ALP,Nobre-Souza MÂ,Santos AA,Sifrim D,Soares PMG,Silva RO,Souza MHLP

    更新日期:2020-05-01 00:00:00

  • Comparative study on citric acid modified instant starches (alcoholic alkaline treated) isolated from white sorghum and corn grains.

    abstract::This study investigated the impact of citric acid modification combined with alcoholic-alkaline treatment on physicochemical, textural and rheological properties of sorghum and corn starches. Both starches were modified with citric acid (CA) using concentrations (20 and 40%) w/w and were named as SCA and CCA, respecti...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.10.143

    authors: Zehra N,Mohsin Ali T,Hasnain A

    更新日期:2020-05-01 00:00:00

  • Solubility and aggregation of soy protein isolate induced by different ionic liquids with the assistance of ultrasound.

    abstract::The aim of this study was to evaluate the effects of ionic liquids (ILs) and ultrasound on the solubility and aggregation behavior of soy protein isolate (SPI). A variety of ILs were tested. Results showed that changes in cation or anion altered the physicochemical properties of ionic liquids, which in turn influenced...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.08.031

    authors: Huang L,Zhang W,Yan D,Ma L,Ma H

    更新日期:2020-12-01 00:00:00

  • Structure and hypoglycemic activity of a novel exopolysaccharide of Cordyceps militaris.

    abstract::A novel neutral exopolysaccharide (EPS-III) was isolated from culture broth of Cordyceps militaris (C. militaris). The EPS-III was a homogeneous polysaccharide with Mw of 1.56 × 103 kDa. The yield of EPS-III from culture broth was 123.2 ± 3.1 mg/L and the sugar content was 93.32 ± 0.87%. The backbone of EPS-III was ma...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.10.207

    authors: Sun H,Yu X,Li T,Zhu Z

    更新日期:2021-01-01 00:00:00

  • Bacterial cellulose nanofibers as reinforce in edible fish myofibrillar protein nanocomposite films.

    abstract::Bacterial cellulose nanofibers (BCNF) were employed in order to improve various properties of fish myofibrillar protein (FMP) films of Silver carp. Three different levels (2, 4 and 6%, w/w) of the nanoparticles were added to the protein polymer substrate. In this regard, it became clear that 6% BCNF-containing nanocom...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.05.038

    authors: Shabanpour B,Kazemi M,Ojagh SM,Pourashouri P

    更新日期:2018-10-01 00:00:00

  • Lignin-based hydrogels: A review of preparation, properties, and application.

    abstract::Lignin as the second most abundant and the only polyaromatics-contained bio-polymer in plant has been most studied for various applications. In the past decade, the utilization of lignin for value-added materials has been extensively sought after since lignin valorization represents one of the main challenging issues ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审

    doi:10.1016/j.ijbiomac.2019.05.198

    authors: Meng Y,Lu J,Cheng Y,Li Q,Wang H

    更新日期:2019-08-15 00:00:00

  • One pot synthesis of metal ion anchored alginate-gelatin binary biocomposite for efficient Cr(VI) removal.

    abstract::Biopolymers are widely used for the removal of chromium from aqueous medium but it possesses limitations like poor sorption capacity and low stability. To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chrom...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.10.010

    authors: Gopalakannan V,Viswanathan N

    更新日期:2016-02-01 00:00:00

  • Impact of lysyl oxidase (G473A) polymorphism on diabetic foot ulcers.

    abstract::Lysyl oxidase (LOX) is an extra-cellular matrix-modifying enzyme that has been linked to cell proliferation, metastasis, angiogenesis and wound healing. This study was designed to examine the association of LOX gene polymorphism G473A, G>A, (rs1800449) located in exon 1 of the LOX gene in diabetic subjects with and wi...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.05.050

    authors: Pichu S,Sathiyamoorthy J,Vimalraj S,Viswanathan V,Chatterjee S

    更新日期:2017-10-01 00:00:00

  • Construction of a Cordyceps sinensis exopolysaccharide-conjugated selenium nanoparticles and enhancement of their antioxidant activities.

    abstract::A Cordyceps sinensis exopolysaccharide (EPS)-conjugated selenium nanoparticles (SeNPs) were successfully constructed through the reduction of SeO32-. The EPS-SeNPs were characterized in terms of formation, morphology, size, Se distribution and phase by UV-vis, FT-IR, transmission electron microscopy (TEM), dynamic lig...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.03.016

    authors: Xiao Y,Huang Q,Zheng Z,Guan H,Liu S

    更新日期:2017-06-01 00:00:00

  • NIR triggered liposome gold nanoparticles entrapping curcumin as in situ adjuvant for photothermal treatment of skin cancer.

    abstract::We report the synthesis of a biodegradable liposome gold nanoparticles for curcumin (Au-Lipos Cur NPs) delivery. This entrapped curcumin served as an in situ adjuvant for photothermal therapy. Curcumin was loaded in Au-Lipos NPs with an encapsulation efficiency of ∼70%. The gold coating enabled the NPs to specifically...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.11.163

    authors: Singh SP,Alvi SB,Pemmaraju DB,Singh AD,Manda SV,Srivastava R,Rengan AK

    更新日期:2018-04-15 00:00:00

  • Gel green fluorescence ssDNA aptasensor based on carbon nanotubes for detection of anthrax protective antigen.

    abstract::Bacillus anthracis, the causative agent of anthrax, is a harmful pathogen with potential ability as a biological weapon which persuades scientists to develop novel methods to detect anthrax from infected resources. In this study, a multi-walled carbon nanotube (MWCNTs)-based fluorescence aptasensor was fabricated to d...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.08.219

    authors: Karimi F,Dabbagh S

    更新日期:2019-11-01 00:00:00

  • Starch based biodegradable graft copolymer for the preparation of silver nanoparticles.

    abstract::The synthesis and characterization of a novel biodegradable graft copolymer based on partially hydrolyzed polymethylacrylate (PMA) grafted amylopectin (AP) was reported which was developed for the synthesis of silver nanoparticles from silver nitrate solution by facile green technique. The prepared graft copolymer was...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.07.046

    authors: Das S,Sasmal D,Pal S,Kolya H,Pandey A,Tripathy T

    更新日期:2015-11-01 00:00:00

  • Effect of microbial exopolysaccharide on wheat bran sourdough: Rheological, thermal and microstructural characteristics.

    abstract::Rheological, thermal and microstructural properties of wheat bran sourdough (WBS) containing different levels of microbial exopolysaccharide (EPS) extracted from Lactobacillus plantarum were investigated. All sourdough samples showed pseudoplastic behavior and the highest apparent viscosity was achieved at 1.5% EPS. T...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.03.149

    authors: Abedfar A,Hosseininezhad M,Rafe A

    更新日期:2020-07-01 00:00:00

  • alpha-Crystallin polymers and polymerization: the view from down under.

    abstract::Several models have been proposed for the quaternary structure of alpha-crystallin. Some suggest the subunits are arranged in concentric shells. Others propose that the subunits are in a micelle-like arrangement. However, none is able to satisfactorily account for all observations on the protein and the quaternary str...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/s0141-8130(98)00023-3

    authors: Augusteyn RC

    更新日期:1998-05-01 00:00:00

  • Recognition of active and inactive catalytic triads: A template based approach.

    abstract::It is well established that a sequence template along with the database is a powerful tool for identifying the biological function of proteins. Here, we describe a method for predicting the catalytic nature of certain proteins among the several protein structures deposited in the Protein Data Bank (PDB). For the prese...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2010.01.011

    authors: Gupta V,Prakash NA,Lakshmi V,Boopathy R,Jeyakanthan J,Velmurugan D,Sekar K

    更新日期:2010-04-01 00:00:00

  • Physicochemical and structural properties of sago starch.

    abstract::A comprehensive study was conducted to elucidate physicochemical and structural properties of sago starches. Two sago starch granules were oval in shape with an average diameter of 34.41 μm and had C-type polymorph with a crystallinity of about 28.13%. The amylose and resistant starch (RS) contents of two sago starche...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.07.310

    authors: Du C,Jiang F,Jiang W,Ge W,Du SK

    更新日期:2020-12-01 00:00:00

  • Toxic heavy metal cadmium removal using chitosan and polypropylene based fiber composite.

    abstract::The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste. Polypropylene is harnessed for its importance in forming strong comp...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.07.252

    authors: Alaswad SO,Lakshmi KB,Sudha PN,Gomathi T,Arunachalam P

    更新日期:2020-12-01 00:00:00

  • Temperature effects on alpha-crystallin structure probed by 6-bromomethyl-2-(2-furanyl)-3-hydroxychromone, an environmentally sensitive two-wavelength fluorescent dye covalently attached to the single Cys residue.

    abstract::The single Cys residue in the C-terminal domain of bovine eye lens alpha-crystallin was covalently labelled with 6-bromomethyl-2-(2-furanyl)-3-hydroxychromone. This novel SH-reactive two-band ratiometric fluorescent dye is characterized by excited state intramolecular proton transfer reaction yielding two highly emiss...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2005.07.007

    authors: Avilov SV,Bode C,Tolgyesi FG,Klymchenko AS,Fidy J,Demchenko AP

    更新日期:2005-09-28 00:00:00

  • Studies on graft copolymerization of gellan gum with N,N-dimethylacrylamide by the redox system.

    abstract::The present paper reports the graft copolymerization of N,N-dimethylacrylamide onto gellan gumby free radical polymerization using potassium peroxymonosulphate/sarbose redox system in an inert atmosphere. The reaction conditions for maximum grafting have been optimized by varying the reaction variables, including the ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2014.06.043

    authors: Pandey VS,Verma SK,Yadav M,Behari K

    更新日期:2014-09-01 00:00:00

  • Insights into the evolution of Brachyura (Crustacea: Decapoda) from mitochondrial sequences and gene order rearrangements.

    abstract::Brachyura is one of the most species rich and highly derived groups among extant crustaceans, with over 7250 known species. However, brachyuran phylogeny remains controversial and requires further study. Here, we combined 103 brachyuran mitogenomes from GenBank with 10 new mitogenomes to describe gene rearrangement pa...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.12.210

    authors: Wang Q,Wang J,Wu Q,Xu X,Wang P,Wang Z

    更新日期:2021-02-15 00:00:00

  • Enhancing the thermostability of Rhizopus chinensis lipase by rational design and MD simulations.

    abstract::To improve the thermostability of r27RCL from Rhizopus chinensis and broaden its industrial applications, we used rational design (FoldX) according to ΔΔG calculation to predict mutations. Four thermostable variants S142A, D217V, Q239F, and S250Y were screened out and then combined together to generate a quadruple-mut...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.05.243

    authors: Wang R,Wang S,Xu Y,Yu X

    更新日期:2020-10-01 00:00:00