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Some biophysical applications of motional contrast in n.m.r. microscopy.

Abstract：

:The principal advantage of the n.m.r. imaging method lies in the specific contrasts which are available. In this work we describe the use of velocity and diffusion contrast methods in biophysical applications and at microscopic spatial resolution. In the first example, involving water-protein interactions, the relationship between water self-diffusion and water concentration, as measured using pulsed gradient spin echo n.m.r., is shown. It is demonstrated that this relationship can be used to provide a water concentration image. The result is compared with the conventional proton density and transverse relaxation maps. The next example concerns the use of dynamic n.m.r. microscopy to obtain water diffusion and velocity maps for wheat grain in vivo. Finally we suggest how the method may be used in the study of polymer-water interactions in an unusual adjunct to conventional polymer self-diffusion studies.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Back PJ,Coy A,Xia Y,Callaghan PT,Diamante LM,Umbach SL

doi

10.1016/0141-8130(91)90045-v

subject

Has Abstract

pub_date

1991-06-01 00:00:00

pages

181-9

issue

3

eissn

0141-8130

issn

1879-0003

pii

0141-8130(91)90045-V

journal_volume

13

pub_type

杂志文章

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