当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Simulation-guided enzyme discovery: A new microbial source of cellobiose 2-epimerase.

Simulation-guided enzyme discovery: A new microbial source of cellobiose 2-epimerase.

Abstract：

:Cellobiose 2-epimerase (CE) is a promising industrial enzyme that can be utilized in the dairy industry. More thermostable CEs from different microorganisms are still needed for a higher lactulose productivity. This study demonstrated the feasibility to use molecular dynamics (MD) simulation as the preliminary computational filter for thermostable enzymes screening. Sequence information of eleven uncharacterized CEs were chosen to be analyzed by MD simulations. The CE from Dictyoglomus thermophilum (Dith-CE) was determined experimentally to be one of the most thermostable CEs with the highest epimerization (160 ± 6.5 U mg-1) and isomerization activities (3.52 ± 0.23 U mg-1) among all the reported CEs. This enzyme shows the highest isomerization activity at 85 °C and pH 7.0. The kinetic parameters (kcat and Km) of isomerization activity of this CE are 3.98 ± 0.3 s-1 and 235.2 ± 11.2 mM, respectively. These results suggest that the CE from Dith-CE is a promising lactulose-producing enzyme.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Xiao Y,Chen Q,Shakhnovich EI,Zhang W,Mu W

doi

10.1016/j.ijbiomac.2019.08.075

subject

Has Abstract

pub_date

2019-10-15 00:00:00

pages

1002-1008

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(19)33910-8

journal_volume

139

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Antimicrobial properties of chitosan and whey protein films applied on fresh cut turkey pieces.

    abstract：:The aim of this study was to evaluate the antimicrobial activity of edible films made from a mixture of whey proteins and chitosan, supplemented with cranberry or quince juice, and then applied on fresh cut turkey pieces. Films were used to wrap fresh cut turkey pieces inoculated with S. typhimurium, E. coli, and C. j...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.03.021

    authors： Brink I,Šipailienė A,Leskauskaitė D

    更新日期：2019-06-01 00:00:00

  • Knockdown of long non-coding RNA XIST inhibits cell viability and invasion by regulating miR-137/PXN axis in non-small cell lung cancer.

    abstract：:Long non-coding RNAs (lncRNAs) may serve as miRNA sponges to modulate the expressions of miRNA target genes. LncRNA X-inactive specific transcript (XIST) has been demonstrated to be upregulated and act as an oncogene in non-small cell lung cancer (NSCLC). However, the sponge role of XIST in NSCLC progression remains l...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.01.022

    authors： Jiang H,Zhang H,Hu X,Li W

    更新日期：2018-05-01 00:00:00

  • Characterization of a new endo-type alginate lyase from Vibrio sp. W13.

    abstract：:A gene, encoding a new alginate lyase Algb, was identified and cloned from marine bacterium Vibrio sp. W13. The recombinant alginate lyase was characterized followed by being purified on Ni-NTA Sepharose. It exhibited the highest activity (457 U/mg) at pH 8.0 and 30 °C. Interestingly, Algb possessed broader substrate ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.01.053

    authors： Zhu B,Tan H,Qin Y,Xu Q,Du Y,Yin H

    更新日期：2015-04-01 00:00:00

  • Structural characterisation and immunomodulatory activity of a neutral polysaccharide from Sambucus adnata Wall.

    abstract：:A neutral polysaccharide, SPW-2, was purified from the leaves of Sambucus adnata Wall. using water extraction and alcohol precipitation, Sevage deproteination, ion exchange chromatography and gel filtration chromatography. This molecule had an average molecular weight of 7040 Da and was composed of arabinose, xylose, ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.11.021

    authors： Yuan L,Zhong ZC,Liu Y

    更新日期：2020-07-01 00:00:00

  • Highly porous of hydroxyethyl cellulose biocomposite scaffolds for tissue engineering.

    abstract：:This study is focusing to develop a porous biocompatible scaffold using hydroxyethyl cellulose (HEC) and poly (vinyl alcohol) (PVA) with improved cellular adhesion profiles and stability. The combination of HEC and PVA were synthesized using freeze-drying technique and characterized using SEM, ATR-FTIR, TGA, DSC, and ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.10.156

    authors： Zulkifli FH,Hussain FSJ,Harun WSW,Yusoff MM

    更新日期：2019-02-01 00:00:00

  • Effect of a modified thymine on the structure and stability of [d(TGGGT)]4 quadruplex.

    abstract：:Telomeric guanine-rich sequence can adopt quadruplex structures that are important for their biological role in chromosomal stabilisation. G quartets are characterised by the cyclic hydrogen bonding of four guanine bases in a coplanar arrangement and their stability is ion-dependent. In this work we compare the stabil...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/s0141-8130(02)00073-9

    authors： Petraccone L,Erra E,Nasti L,Galeone A,Randazzo A,Mayol L,Barone G,Giancola C

    更新日期：2003-01-15 00:00:00

  • Antioxidant activity of glycoprotein purified from Undaria pinnatifida measured by an in vitro digestion model.

    abstract：:The present study was performed to investigate the chemical composition and antioxidant activity of glycoprotein purified from Undaria pinnatifida Harvey (UPGP). On SDS-PAGE, UPGP migrated as a single band with a molecular weight of approximately 10 kDa and confirmed by staining with Schiff's reagent as glycoprotein. ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.09.009

    authors： Rafiquzzaman SM,Kim EY,Kim YR,Nam TJ,Kong IS

    更新日期：2013-11-01 00:00:00

  • Both beta sheet breaker and alpha helix forming pentapeptide inhibits protein fibrillation: Implication for the treatment of amyloid disorders.

    abstract：:For the first time, the effect of two novel designed pentapeptides on amyloid growth of human insulin using combined biophysical, microscopic, cell viability and computational approaches. Collective experimental data from ThT, ANS, and TEM demonstrate that in spite of having contrasting features, both peptides can eff...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.11.222

    authors： Siddiqi MK,Majid N,Alam P,Malik S,Alam A,Rajan S,Ajmal MR,Khan RH

    更新日期：2020-01-15 00:00:00

  • E.p.r. studies of photolysis of nitrosyl haemoglobin at low temperatures.

    abstract：:Photolysis of HbNO has been studied from 6.2 K to 15.5 K by electron spin resonance during and after continuous illumination. Non-exponential kinetics of both dissociation and reassociation of NO was observed. The prolonged illumination separates the fast and slow ligands. This picture is consistent with NO tunnelling...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/0141-8130(90)90083-m

    authors： Linhares MP,el-Jaick LJ,Bemski G,Wajnberg E

    更新日期：1990-02-01 00:00:00

  • The first mitochondrial genome for Phaudidae (Lepidoptera) with phylogenetic analyses of Zygaenoidea.

    abstract：:Phauda flammans Walker belongs to Phaudidae (Lepidoptera), which is a holometabolous and leaf-eating pest that harms trees. So far, there is no mitochondrial (mt) genome reported of Phaudidae. Herein, we sequenced and annotated the complete mt genome of P. flammans representing the first mt genome of Phaudidae and pre...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.307

    authors： Zhang R,Li J,Geng S,Yang J,Zhang X,An Y,Li C,Cui H,Li X,Wang Y

    更新日期：2020-04-15 00:00:00

  • Superhydrophobic cotton fabrics coated by chitosan and titanium dioxide nanoparticles with enhanced antibacterial and UV-protecting properties.

    abstract：:Superhydrophobic cotton fabrics were fabricated using chitosan/titanium dioxide (TiO2) nanocomposites. Morphology results revealed that the fabric's surface was utterly coated by the nanoparticles leading to the formation of a highly packed nano-scale structure in the case of superhydrophobic coating. X-ray photoelect...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.12.220

    authors： Raeisi M,Kazerouni Y,Mohammadi A,Hashemi M,Hejazi I,Seyfi J,Khonakdar HA,Davachi SM

    更新日期：2021-01-06 00:00:00

  • Synthesis of chitosan based molecularly imprinted polymer for pipette-tip solid phase extraction of Rhodamine B from chili powder samples.

    abstract：:The purpose work is devoted to design of a simple, one-pot and green approach for the synthesis of molecularly imprinted polymer to construct a selective sorbent for pipette-tip solid phase extraction of Rhodamine B from chili powder samples and its subsequence separation and quantification by high performance liquid ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.07.196

    authors： Bagheri AR,Ghaedi M

    更新日期：2019-10-15 00:00:00

  • Elongation pattern and fine structure of the sheaths formed by Thiothrix nivea and Thiothrix fructosivorans.

    abstract：:Thiothrix strains are filamentous sulfur-oxidizing bacteria common in activated sludge. Some of the members, including Thiothrix nivea and T. fructosivorans, are known to form a microtubular sheath that covers a line of cells. The sheaths are assemblages of [→4)-β-d-GlcN-(1→4)-β-d-Glc-(1→]n modified with unusual deoxy...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.11.025

    authors： Kawasaki Y,Endo T,Fujiwara A,Kondo K,Katahira M,Nittami T,Sato M,Takeda M

    更新日期：2017-02-01 00:00:00

  • Protective effects of Ulva pertusa polysaccharide and polysaccharide‑iron (III) complex on cyclophosphamide induced immunosuppression in mice.

    abstract：:A water-soluble polysaccharide was isolated from marine green algae Ulva pertusa and then chelated with iron to prepare the polysaccharide‑iron (III) complex. The immunomodulatory activities of sulfated polysaccharide and polysaccharide‑iron (III) complex were investigated through a mice immune-deficiency model. Cyclo...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.04.101

    authors： Gao X,Qu H,Gao Z,Zeng D,Wang J,Baranenko D,Li Y,Lu W

    更新日期：2019-07-15 00:00:00

  • The role of Cys271 in conformational changes of arginine kinase.

    abstract：:Arginine kinase (AK), a crucial enzyme in energy metabolism, buffers cellular ATP levels by catalyzing the reversible phosphoryl transfer between ATP and arginine. To better understand the role of Cys271 in conformational changes of AK from greasyback shrimp (Metapenaeus ensis), we replaced the residue with serine and...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2011.04.002

    authors： Liu N,Wang JS,Wang WD,Pan JC

    更新日期：2011-07-01 00:00:00

  • Biosynthesis and statistical optimization of polyhydroxyalkanoate (PHA) produced by Bacillus cereus VIT-SSR1 and fabrication of biopolymer films for sustained drug release.

    abstract：:Polyhydroxyalkanoates are a fascinating group of biological polymers, which have attracted extensive attention. An effectual PHB accumulating Bacillus cereus strain VIT-SSR1 was isolated from industrial effluent contaminated site. Plackett-Burman design was used to screen the critical factors affecting PHA production....

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.05.163

    authors： Evangeline S,Sridharan TB

    更新日期：2019-08-15 00:00:00

  • Physicochemical and drug release characteristics of acetylated starches of five Lagenaria siceraria cultivars.

    abstract：:Modified starches play a crucial role in the pharmaceutical industries in controlling the drug release at a pre-determined rate. The effect of acetylation on the physicochemical and drug release characteristics of the starches from five different Indian L. siceraria cultivars was investigated. Starches isolated from t...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.10.007

    authors： Kulkarni SD,Sinha BN,Kumar KJ

    更新日期：2015-01-01 00:00:00

  • In vitro and in vivo antioxidant activity of a fructan from the roots of Arctium lappa L.

    abstract：:To explore new antioxidant resource from food, a water-soluble polysaccharide (ALP1) was extracted and purified from the roots of Arctium lappa L. (A. lappa L.) through hot water extraction followed by ethanol precipitation, ion-exchange chromatography and gel filtration. The antioxidant activity of ALP1 was then eval...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.01.062

    authors： Liu W,Wang J,Zhang Z,Xu J,Xie Z,Slavin M,Gao X

    更新日期：2014-04-01 00:00:00

  • The role of pH, temperature and nucleation in the formation of cholesteric liquid crystal spherulites from chitin and chitosan.

    abstract：:The alpha chitin and chitosan used in the experiments came from crab shell waste (Sigma). This was treated to form a colloidal suspension of chitin or chitosan crystallites. The electrostatic 'charge coat' surrounding the chitin was then manipulated. This was achieved by alteration of the pH of the chitin or chitosan ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/s0141-8130(98)00002-6

    authors： Murray SB,Neville AC

    更新日期：1998-04-01 00:00:00

  • Structure and cytotoxic activity of ulvan extracted from green seaweed Ulva lactuca.

    abstract：:The structure of an ulvan obtained by water extraction from green seaweed Ulva lactuca was elucidated by using IR, NMR, SEC-MALL and ESIMS methods. The ulvan was also evaluated for its cytotoxic effects on three human cancer cell lines. The results showed that the ulvan was composed of rhamnose, galactose, xylose, man...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.09.040

    authors： Thanh TT,Quach TM,Nguyen TN,Vu Luong D,Bui ML,Tran TT

    更新日期：2016-12-01 00:00:00

  • Effects of electron beam irradiation on the properties of waxy maize starch and its films.

    abstract：:Waxy maize starch was irradiated under different doses of radiation (2-30 kGy), and starch physicochemical properties were analysed. Films were subsequently produced from native and irradiated waxy maize starches and their properties were tested. The starch molecular weight markedly decreased with increasing irradiati...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.287

    authors： Zhou X,Ye X,He J,Wang R,Jin Z

    更新日期：2020-05-15 00:00:00

  • Development of an enzyme-enhancer system to improve laccase biological activities.

    abstract：:The present investigation reports an in-vitro study using combination of laccase and an enhancer capable of inhibiting the growth of pathogenic microorganisms, preventing biofilm formation, and whitening teeth. Laccase-cinnamic acid system remarkably inhibited the growth of Aggregatibacter actinomycetemcomitans, Candi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2021.01.068

    authors： Mojtabavi S,Khoshayand MR,Fazeli MR,Faramarzi MA,Samadi N

    更新日期：2021-01-15 00:00:00

  • Relationship between the molecular structure of duckweed starch and its in vitro enzymatic degradation kinetics.

    abstract：:Starch molecular structural effects in duckweed (Lemna minor and Landoltia punctata) controlling in vitro enzymatic degradation kinetics was studied. The molecular size distributions of fully-branched starches and the chain length distributions (CLDs) of enzymatically debranched duckweed starches were obtained using s...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.07.206

    authors： de Souza Moretti MM,Yu W,Zou W,Franco CML,Albertin LL,Schenk PM,Gilbert RG

    更新日期：2019-10-15 00:00:00

  • An investigation on biosorption of nitrate from water by chitosan based organic-inorganic hybrid biocomposites.

    abstract：:In this study, three types of crosslinked organic-inorganic hybrid biocomposites, such as chitosan/bentonite, chitosan/titanium oxide, and chitosan/alumina (ChBT, ChTi, and ChAl respectively) were prepared and utilized for the removal of nitrate from water by batch biosorption experiments. Effects of crosslinker dose,...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.01.066

    authors： Golie WM,Upadhyayula S

    更新日期：2017-04-01 00:00:00

  • Chromium (III) coordination capacity of chitosan.

    abstract：:Polycationic chitosan has a strong coordination to heavy metal ions due to its multifunctional hydroxyl and amino groups. However, due to the fast and facile dissolution of chitosan in acidic medium, it is difficult to measure the exact adsorption amount or coordination capacity accurately. In this work, a simple meth...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.203

    authors： Ganglo C,Rui J,Zhu Q,Shan J,Wang Z,Su F,Liu D,Xu J,Guo M,Qian J

    更新日期：2020-04-01 00:00:00

  • The impact of various exogenous type starch on the structural properties and dispersion stability of autoclaved lotus seed starch.

    abstract：:In order to investigate the effects of exogenous V-type starch on the structural properties and dispersion stability of lotus seed starch after autoclave treatment, the crystal structure, molecular structure, and dispersion stability were analyzed and discussed, as well as compared with exogenous A-type and B-type sta...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2021.01.175

    authors： Zheng Y,Ou Y,Zhang C,Zhang Y,Zheng B,Zeng S,Zeng H

    更新日期：2021-01-29 00:00:00

  • Synthesis and characterization of some novel antimicrobial thiosemicarbazone O-carboxymethyl chitosan derivatives.

    abstract：:Three novel thiosemicarbazone O-carboxymethyl chitosan derivatives were obtained via a condensation reaction of thiosemicarbazide O-carboxymethyl chitosan with o-hydroxybenzaldehyde, p-methoxybenzaldehyde, and p- chlorobenzaldehyde respectively. Their structures were characterized by elemental analysis, FTIR, (13)C NM...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.10.044

    authors： Mohamed NA,Mohamed RR,Seoudi RS

    更新日期：2014-02-01 00:00:00

  • In situ preparation of glycoconjugate hollow microspheres mimics the extracellular matrix via interfacial polymerization.

    abstract：:A novel chitin-graft-polyleucine microsphere with hollow construction was prepared via ring-opening polymerization initiated with chitin based on interfacial protocol. The FT-IR and (1)H NMR analysis demonstrated the conjugation with regulated graft length. The study provided a facile one-step route to obtain microsph...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2008.02.007

    authors： Wang J,Liu C,Chi P

    更新日期：2008-06-01 00:00:00

  • Physicochemical characterization of polysaccharide from the leaf of Dendrobium officinale and effect on LPS induced damage in GES-1 cell.

    abstract：:The polysaccharide was first successfully isolated from the leaf of Dendrobium officinale by hot water extraction and alcohol precipitation and further purified using DEAE-52 and Sephadex G-100 chromatography. The structure of LDOP-1 was characterized by HPLC, GPC, and FT-IR and NMR spectroscopy, and its protective ef...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.026

    authors： Yang K,Lu T,Zhan L,Zhou C,Zhang N,Lei S,Wang Y,Yang J,Yan M,Lv G,Chen S

    更新日期：2020-04-15 00:00:00

  • Surface modification of microcrystalline cellulose: Physicochemical characterization and applications in the Stabilization of Pickering emulsions.

    abstract：:The phenomenon of polymer adsorption was applied to modify the surface of microcrystalline cellulose (MCC). The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs. The average particle size of unmodified MCC was about 14.208 ± 0.064 μm, and it was...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.04.051

    authors： Ahsan HM,Zhang X,Li Y,Li B,Liu S

    更新日期：2019-07-01 00:00:00