Effect of polyethylene glycols on the alkaline-induced molten globule intermediate of bovine serum albumin.

Abstract:

:In the present study, the formation of one molten globule-like unfolding intermediate of bovine serum albumin (BSA) at pH 11.2 has been established with the help of circular dichroism (CD) spectra, fluorescence spectroscopy and 'phase diagram' approach. Additionally, we have shown the conformational changes occurring in the pH 11.2 intermediate of BSA when it was exposed to different molecular weight polyethylene glycols (PEGs) at varying concentrations. When the pH 11.2 intermediate of BSA was treated by PEG 400 there was induction of secondary and non-native tertiary contacts. In case of PEG 8000 and PEG 20,000, the loss in secondary as well as tertiary structure was observed. PEG 8000 and 20,000 altered the conformation of the pH 11.2 intermediate and resulted in its transition to another intermediate state in which the hydrophobic patches were inaccessible.

journal_name

Int J Biol Macromol

authors

Qu P,Wang Y,Wu G,Lu Z,Xu M

doi

10.1016/j.ijbiomac.2012.04.021

subject

Has Abstract

pub_date

2012-07-01 00:00:00

pages

97-104

issue

1-2

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(12)00152-3

journal_volume

51

pub_type

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