Abstract：
:The 129-residue lysozyme has been shown to form amyloid fibrils in vitro. While methylene blue (MB), a compound in the phenothiazinium family, has been shown to dissemble tau fibril formation, its anti-fibrillogenic effect has not been thoroughly characterized in other proteins/peptides. This study examines the effects of MB on the in vitro fibrillogenesis of lysozyme at pH 2.0 and 55 °C. Our results demonstrated that, upon 7-day incubation, the plateau ThT fluorescence of the sample was found to be ~8.69% or ~2.98% of the control when the molar ratio of lysozyme to MB was at 1:1.11 or 1:3.33, respectively, indicating that the inhibitory potency of MB against lysozyme fibrillogenesis is positively correlated with its concentration. We also found that MB is able to destabilize the preformed lysozyme fibrils. Moreover, molecular docking and molecular dynamics simulations results revealed that MB's mechanism of fibril formation inhibition may be triggered by binding with lysozyme's aggregation-prone region. Results reported here provide solid support for MB's effect on amyloid fibrillogenesis. We believe the additional insights gained herein may pave way to the discovery of other small molecules that may have similar action toward amyloid fibril formation and its associated diseases.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
How SC,Cheng YH,Lo CH,Lai JT,Lin TH,Bednarikova Z,Antosova A,Gazova Z,Wu JW,Wang SSdoi
10.1016/j.ijbiomac.2018.08.038subject
Has Abstractpub_date
2018-11-01 00:00:00pages
1059-1067eissn
0141-8130issn
1879-0003pii
S0141-8130(18)32680-1journal_volume
119pub_type
杂志文章abstract：BACKGROUND:Alginate is one of the main extracellular polymeric substances (EPS) in biofilms of Cystic Fibrosis (CF) patients suffering from pulmonary infections. Gentamicin sulfate (GS) can strongly bind to alginate resulting in loss of pharmacological activity; however neither the mechanism nor its repercussion is ful...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.10.025
更新日期：2019-01-01 00:00:00
abstract：:Chemical and structure properties of Ethanol lignin (EL), alkali lignin (AL), milled wood lignin (MWL) and cellulase enzymatic lignin (CEL) extracted from acorn shell of Quercus variabilis Bl. was studied by GPC, TG, SEM, FT-IR, 13C NMR, DEPT-135 NMR, 2D-HSQC and functional groups measurement. Results indicated that A...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.09.099
更新日期：2018-02-01 00:00:00
abstract：:The three-dimensional structure of Dioclea reflexa seed lectin (DrfL) was studied in detail by a combination of X-ray crystallography, molecular docking and molecular dynamics. DrfL was purified by affinity chromatography using Sephadex G-50 matrix. Its primary structure was obtained by mass spectrometry, and crystals...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.01.092
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abstract：:Rice hull polysaccharides (RHPS) have been reported to activate innate immunity in mice. This study investigated the effects of RHPS on natural killer (NK) cell-mediated cytotoxicity in vitro and the possible underlying anticancer mechanisms in vivo. The results showed that sustained exposure to RHPS increased NK-92MI...
journal_title：International journal of biological macromolecules
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doi：10.1016/j.ijbiomac.2018.11.200
更新日期：2019-03-01 00:00:00
abstract：:Results of small angle laser light scattering experiments carried out on polystyrene latex beads coated with lysate of Plasmodium falciparum (Pf) antigen (Ag) and human seropositive sera (10:1 ratio) are reported for various NaCl concentrations (0-300 mM). The protein coated beads showed time-dependent coagulation. Th...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(00)00113-6
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abstract：:Three strains of Spirulina platensis isolated from different locations showed capability of synthesizing poly(3-hydroxybutyrate) [P(3HB)] under nitrogen-starved conditions with a maximum accumulation of up to 10 wt.% of the cell dry weight (CDW) under mixotrophic culture conditions. Intracellular degradation (mobiliza...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2005.05.002
更新日期：2005-08-01 00:00:00
abstract：:Treatment strategy for various bone fracture and defects the researchers are focusing to develop a new carrier for delivering the drug into injured area with controlled and sustained manner using biomaterials with dynamic architecture orientation. Ceramic materials are resembled with bone compositional architecture an...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2017.11.146
更新日期：2018-04-15 00:00:00
abstract：:Soybean straw cannot be efficiently degraded and utilized by ruminants due to the complex cross-linked structure among cellulose, hemicellulose, and lignin in its cell wall. Xylanase can degrade the xylan component of hemicellulose, destroy the xylan-lignin matrix and, consequently, would theoretically improve the hyd...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.02.187
更新日期：2020-05-15 00:00:00
abstract：:The potential gastroprotection of polysaccharides (SP) isolated from maté (Ilex paraguariensis) leaves of different growth stages, under different sunlight conditions and of processing methods were evaluated. The SP consist of type I arabinogalactan (AG1) containing a (1→4)-linked β-Galp chain, with substituents of ar...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.04.038
更新日期：2013-08-01 00:00:00
abstract：:The interaction of nanoparticles (NPs) with proteins is a topic of high relevance for the medical application of nanomaterials. In this study, a comprehensive investigation was performed to clarify the binding mechanism, adsorption isotherms and kinetics of the interaction between silver nanoparticles (AgNPs) and tryp...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.03.172
更新日期：2018-07-15 00:00:00
abstract：:For the majority of the last century, commercial routes to regenerated cellulose fibres have coped with the difficulties of making a good cellulose solution by using an easy to dissolve derivative (e.g. xanthate in the case of viscose rayon) or complex (e.g. cuprammonium rayon). For the purposes of this paper, advance...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/0141-8130(96)81836-8
更新日期：1995-12-01 00:00:00
abstract：:Porous scaffolds composed of alginate (AG) and chitosan (CS) were fabricated by combining the formation of polyelectrolyte complex (PEC) with freeze-drying. The AG scaffold was used as a framework with uniformly distributed and interconnected pore structure. The chitosan or chitosan/hydroxyapatite (CS/HA) composite so...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2009.11.004
更新日期：2010-03-01 00:00:00
abstract：:The aim of this study was to determine the antitumor and antiangiogenic effects of the Schisandra chinensis polysaccharides (SCP) in selected renal cell carcinoma (RCC) cells and evaluate its potential mechanism of action. In vitro, endothelial growth factor (VEGF) secretion by Caki-1 was blockaded in response to SCP ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.02.025
更新日期：2014-05-01 00:00:00
abstract：:Myeloid-derived suppressor cells (MDSCs) accumulate in tumor-bearing hosts and play a major role in tumor-induced immunosuppression. The potent modulatory effects of polysaccharides on the innate and adaptive immune system stimulate antitumor responses. In this study, a polysaccharide with an apparent molecular weight...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.04.153
更新日期：2018-08-01 00:00:00
abstract：:Three different varieties of oat grains, Sabzaar, SKO-20, SKO-90 maintained at the moisture level of 12% were γ-irradiated at four different dosage levels - 5, 10, 15, and 20kGy. Starch was extracted and its physicochemical, structural, thermal, and antioxidant properties were studied. Amongst the physico-chemical pro...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.05.092
更新日期：2017-11-01 00:00:00
abstract：:Many polysaccharides have biological activities and have been investigated for their antitumor effects. In this study, we investigated the anti-tumor activity and anti-virus activity of SNP-the water-soluble polysaccharides extracted from Sipunculus nudus on Hepg2.2.15. Flow cytometry analysis demonstrated that SNP in...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.03.022
更新日期：2016-06-01 00:00:00
abstract：:To investigate polysaccharide structure from Lonicera japonica, and study its effects on behavior of pancreatic cells, a homogenous polysaccharide, LJ-02-1, was extracted and purified from flowers of L. japonica by DEAE-cellulose and Sephacryl S-200HR column. The molecular weight was estimated to be 54kDa. Monosacchar...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.03.025
更新日期：2016-07-01 00:00:00
abstract：:The process of recombinant protein production in E. coli system is often hampered by the formation of insoluble aggregates. Human Dihydrofolate reductase (hDHFR), an enzyme involved in the synthesis of purine, thymidilate and several other amino acids like glycine, methionine and serine is highly aggregation prone. It...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.05.143
更新日期：2017-10-01 00:00:00
abstract：:Hydrogels based on pectin and cellulose nanocrystals (CNC) were used in our study to nucleation and growth of hydroxyapatite (HAp) by the biomimetic method. In this study, we evaluated the direct impact of the different percentages of CNC on pectin hydrogel and the influence of HAp obtained through two methods. CNC we...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.12.012
更新日期：2021-01-15 00:00:00
abstract：:We report the synthesis of a series of ionic liquids (ILs) from various ions having different kosmotropicity including dihydrogen phosphate (H(2)PO(4)(-)), hydrogen sulfate (HSO(4)(-)) and acetate (CH(3)COO(-)) as anions and chaotropic cation such as trialkylammonium cation. To characterize the biomolecular interactio...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.04.017
更新日期：2012-07-01 00:00:00
abstract：:Surface acylation of microcrystalline cellulose (MCC) was performed using rice bran oil (RBO). The resultant acylated MCC (RAMCC) exhibited reduced polarity as compared with MCC. Attenuated Total Reflection (ATR)- Fourier transform infrared (FTIR) spectroscopy confirmed hydrophobic MCC modification. RAMCC and MCC were...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.06.076
更新日期：2018-10-15 00:00:00
abstract：:An analysis of the possible modes of motion of water molecules in polysaccharide gels indicates that the interpretation of NMR results does not require the supposition of a class of 'bound water' with highly perturbed motional states. There may, however, be water molecules associated with junction zones in the gels wh...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/s0141-8130(97)00045-7
更新日期：1997-08-01 00:00:00
abstract：:In this study, highly porous N/S doped magnetic carbon aerogel (N/S-MCA) was successfully fabricated using sugarcane bagasse based cellulose and used for the removal of bisphenol‑A (BPA) from aqueous solution. The fabricated N/S-MCA nanocomposite derived from cellulose was characterized by various analytical technique...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.04.004
更新日期：2019-07-01 00:00:00
abstract：:Water-soluble polysaccharides were extracted from the caps and stipes of thirteen boletus mushrooms representing five different species collected in Southwest China. Investigations of their structures and antioxidant activities allowed an evaluation of structure-function relationships. The polysaccharides were compose...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.02.084
更新日期：2018-07-01 00:00:00
abstract：:Chitosan-based hydrogel nanoparticles provide a higher brain concentration of methotrexate (MTX) following IV administration in comparison with the drug's simple solution. The present study investigates the mechanism of this phenomenon, focusing on the possible role of P-gp. A previously developed MTX containing chito...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.12.093
更新日期：2019-03-15 00:00:00
abstract：:β/γ-Crystallins, the predominant structural proteins in vertebrate lens with lifelong stability to maintain lens transparency, share a high similarity in their primary sequences and tertiary structures. Four conserved Trp residues have been shown to be important to γ-crystallin structure, stability and protection agai...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.05.109
更新日期：2017-10-01 00:00:00
abstract：:This study investigates the properties of films made from fish myofibrillar protein (FMP) incorporated with combinations of catechin-Kradon extract (C/K) (20%, w/w) at different ratios. The experimental films were compared to a polyvinyl chloride (PVC). The thickness and the transparency of the films were in the range...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.10.011
更新日期：2018-02-01 00:00:00
abstract：:Lung cancer, associated with the up-regulated epidermal growth factor receptor (EGFR) led to the development of EGFR targeted anticancer therapeutics. The biopolymeric nanoparticles form an outstanding system for the targeted delivery of therapeutic agents. The present work evaluated the in vitro effects of chitosan c...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.06.009
更新日期：2014-08-01 00:00:00
abstract：:The morphological analysis of novel quantum dot-hyaluronic acid (QDot-HA) conjugates was carried out with a transmission electron microscope (TEM). Adipic acid dihydrazide-modified HA (HA-ADH) was synthesized and conjugated to quantum dots (QDots) having carboxyl terminal ligands which were activated with 1-ethyl-3-(3...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2007.09.002
更新日期：2008-01-01 00:00:00
abstract：:Microalgae have recently been recognized as a valuable resource for various applications. Phaeodactylum tricornutum is a diatom that lives in marine water and has an unusually high content of lipids. In this study, we added P. tricornutum into a gelatin dope solution to examine the effect of this diatom using electros...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.11.004
更新日期：2014-02-01 00:00:00
