Abstract:
:Three strains of Spirulina platensis isolated from different locations showed capability of synthesizing poly(3-hydroxybutyrate) [P(3HB)] under nitrogen-starved conditions with a maximum accumulation of up to 10 wt.% of the cell dry weight (CDW) under mixotrophic culture conditions. Intracellular degradation (mobilization) of P(3HB) granules by S. platensis was initiated by the restoration of nitrogen source. This mobilization process was affected by both illumination and culture pH. The mobilization of P(3HB) was better under illumination (80% degradation) than in dark conditions (40% degradation) over a period of 4 days. Alkaline conditions (pH 10-11) were optimal for both biosynthesis and mobilization of P(3HB) at which 90% of the accumulated P(3HB) was mobilized. Transmission electron microscopy (TEM) revealed that the mobilization of P(3HB) involved changes in granule quantity and morphology. The P(3HB) granules became irregular in shape and the boundary region was less defined. In contrast to bacteria, in S. platensis the intracellular mobilization of P(3HB) seems to be faster than the biosynthesis process. This is because in cyanobacteria chlorosis delays the P(3HB) accumulation process.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Jau MH,Yew SP,Toh PS,Chong AS,Chu WL,Phang SM,Najimudin N,Sudesh Kdoi
10.1016/j.ijbiomac.2005.05.002keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
144-51issue
3eissn
0141-8130issn
1879-0003pii
S0141-8130(05)00084-Xjournal_volume
36pub_type
杂志文章abstract::Creatine kinase (CK) is a key enzyme involved in intracellular energy homeostasis. The distinct tissue distribution of muscle CK (MMCK) and brain CK (BBCK) implies that they function under conditions facing dissimilar environmental stresses. We found that MMCK and BBCK were significantly different in their stability a...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2010.03.025
更新日期:2010-07-01 00:00:00
abstract::Collagen is mostly found in fibrous tissues such as tendons, ligaments and skin. Collagen makes up approximately 30% of the proteins within the body. These are tough and strong structures found all over the body: in bones, tendons and ligaments. Collagen being the most abundant protein provides tensile strength via ce...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
doi:10.1016/j.ijbiomac.2015.07.001
更新日期:2015-09-01 00:00:00
abstract::Thermococcus gammatolerans is anaerobic euryarchaeon which grows optimally at 88 °C and its genome encodes a family B DNA polymerase (Tga PolB). Herein, we cloned the gene of Tga PolB, expressed and purified the gene product, and characterized the enzyme biochemically. The recombinant Tga PolB can efficiently synthesi...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.03.204
更新日期:2020-08-01 00:00:00
abstract::Lignin was extracted from oil palm empty fruit bunches under four different conditions. The lignin samples were characterized and employed in the green synthesis of silver nanoparticles. Two-dimensional HSQC NMR analysis showed that lignins extracted under more aggressive conditions (3.5% acid, 60 min) exhibited less ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.11.104
更新日期:2021-01-15 00:00:00
abstract::Lanthanide nanoparticles and nanorods especially their biocompatible oxide forms like cerium oxide nanoparticles (CNPs) with therapeutic applications are used to cure neurological oxidative stresses. Thus it tempts to study their biocompatible aspects by interactions with several biologically significant molecules. In...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.03.067
更新日期:2020-08-01 00:00:00
abstract::Phauda flammans Walker belongs to Phaudidae (Lepidoptera), which is a holometabolous and leaf-eating pest that harms trees. So far, there is no mitochondrial (mt) genome reported of Phaudidae. Herein, we sequenced and annotated the complete mt genome of P. flammans representing the first mt genome of Phaudidae and pre...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.01.307
更新日期:2020-04-15 00:00:00
abstract::Three polysaccharide fractions (TPS1, TPS2 and TPS3) with different molecular weights were obtained using ultra filtration membranes from crude tea polysaccharide (CTPS) extracted from abandoned lower grade tea leaves. Each fraction contained different contents of neutral sugar, uronic acid, protein, and total polyphe...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2011.12.028
更新日期:2012-04-01 00:00:00
abstract::This study provides the optimum preparation parameters for functional chitosan silver nanocomposite (CSN) films with promising antibacterial efficacy though prepared with very low silver nitrate (AgNO3) concentration. Chitosan nano‑silver composites were fabricated by In-situ chemical method utilizing the reducing abi...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.05.057
更新日期:2018-09-01 00:00:00
abstract::Tigernut starch is an underutilized food resource. In this study, pullulanase (PUL) hydrolysis was used to change its physiochemical properties for different food applications. The content of low digestible fractions, resistant starch and slow digestible starch, in PUL modified tigernut starch significantly increased ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.04.068
更新日期:2017-09-01 00:00:00
abstract::Limited proteolysis of streptokinase (Sk) by trypsin and thermolysin was performed under various incubation conditions and analysed by polyacrylamide gel electrophoresis. Several fragments (Sk1, Tr27, Tr17, Th26, and Th16) were isolated and characterized further. The N-terminal sequences of Tr27, Tr17, Th26, Th16 and ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/0141-8130(92)90007-u
更新日期:1992-04-01 00:00:00
abstract::The morphological analysis of novel quantum dot-hyaluronic acid (QDot-HA) conjugates was carried out with a transmission electron microscope (TEM). Adipic acid dihydrazide-modified HA (HA-ADH) was synthesized and conjugated to quantum dots (QDots) having carboxyl terminal ligands which were activated with 1-ethyl-3-(3...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2007.09.002
更新日期:2008-01-01 00:00:00
abstract::Prior treatment of groundnut leaves with glucan isolated from a biocontrol agent, Acremonium obclavatum, protected against the rust disease. Glucan treated leaves showed increased levels of chitinase and β-1,3-glucanase in the apoplastic fluid. An increase in endogenous levels of salicylic acid also was observed in tr...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.04.190
更新日期:2018-09-01 00:00:00
abstract::The physicochemical properties of 4-α-glucanotransferase (4αGTase)-modified rice flours were examined by measuring the molecular weight distribution, moisture sorption isotherm, and melting enthalpy of ice crystals. The results obtained by measuring the moisture sorption isotherm and melting enthalpy of ice crystals r...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2013.04.032
更新日期:2013-09-01 00:00:00
abstract::Methods for reducing and preventing postoperative abdominal adhesions have been researched for decades; however, despite these efforts, the formation of postoperative peritoneal adhesions is continuously reported. Adhesions cause serious complications such as postoperative pain, intestinal obstruction, and infertility...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.02.026
更新日期:2016-06-01 00:00:00
abstract::The difference of enzymatic degradation behavior between Poly (butylene succinate-co-diethylene glycol succinate) (PBS-co-DEGS) and Poly (butylene succinate-co-butylene diglycolic acid) (PBS-co-BDGA) was studied in a Tetrahydrofuran (THF)/toluene mixed system by Novozym 435 (N435, immobilized Candida Antarctica lipase...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.06.062
更新日期:2018-10-15 00:00:00
abstract::Albumin and hyaluronic acid are biodegradable, endogenous substances with potential use as drug carriers. These properties combined with the physical structure of the gel can lead to the formation of biologically active materials with application in medicine. This work investigated the gelation process of albumin solu...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.06.063
更新日期:2020-10-15 00:00:00
abstract::The objective of this work is to research impacts of oxidized (OCS), acetylated (ACS) and cross-linked (CLCS) corn starch on gelatinization properties of rice flour and their gel structure during storage at 4 °C for 1 and 7 days. OCS led to significant increase of pasting temperature and solubility in blend flour but ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.03.002
更新日期:2020-06-15 00:00:00
abstract::Time-dependent intermolecular sulphydryl-disulphide interchange involving beta-lactoglobulin adsorbed at the oil-water interface in n-tetradecane-in-water emulsions (10 wt% oil, 0.5 wt% protein, pH 7.0) has been investigated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). While only monome...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/0141-8130(91)90006-g
更新日期:1991-02-01 00:00:00
abstract::The supramolecular interaction between salazosulfapyridine (SASP) and hydroxypropyl-β-cyclodextrin (HP-β-CD), as well as the influence of HP-β-CD on SASP's binding to human serum albumin (HSA), were investigated. Phase-solubility studies indicate that the HP-β-CD/SASP inclusion complex was formed at a 1:1 host-guest s...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.07.033
更新日期:2016-11-01 00:00:00
abstract::Human α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase (hACMSD) is a zinc containing amidohydrolase which is a vital enzyme of the kynurenine pathway in tryptophan metabolism. It prevents the accumulation of quinolinic acid (QA) and helps in the maintenance of basal Trp-niacin ratio. To assess the structure base...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.12.005
更新日期:2018-03-01 00:00:00
abstract::We report the synthesis of a biodegradable liposome gold nanoparticles for curcumin (Au-Lipos Cur NPs) delivery. This entrapped curcumin served as an in situ adjuvant for photothermal therapy. Curcumin was loaded in Au-Lipos NPs with an encapsulation efficiency of ∼70%. The gold coating enabled the NPs to specifically...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.11.163
更新日期:2018-04-15 00:00:00
abstract::Wound dressings require good antiseptic properties, mechanical strength and, more trustably, natural material ingredients. Antimicrobial properties of cerium ions and chitosan are known and alginate based wound dressings are commercially available. In this study, the advantages of these materials were combined and alg...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.07.144
更新日期:2017-12-01 00:00:00
abstract::2,2',4,4'-tetrahydroxybenzophenone (Uvinul D50), a sunscreen used in cosmetics, has two effects in the melanin biosynthesis pathway. On the one hand, it acts a weak inhibitor of tyrosinase and on the other, it accelerates the conversion of dopachrome to melanin. Uvinul D50 was seen to behave as a weak competitive inhi...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.02.032
更新日期:2017-05-01 00:00:00
abstract::The present study investigates the hypolipidemic effects of sulphated polysaccharide obtained from Codium fragile (CFSP) in induced obese rats (HFD). The results showed an increase in body weight of HFD rats by 21.56% as compared to control normal rats. Moreover, serum lipase activity underwent an increase which led t...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.04.017
更新日期:2017-09-01 00:00:00
abstract::Levans produced by several Zymomonas mobilis strains were classified by their viscosity average molecular weight and tested against sarcoma 180. Measurements of the samples' polydispersity were carried out to characterise the molecular weight distribution. The antitumour activities of levan samples were plotted agains...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/s0141-8130(00)00125-2
更新日期:2000-07-12 00:00:00
abstract::Purple acid phosphatase (PAP) family genes play a crucial role in the phosphorus (P) foraging and recycling. There are 25 putative Jatropha curcas PAP genes (JcrPAP) were identified and classified into three groups based on their molecular weights. Subcellular localization prediction indicated that most of the JcrPAPs...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.11.027
更新日期:2019-02-15 00:00:00
abstract::A putative cDNA of α-l-rhamnosidase was PCR-cloned from Aspergillus niger JMU-TS528 and further extracellular over-expressed in Pichia pastoris GS115. The activity of the recombinant α-l-rhamnosidase r-Rha1 was 711.9U/mL, eightfold higher than the native α-l-rhamnosidase from A. niger JMU-TS528. r-Rha1 is a N-glycosyl...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.12.093
更新日期:2016-04-01 00:00:00
abstract::Hydrogen-Deuterium exchange kinetics of β-lactoglobulin and β-lactoglobulin (-)-epicatechin solutions has been investigated through the analysis of the amide I absorption band at 1650cm-1 in the FTIR spectrum. H-D substitution in NH amides and residues of the protein results in a slight red-shift and in intensity chan...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.06.028
更新日期:2017-11-01 00:00:00
abstract::Sulfated polysaccharides were shown to benefit metabolic syndrome (MS) and gut microbiota, but the contribution of sulfate group remains unclear. In this study, sulfated polysaccharides from pacific abalone (AGSP) and its desulfated product (D-AGSP) were prepared, and the contribution of sulfate group was analyzed via...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.010
更新日期:2020-12-01 00:00:00
abstract::The main purpose of this manuscript is to describe a phenomenon in which vacuum degassing a reconstituted freeze-dried fusion protein etanercept formulation caused a significant amount of protein sub-visible particles (SbVP). Physical stability of etanercept was monitored by micro-flow imaging (MFI), dynamic light sca...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2014.02.001
更新日期:2014-05-01 00:00:00