Abstract:
:To investigate polysaccharide structure from Lonicera japonica, and study its effects on behavior of pancreatic cells, a homogenous polysaccharide, LJ-02-1, was extracted and purified from flowers of L. japonica by DEAE-cellulose and Sephacryl S-200HR column. The molecular weight was estimated to be 54kDa. Monosaccharide composition was determined to be rhamnose, galacturonic acid, galactose and arabinose in the molar ratio of 10.77:7.88:15.45:65.89 by analyzing the PMP derivatives of the monosaccharides from 2M trifluoracetic acid hydrolysis via HPLC. Based on methylation analysis, partial acid hydrolysis, and NMR spectra, the polysaccharide was elucidated to be a rhamnogalacturonan backbone and substituted partly at C-4 of rhamnose. The branches were determined to be T- and 1,4,6-linked β-d-Galp, T- and 1,5-linked α-l-Araf. The polysaccharide might inhibit BxPC-3 and PANC-1 pancreatic cancer cells growth at the concentration of 1mg/mL with inhibitory ratio of 66.7% and 52.1%, respectively.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Lin L,Wang P,Du Z,Wang W,Cong Q,Zheng C,Jin C,Ding K,Shao Cdoi
10.1016/j.ijbiomac.2016.03.025subject
Has Abstractpub_date
2016-07-01 00:00:00pages
130-7eissn
0141-8130issn
1879-0003pii
S0141-8130(16)30249-5journal_volume
88pub_type
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