当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > A new water-soluble polysaccharide from Echinops pungens Trautv roots. Part I. Isolation, purification, characterization and antioxidant activity.

A new water-soluble polysaccharide from Echinops pungens Trautv roots. Part I. Isolation, purification, characterization and antioxidant activity.

Abstract：

:EPPS, a new heteropolysaccharide from Echinops pungens roots, was extracted by warm water (70 °C) and fortuitously purified using column chromatographies on DEAE-cellulose A52 and Sephadex G-100. Total carbohydrate content, specific optical rotation and molecular mass of EPPS were 98.3%, -88.2 and 65.7 kDa, respectively. GLC analysis revealed that EPPS was a neutral polysaccharide and contained Gal, Glc, Man, Xyl, and Ara at ratios of 0.96: 5.23:1.00:2.06:1.04. Multiple chemical and instrumental analysis methods such as methylation, periodate oxidation and Smith degradation, partial acid hydrolysis, GLC-MS, FT-IR and NMR (1H and 13C) were employed to elucidate the structure of EPPS and their results showed that its backbone consists of →6)-β-D-Glcp-(1 → residues, with branches attached to O-4 (~38%) by →4)-β-L-Arap-(1→, →4)-β-D-Manp-(1→, →4)-β-D-Xylp-(1→, β-D-Xylp-(1 → and α-D-Galp-(1 → residues. DPPH radical scavenging ability of EPPS was lower than ascorbic acid at identical concentrations and its EC50 value was 1.91 mg/ml.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Hoseiniyan Benvidi SM,Jahanbin K

doi

10.1016/j.ijbiomac.2020.06.128

subject

Has Abstract

pub_date

2020-10-15 00:00:00

pages

909-916

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(20)33566-2

journal_volume

161

pub_type

杂志文章

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