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Characterization and antioxidant activities of polysaccharides from the leaves of Lilium lancifolium Thunb.

Abstract：

:In this study, LLP-1, LLP-2, and LLP-3 three novel polysaccharide fractions were purified from the leaves of Lilium lancifolium, and their physicochemical characterizations and antioxidant properties were investigated by chemical methods, high performance gel permeation chromatography (HPGPC), high performance liquid chromatography (HPLC), fourier transform infrared spectrometry (FT-IR), scanning electron microscopy (SEM), and DPPH radical assay, hydroxyl radical assay, superoxide radical assay and ferrous ion chelating assay, respectively. Results showed that LLP-1, LLP-2, and LLP-3 had low protein and uronic acid contents, meanwhile, their weight-average molecular weight were estimated to be 2.25×106, 2.02×106, and 2.08×106Da, respectively. Mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose glucose and galactose were the major monosaccaherides components presenting in the polysaccharide fractions. Three polysaccharide fractions were not observed triple-helical conformation, while possessed variant surface structure. In addition, three polysaccharide fractions all exhibited significantly scavenging activities against free radical and chelate Fe2+in vitro.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Xu Z,Wang H,Wang B,Fu L,Yuan M,Liu J,Zhou L,Ding C

doi

10.1016/j.ijbiomac.2016.07.028

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

148-155

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(16)30787-5

journal_volume

92

pub_type

杂志文章

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