Biochemical characterization and molecular docking of cloned xylanase gene from Bacillus subtilis RTS expressed in E. coli.

Abstract:

:This study employed mesophilic Bacillus subtilis RTS strain isolated from soil with high xylanolytic activity. A 642 bp (xyn) xylanase gene (GenBank accession number MT677937) was extracted from Bacillus subtilis RTS and cloned in Escherichia coli BL21 cells using pET21c expression system. The cloned gene belongs to glycoside hydrolase family 11 with protein size of approximately 23 KDa. The recombinant xylanase showed optimal enzyme activity at 60 °C and at pH 6.5. Thermostability of recombinant xylanase was observed between the temperature range of 30-60 °C. Xylanase also remained stable in different concentration of various organic solvents (ethanol, butanol). This might be due to the formation of protein/organic solvent interface which prevents stripping of essential water molecules from enzyme, thus enzyme conformation and activity remained stable. Finally, the molecular docking analysis through AutoDock Vina showed the involvement of Tyr 108, Arg140 and Pro144 in protein-ligand interaction, which stabilizes this complex. The observed stability of recombinant xylanase at higher temperature and in the presence of organic solvent (ethanol, butanol) suggested possible application of this enzyme in biofuel and other industrial applications.

journal_name

Int J Biol Macromol

authors

Saleem A,Waris S,Ahmed T,Tabassum R

doi

10.1016/j.ijbiomac.2020.12.001

subject

Has Abstract

pub_date

2021-01-31 00:00:00

pages

310-321

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(20)35159-X

journal_volume

168

pub_type

杂志文章
  • Biochemistry of fish stomach chitinase.

    abstract::Fish are reported to exhibit chitinase activity in the stomach. Analyses of fish stomach chitinases have shown that these enzymes have the physiological function of degrading chitinous substances ingested as diets. Osteichthyes, a group that includes most of the fishes, have several chitinases in their stomachs. From ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审

    doi:10.1016/j.ijbiomac.2017.03.118

    authors: Ikeda M,Kakizaki H,Matsumiya M

    更新日期:2017-11-01 00:00:00

  • Ferroptosis - An iron- and disorder-dependent programmed cell death.

    abstract::Programmed cell death (PCD) is an integral component of both developmental and pathological features of an organism. Recently, ferroptosis, a new form of PCD that is dependent on reactive oxygen species and iron, has been described. As with apoptosis, necroptosis, and autophagy, ferroptosis is associated with a large ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审

    doi:10.1016/j.ijbiomac.2019.05.221

    authors: Djulbegovic MB,Uversky VN

    更新日期:2019-08-15 00:00:00

  • Inhibition of amyloid fibril formation and cytotoxicity by a chemical analog of Curcumin as a stable inhibitor.

    abstract::Clinical application of curcumin for Alzheimer's disease treatment is severely limited with regard to its poor bioavailability, high rate of metabolism, and instability under neutral condition. In the current study, we designed three compounds in which the diketone moiety of curcumin was replaced by cyclohexanone. In ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.04.038

    authors: Ramshini H,mohammad-zadeh M,Ebrahim-Habibi A

    更新日期:2015-01-01 00:00:00

  • Poly(methyl methacrylate) films reinforced with coconut shell lignin fractions to enhance their UV-blocking, antioxidant and thermo-mechanical properties.

    abstract::Lignin is a high added-value product obtained from agrowastes through organosolv process to yield materials for technological applications. Here, coconut shell organosolv lignin was fractionated using green solvents (acetone and ethanol) and incorporated in poly(methyl methacrylate) (PMMA) films. The non-fractionated ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.12.043

    authors: Avelino F,de Oliveira DR,Mazzetto SE,Lomonaco D

    更新日期:2019-03-15 00:00:00

  • Biochemical and structural characterization of Cryptosporidium parvum Lactate dehydrogenase.

    abstract::The protozoan parasite Cryptosporidium parvum causes waterborne diseases worldwide. There is no effective therapy for C. parvum infection. The parasite depends mainly on glycolysis for energy production. Lactate dehydrogenase is a major regulator of glycolysis. This paper describes the biochemical characterization of ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2014.12.019

    authors: Cook WJ,Senkovich O,Hernandez A,Speed H,Chattopadhyay D

    更新日期:2015-03-01 00:00:00

  • Mechanism of co-aggregation in a protein mixture with small additives.

    abstract::Co-aggregation plays an important role in processing protein-rich food materials under heterogeneous conditions. The main cause of co-aggregation is an electrostatic attraction between oppositely charged molecules. This study investigated thermal aggregation of β-lactoglobulin (BLG) (pI=5.1) and lysozyme (LYZ) (pI=10....

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.10.004

    authors: Oki S,Iwashita K,Kimura M,Kano H,Shiraki K

    更新日期:2018-02-01 00:00:00

  • Adsorption of copper(II) ions by a chitosan-oxalate complex biosorbent.

    abstract::Oxalic acid, an effective metal-chelating ligand, is abundant in natural resources. In this study, a chitosan-oxalate complex biosorbent (COCB) was prepared by an iontropic cross-linking method. The COCB beads were characterized by Fourier transform infrared (FT-IR) spectra, X-ray diffraction (XRD) and thermogravimetr...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2014.08.006

    authors: Mi FL,Wu SJ,Lin FM

    更新日期:2015-01-01 00:00:00

  • Anti-obesity effect and protection of liver-kidney functions by Codium fragile sulphated polysaccharide on high fat diet induced obese rats.

    abstract::The present study investigates the hypolipidemic effects of sulphated polysaccharide obtained from Codium fragile (CFSP) in induced obese rats (HFD). The results showed an increase in body weight of HFD rats by 21.56% as compared to control normal rats. Moreover, serum lipase activity underwent an increase which led t...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.04.017

    authors: Kolsi RBA,Jardak N,Hajkacem F,Chaaben R,Jribi I,Feki AE,Rebai T,Jamoussi K,Fki L,Belghith H,Belghith K

    更新日期:2017-09-01 00:00:00

  • Hydrophobic surface functionalization of lignocellulosic jute fabrics by enzymatic grafting of octadecylamine.

    abstract::Enzymatic grafting of synthetic molecules onto lignins provides a mild and eco-friendly alternative for the functionalization of lignocellulosic materials. In this study, laccase-mediated grafting of octadecylamine (OA) onto lignin-rich jute fabrics was investigated for enhancing the surface hydrophobicity. First, the...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.05.007

    authors: Dong A,Fan X,Wang Q,Yu Y,Cavaco-Paulo A

    更新日期:2015-08-01 00:00:00

  • Activated hyaluronic acid/collagen composite hydrogel with tunable physical properties and improved biological properties.

    abstract::Self-crosslinkable and injectable hydrogels were fabricated with collagen type I (Col I) and N-hydroxy sulfosuccinimide activated hyaluronic acid (HA-sNHS) at physiological conditions without any initiators or crosslinkers. The physical properties of hydrogels, such as gelation time, swelling property, degradation pro...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.07.319

    authors: Gao Y,Liu Q,Kong W,Wang J,He L,Guo L,Lin H,Fan H,Fan Y,Zhang X

    更新日期:2020-12-01 00:00:00

  • Factors influencing the morphology and adsorption performance of cellulose nanocrystal/iron oxide nanorod composites for the removal of arsenic during water treatment.

    abstract::The removal of arsenic from reservoirs is a matter of great concern in many parts of the world. Since adsorption is one of the most effective methods for treating arsenic-containing media, iron oxide nanorods were prepared using cellulose nanocrystals (CNs) as a template to remove this harmful metal from aqueous solut...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.11.182

    authors: Dong F,Xu X,Shaghaleh H,Guo J,Guo L,Qian Y,Liu H,Wang S

    更新日期:2020-08-01 00:00:00

  • Multiparticulate based thermosensitive intra-pocket forming implants for better treatment of bacterial infections in periodontitis.

    abstract::Considering alarming projections in the prevalence of periodontitis, following study was undertaken to develop chitosan-vanillin crosslinked microspheres loaded in-situ gel (MLIG) implants containing ornidazole and doxycycline hyclate for the treatment of pocket infections. Firstly, microspheres were formulated and op...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.04.179

    authors: Yadav SK,Khan G,Bansal M,Thokala S,Bonde GV,Upadhyay M,Mishra B

    更新日期:2018-09-01 00:00:00

  • Fabrication of a novel and ultrasensitive label-free electrochemical aptasensor for detection of biomarker prostate specific antigen.

    abstract::In this study, a novel and efficient aptasensor based on immobilization of thiol terminated prostate specific antigen (PSA) binding DNA aptamer onto Au nanoparticles/fullerene C60-chitosan-ionic liquid/multiwalled carbon nanotubes/screen printed carbon electrode has been fabricated for ultrasensitive aptasensing of bi...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.01.012

    authors: Jalalvand AR

    更新日期:2019-04-01 00:00:00

  • In vitro and in vivo antioxidant activity of a fructan from the roots of Arctium lappa L.

    abstract::To explore new antioxidant resource from food, a water-soluble polysaccharide (ALP1) was extracted and purified from the roots of Arctium lappa L. (A. lappa L.) through hot water extraction followed by ethanol precipitation, ion-exchange chromatography and gel filtration. The antioxidant activity of ALP1 was then eval...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2014.01.062

    authors: Liu W,Wang J,Zhang Z,Xu J,Xie Z,Slavin M,Gao X

    更新日期:2014-04-01 00:00:00

  • Effects of osmolytes on human brain-type creatine kinase folding in dilute solutions and crowding systems.

    abstract::The effects of osmolytes on the unfolding and refolding process of recombinant human brain-type creatine kinase (rHBCK) were comparatively, quantitatively studied in dilute solutions and macromolecular crowding systems (simulated by 100 g/L polyethylene glycol 2000), respectively. The results showed that the osmolytes...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2012.07.026

    authors: Fan YQ,Lee J,Oh S,Liu HJ,Li C,Luan YS,Yang JM,Zhou HM,Lü ZR,Wang YL

    更新日期:2012-12-01 00:00:00

  • The transition of polymers into a network of polymers alters per se the water activity.

    abstract::We have found that polyacrylamide gels cross-linked with bisacrylamide induce a larger macromolecular osmotic pressure than the non cross-linked gels. This means also that in the cross-linked gels hydration water is held more tightly to the polymer, i.e., more work is needed to remove water, than in the non cross-link...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2004.11.002

    authors: Trombetta G,Di Bona C,Grazi E

    更新日期:2005-03-01 00:00:00

  • Glycation of hemoglobin leads to the immunogenicity as a result of neo-epitope generation.

    abstract::Non-enzymatic glycation occurs rapidly which ultimately leads to the formation of advanced glycation endproducts (AGEs). These AGEs have shown to associated with the development of many diseases such as diabetes-mellitus. This study is focused on immunological characterization of glycated-Hb induced by d-ribose. Here,...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.11.063

    authors: Siddiqui Z,Faisal M,Alatar AA,Ahmad S

    更新日期:2019-02-15 00:00:00

  • Pectin-microfibrillated cellulose microgel: Effects on survival of lactic acid bacteria in a simulated gastrointestinal tract.

    abstract::Using high pressure microfluidization, we prepared micro-fibrillated soybean cellulose (MFSC) and analyzed its morphology and structure. MFSC was then incorporated into low-methoxyl pectin (PC) to coat lactic acid bacteria (LAB) by ionotropic gelation, and the effects of PC-MFSC microgel on LAB survival in a simulated...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.04.161

    authors: Chen B,Lin X,Lin X,Li W,Zheng B,He Z

    更新日期:2020-05-06 00:00:00

  • Hyaluronic acid-coated ZIF-8 for the treatment of pneumonia caused by methicillin-resistant Staphylococcus aureus.

    abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common causes of hospital infection. Here, we showed that hyaluronic acid modified organic metal framework material ZIF-8 could be a Trojan horse of vancomycin (Van) for effective treatment of MRSA infections. The Van-loaded nanoparticles were readi...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.03.187

    authors: Liu Y,Li Z,Zou S,Lu C,Xiao Y,Bai H,Zhang X,Mu H,Zhang X,Duan J

    更新日期:2020-07-15 00:00:00

  • Effect of testosterone and its aliphatic and aromatic dimers on DNA morphology.

    abstract::Conjugation of DNA with testosterone and it aliphatic dimer (alip) and aromatic dimer (arom) was investigated in aqueous solution at pH 7.4. Multiple spectroscopic methods, transmission electron microscopy (TEM) and molecular modeling were used to characterize steroid-DNA binding and DNA morphology. Spectroscopic anal...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.09.090

    authors: Chanphai P,Agudelo D,Vesper AR,Bérubé G,Tajmir-Riahi HA

    更新日期:2017-02-01 00:00:00

  • Design and development of Albizia stipulata gum based controlled-release matrix tablets in cancer therapeutics.

    abstract::The present study deals with the development of natural macromolecule gum Albizia stipulata (AS) based novel pharmaceutical excipient for the controlled-release of paracetamol (PC). Central composite design (CCD) two-factor, five-level was used for the optimization of independent variables AS gum and compression force...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.08.006

    authors: T VS,Henry LJ,Narra K,Lalduhsanga P,Kandasamy R

    更新日期:2016-11-01 00:00:00

  • Silk as templates for hydroxyapatite biomineralization: A comparative study of Bombyx mori and Antheraea pernyi silkworm silks.

    abstract::Silk is extensively investigated in bone tissue engineering due to its extraordinary mechanical properties and ability to regulate biomineralization. Protein templates regulate biomineralization process through chemical interaction with ions. However, the effect of structural differences in silk fibroin on biominerali...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.08.142

    authors: Zhang H,You R,Yan K,Lu Z,Fan Q,Li X,Wang D

    更新日期:2020-12-01 00:00:00

  • Comparative study on citric acid modified instant starches (alcoholic alkaline treated) isolated from white sorghum and corn grains.

    abstract::This study investigated the impact of citric acid modification combined with alcoholic-alkaline treatment on physicochemical, textural and rheological properties of sorghum and corn starches. Both starches were modified with citric acid (CA) using concentrations (20 and 40%) w/w and were named as SCA and CCA, respecti...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.10.143

    authors: Zehra N,Mohsin Ali T,Hasnain A

    更新日期:2020-05-01 00:00:00

  • Evaluation of chitosan hydrochloride-alginate as enteric micro-probiotic-carrier with dual protective barriers.

    abstract::In this study, the cells-free and cells-loaded chitosan hydrochloride-alginate (CHC-Alg) microcapsules were firstly fabricated with polyelectrolyte complexes via an orifice-polymerization method. Scanning electron microscope images showed that the CHC-Alg microcapsules had a typical shell-core structure and the model ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.09.034

    authors: Wu QX,Xu X,Xie Q,Tong WY,Chen Y

    更新日期:2016-12-01 00:00:00

  • Anti-inflammatory activity of polysaccharides from Phellinus linteus by regulating the NF-κB translocation in LPS-stimulated RAW264.7 macrophages.

    abstract::Inflammatory response remains one of the most common and serious complications of disease. In order to profound understanding relationship between Phellinus linteus polysaccharides (TCM) and inflammation, inflammatory cell model was constructed by LPS acting RAW264.7 cell line. The results showed that TCM could decrea...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.02.023

    authors: Xie Z,Wang Y,Huang J,Qian N,Shen G,Chen L

    更新日期:2019-05-15 00:00:00

  • Self-healing, sensitive and antifreezing biomass nanocomposite hydrogels based on hydroxypropyl guar gum and application in flexible sensors.

    abstract::In this manuscript, self-healing, highly flexible hydrogels based on hydroxypropyl guar gum (HPG) were prepared by adding dopamine-coated reduced-graphene oxide (rGO@PDA). The multiple reversible dynamic networks were simultaneously constructed by boron-oxygen and metal-ligand interactions. These multiple networks sig...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.11.134

    authors: Sun Z,Wang L,Jiang X,Bai L,Wang W,Chen H,Yang L,Yang H,Wei D

    更新日期:2020-07-15 00:00:00

  • Polysaccharide from Gracilaria caudata protects the human esophageal mucosal barrier: A differential topical effect and structural dependence.

    abstract::This study aimed to evaluate the in vitro protective effect of topical treatment with a native sulfated polysaccharide of G. caudata (SP-Gc), hydrolyzed (H-SP-Gc), or desulfated (D-SP-Gc) polysaccharide of Gracilaria caudata in esophageal biopsies obtained from GERD patients. Biopsies were obtained from nonerosive ref...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.02.074

    authors: Sales TMAL,Nicolau LAD,Rodrigues RLM,Nascimento RR,Paula SM,da Costa-Filho HB,Bezerra FF,Santos GRC,Barros FCN,Alencar POC,Freitas ALP,Nobre-Souza MÂ,Santos AA,Sifrim D,Soares PMG,Silva RO,Souza MHLP

    更新日期:2020-05-01 00:00:00

  • Nano photo scouring and nano photo bleaching of raw cellulosic fabric using nano TiO2.

    abstract::Photo catalytic action of nano TiO(2) for decomposing of some organic compounds is a well known phenomenon. This can be extended to the application on nano TiO(2) on the desized cotton fabric to decompose the hydrophobic impurities and coloring matters of the fabric. This can be nominating as a replacement for the con...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2012.02.018

    authors: Montazer M,Morshedi S

    更新日期:2012-05-01 00:00:00

  • Effect of clay content on morphology and processability of electrospun keratin/poly(lactic acid) nanofiber.

    abstract::This research work has concerned the development of volatile organic compounds (VOCs) removal filters from biomaterials, based on keratin extracted from chicken feather waste and poly(lactic acid) (PLA) (50/50%w/w) blend. Clay (Na-montmorillonite) was also added to the blend solution prior to carrying out an electro-s...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.01.041

    authors: Isarankura Na Ayutthaya S,Tanpichai S,Sangkhun W,Wootthikanokkhan J

    更新日期:2016-04-01 00:00:00

  • Novel composite foam made from starch and water hyacinth with beeswax coating for food packaging applications.

    abstract::A novel composite foam was prepared from native cassava starch and water hyacinth (WH) by baking in a hot mold. The effects of WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis) on the properties of the starch foam were investigated. A starch foam formulation with 5 wt% WH powder exhibited the highest flexural...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.10.007

    authors: Chaireh S,Ngasatool P,Kaewtatip K

    更新日期:2020-12-15 00:00:00