Abstract：
:Self-crosslinkable and injectable hydrogels were fabricated with collagen type I (Col I) and N-hydroxy sulfosuccinimide activated hyaluronic acid (HA-sNHS) at physiological conditions without any initiators or crosslinkers. The physical properties of hydrogels, such as gelation time, swelling property, degradation property and mechanical property could be regulated by adjusting the substitution degree (DS) of HA-sNHS. Chondrocytes were encapsulated into hydrogels and their proliferation, phenotype maintenance and matrix secretion were characterized. The results demonstrated that chondrocytes in hydrogel Col I/HA-sNHS32% in which the DS of HA-sNHS was 32% secreted more cartilage specific matrix than others. The results of animal experiment demonstrated that hydrogels Col I and Col I/HA-sNHS32% both had good biodegradability and cytocompatibility. This study provided a novel and simple method for fabrication of self-crosslinkable and injectable hydrogels with tunable physical properties. It implied that these hydrogels could find some applications in the fields of cell encapsulation and tissue engineering.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Gao Y,Liu Q,Kong W,Wang J,He L,Guo L,Lin H,Fan H,Fan Y,Zhang Xdoi
10.1016/j.ijbiomac.2020.07.319subject
Has Abstractpub_date
2020-12-01 00:00:00pages
2186-2196eissn
0141-8130issn
1879-0003pii
S0141-8130(20)34079-4journal_volume
164pub_type
杂志文章abstract：:Although synthetic antioxidant food additives are widely used in a variety of food products, some of them are suspected of having a noxious effect on human health. As a consequence, much research attention has been focused on developing natural antioxidant compounds from plants. Riang (Parkia timoriana (DC.) Merr.) is...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.09.079
更新日期：2020-12-01 00:00:00
abstract：:In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV)...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.10.121
更新日期：2019-01-01 00:00:00
abstract：:A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper. Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, T...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.05.049
更新日期：2019-08-01 00:00:00
abstract：:In this study, ginger polysaccharide (GP) was obtained from ginger by enzymatic method, its chemical properties and antitumor activity were investigated. The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.10.169
更新日期：2019-02-15 00:00:00
abstract：:Creatine kinase (CK) is a key enzyme involved in intracellular energy homeostasis. The distinct tissue distribution of muscle CK (MMCK) and brain CK (BBCK) implies that they function under conditions facing dissimilar environmental stresses. We found that MMCK and BBCK were significantly different in their stability a...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2010.03.025
更新日期：2010-07-01 00:00:00
abstract：:Cyclina sinensis is an edible clam widely distributed along the coastal waters of Asia. In the present study, a polysaccharide (CSP-1) isolated from C. sinensis was purified by a DEAE-Sepharose Fast Flow column, and it had an average molecular weight of 3.8 × 105 Da and a prevalent component monosaccharide of Glc. The...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.06.020
更新日期：2020-10-15 00:00:00
abstract：:Due to the limited options for topical management of skin cancer, this study aimed at developing and evaluating nanoemulsions (NE) for topical delivery of the cytotoxic agent piplartine (piperlongumine). NEs were modified with chitosan or sodium alginate, and the effects on the physicochemical properties, piplartine d...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.09.167
更新日期：2020-12-15 00:00:00
abstract：:Collagen, a unique connective tissue protein finds extensive application as biocompatible biomaterial in wound healing, as drug carriers, cosmetics, etc. A work has been undertaken to study the stabilization of type I collagen using the plant polyphenol catechin. Catechin treated collagen fibres showed a shrinkage tem...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2005.08.005
更新日期：2005-10-30 00:00:00
abstract：:An antimicrobial packaging material was developed by uniformly embedding 1, 3 and 5% chitosan (w/w) in low density polyethylene matrix using maleic anhydride grafted LDPE as a compatible agent. The materials were mixed by compounding and blown into monolayer films via blown film extrusion. The developed films showed g...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.06.016
更新日期：2015-08-01 00:00:00
abstract：:Bone tissue engineering holds great promise and clinical efficacy for the regeneration of bone defects. In this study, an amphoteric N,O-carboxymethyl chitosan (NOCC) and fucoidan (FD) were covalently cross-linked via an amidation reaction to synthesize NOCC/FD composite hydrogels. The hydrogels were lyophilized and t...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.08.179
更新日期：2018-12-01 00:00:00
abstract：:Bacterial vaginosis (BV) is a common type of vaginal inflammation caused by a proliferation of pathogenic bacteria, among which Mobiluncus curtisii. In our previous studies on M. curtisii genome, we identified the presence of a genomic fragment encoding a 25 kDa pore-forming toxin, the CAMP factor, which is known to b...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.10.107
更新日期：2020-05-01 00:00:00
abstract：:In order to exploit an abundant and cheap carbon source for poly(3-hydroxybutyrate) (PHB) production, the rice husk was pretreated with dilute sulfuric acid and steam flash-explosion to enhance the enzymatic saccharification. The yield of reducing sugars of pretreated rice husk hydrolysate came up to 266.5 mg/g rice h...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.05.218
更新日期：2020-10-01 00:00:00
abstract：:In this study, we developed the new bioactive film from soluble soybean polysaccharide (SSPS) incorporated with different concentrations of cinnamon essential oil nanoemulsions (CNO) and the functional properties of them were evaluated. Then CNO-SSPS film was applied on the meat during refrigerate for 8 days. The use ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.01.145
更新日期：2018-06-01 00:00:00
abstract：:Sulfated polysaccharides are known to display activity against enveloped viruses, such as herpes and dengue. The aim of this work was to assess the antiviral activity of botryosphaeran, a fungal exocellular (1 → 3)(1 → 6)-β-d-glucan devoid of sulfate groups, and its chemically sulfonated derivatives, against herpes si...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.07.084
更新日期：2019-10-01 00:00:00
abstract：:This study aimed to evaluate the in vitro protective effect of topical treatment with a native sulfated polysaccharide of G. caudata (SP-Gc), hydrolyzed (H-SP-Gc), or desulfated (D-SP-Gc) polysaccharide of Gracilaria caudata in esophageal biopsies obtained from GERD patients. Biopsies were obtained from nonerosive ref...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.02.074
更新日期：2020-05-01 00:00:00
abstract：:Naturally occurring polyamines are known to interact with a variety of biomolecules and critically involve in some important physiological processes. They have also been shown to influence protein aggregation in vitro in some cases. The aim of the present study was to investigate how polyamines may influence the struc...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2007.07.018
更新日期：2007-12-01 00:00:00
abstract：:Calorimetry was used to measure the rates of irreversible denaturation of collagen in intact mammalian lens capsules, isothermally stored in water at different temperatures, and the kinetic characteristics of the process determined. At 47.4 degrees C, the number of native collagen molecules declined by a factor of 10 ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(93)90025-h
更新日期：1993-10-01 00:00:00
abstract：:Aliquat-336 (an ionic liquid) impregnated sulphate-crosslinked chitosan (SCC) was prepared for escalating the adsorption of hexavalent chromium through concurrent interaction. The compound obtained was intensively characterized using Fourier transform infra red (FT-IR), X-ray diffraction (XRD), Scanning electron micro...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.07.035
更新日期：2015-09-01 00:00:00
abstract：:In many skeletal support systems of plants and animals, cellulose, chitin, and collagen occur in the form of microfibrils ordered in a chiral nematic fashion (helicoids). However, these structures remain poorly understood due to the many constituents present in biological tissues. Here we report an in vitro system tha...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(05)80008-x
更新日期：1992-06-01 00:00:00
abstract：:A comparison of cross-linked and native gliadin suspensions, with respect to the state of protein globular structure was carried out using small-angle X-ray scattering (SAXS), dynamic light scattering (DLS) and rheological analysis. Gliadin suspensions were also analyzed in the presence and absence of glycerol. DLS an...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.06.041
更新日期：2012-11-01 00:00:00
abstract：:Brachyura is one of the most species rich and highly derived groups among extant crustaceans, with over 7250 known species. However, brachyuran phylogeny remains controversial and requires further study. Here, we combined 103 brachyuran mitogenomes from GenBank with 10 new mitogenomes to describe gene rearrangement pa...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.12.210
更新日期：2021-02-15 00:00:00
abstract：:Myeloid-derived suppressor cells (MDSCs) accumulate in tumor-bearing hosts and play a major role in tumor-induced immunosuppression. The potent modulatory effects of polysaccharides on the innate and adaptive immune system stimulate antitumor responses. In this study, a polysaccharide with an apparent molecular weight...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.04.153
更新日期：2018-08-01 00:00:00
abstract：:Polymer microbeads (MBs) for scrubbing additives have generally been prepared from non-biodegradable synthetic polymers. The worldwide pollution of the marine ecosystem by microplastics urgently demands novel environment-friendly MBs. In this study, Ca-alginate MBs were fabricated by electrospraying an aqueous alginat...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.04.058
更新日期：2019-07-15 00:00:00
abstract：:Among many biodegradable and biocompatible biopolymers, polyhydroxyalkanoates (PHAs), generated by microorganisms, have highly attracted attention in various fields due to their unique physicochemical properties. So far, various types of progresses have been made in environmental and engineering fields by employing PH...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2019.12.181
更新日期：2020-03-01 00:00:00
abstract：:A comprehensive study was conducted to elucidate physicochemical and structural properties of sago starches. Two sago starch granules were oval in shape with an average diameter of 34.41 μm and had C-type polymorph with a crystallinity of about 28.13%. The amylose and resistant starch (RS) contents of two sago starche...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.07.310
更新日期：2020-12-01 00:00:00
abstract：:The structure of an ulvan obtained by water extraction from green seaweed Ulva lactuca was elucidated by using IR, NMR, SEC-MALL and ESIMS methods. The ulvan was also evaluated for its cytotoxic effects on three human cancer cell lines. The results showed that the ulvan was composed of rhamnose, galactose, xylose, man...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.09.040
更新日期：2016-12-01 00:00:00
abstract：OBJECTIVE:Long non-coding RNAs are emerging as key molecules in cancer progression. LncRNA-CCAL has shown to be highly expressed and important in regulating CRC and osteosarcoma development. Nevertheless, the expression and mechanism of CCAL in HCC is still not well understood. METHODS:qRT-PCR and ISH were used to eva...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.12.110
更新日期：2018-04-01 00:00:00
abstract：:Our previous report showed that the novel polysaccharide SAP isolated from the fruiting bodies of Sarcodon aspratus induced Hela cells apoptosis via mitochondrial dysfunction. In this study we found that SAP enhanced immunostimulatory activities of RAW264.7 cells, which was characterized by increased the production of...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.08.147
更新日期：2018-12-01 00:00:00
abstract：:The dawn of molecular biology and recombinant DNA technology arose from our ability to manipulate DNA, including the process of collapse of long extended DNA molecules into nanoparticles of approximately 100 nm diameter. This condensation process is important for the packaging of DNA in the cell and for transporting D...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2017.12.076
更新日期：2018-04-01 00:00:00
abstract：:The principal advantage of the n.m.r. imaging method lies in the specific contrasts which are available. In this work we describe the use of velocity and diffusion contrast methods in biophysical applications and at microscopic spatial resolution. In the first example, involving water-protein interactions, the relatio...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(91)90045-v
更新日期：1991-06-01 00:00:00
