Abstract：
:Cyclina sinensis is an edible clam widely distributed along the coastal waters of Asia. In the present study, a polysaccharide (CSP-1) isolated from C. sinensis was purified by a DEAE-Sepharose Fast Flow column, and it had an average molecular weight of 3.8 × 105 Da and a prevalent component monosaccharide of Glc. The results of methylation analysis and 1D/2D NMR indicated that CSP-1 was a glycogen constructed with α-1,4-Glc and branched at C-6 every 9 Glc residues. In addition, Cong red test suggests CSP-1 was not a helical conformation, and irregular and spherical lumps were observed by AFM. Moreover, CSP-1 was found to possess potent immunostimulatory activity on the basis of its significant abilities to enhance NO production and cytokines (TNF-α, IL-1β and IL-6) secretion in RAW 264.7 macrophages.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Gong Y,Cao C,Ai C,Wen C,Wang L,Zhao J,Han Y,Song S,Xiao Hdoi
10.1016/j.ijbiomac.2020.06.020subject
Has Abstractpub_date
2020-10-15 00:00:00pages
779-786eissn
0141-8130issn
1879-0003pii
S0141-8130(20)33462-0journal_volume
161pub_type
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