Abstract:
:In order to exploit an abundant and cheap carbon source for poly(3-hydroxybutyrate) (PHB) production, the rice husk was pretreated with dilute sulfuric acid and steam flash-explosion to enhance the enzymatic saccharification. The yield of reducing sugars of pretreated rice husk hydrolysate came up to 266.5 mg/g rice husk, much higher than that of untreated rice husk hydrolysate (72.67 mg/g rice husk). This result indicated that the pretreatment can significantly enhance the yield of reducing sugars. Then the hydrolysate was used as a sole carbon source for PHB production by using Cupriavidus necator. Response surface method was used to optimize the fermentation conditions. Under optimum fermentation conditions (carbon source, 31.81 gL-1; nitrogen source, 1.8 gL-1; pH-value, 7.0; temperature, 27.1 °C), the PHB yield was 5.0 gL-1, which was in good agreement with the predicted value.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Zhang Y,Wang L,Li T,Shen Y,Luo Jdoi
10.1016/j.ijbiomac.2020.05.218subject
Has Abstractpub_date
2020-10-01 00:00:00pages
446-455eissn
0141-8130issn
1879-0003pii
S0141-8130(20)33378-Xjournal_volume
160pub_type
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