Acid soaking followed by steam flash-explosion pretreatment to enhance saccharification of rice husk for poly(3-hydroxybutyrate) production.

Abstract:

:In order to exploit an abundant and cheap carbon source for poly(3-hydroxybutyrate) (PHB) production, the rice husk was pretreated with dilute sulfuric acid and steam flash-explosion to enhance the enzymatic saccharification. The yield of reducing sugars of pretreated rice husk hydrolysate came up to 266.5 mg/g rice husk, much higher than that of untreated rice husk hydrolysate (72.67 mg/g rice husk). This result indicated that the pretreatment can significantly enhance the yield of reducing sugars. Then the hydrolysate was used as a sole carbon source for PHB production by using Cupriavidus necator. Response surface method was used to optimize the fermentation conditions. Under optimum fermentation conditions (carbon source, 31.81 gL-1; nitrogen source, 1.8 gL-1; pH-value, 7.0; temperature, 27.1 °C), the PHB yield was 5.0 gL-1, which was in good agreement with the predicted value.

journal_name

Int J Biol Macromol

authors

Zhang Y,Wang L,Li T,Shen Y,Luo J

doi

10.1016/j.ijbiomac.2020.05.218

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

446-455

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(20)33378-X

journal_volume

160

pub_type

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