Abstract：
:Ulvan, a sulfated polysaccharide extracted from the green seaweed genus Ulva, has bioactive properties including an immunomodulating capacity. The immunomodulatory capacity of ulvan from Ulva ohnoi, however, has not been assessed in detail. We depolymerised purified ulvan from U. ohnoi to obtain a range of molecular weight fractions (Mw 7, 9, 13, 21, 209 kDa), which were characterised by constituent sugar analysis, SEC-MALLS, and NMR. Ulvan fractions contained 48.8-54.7 mol% rhamnose, 32.5-35.9 mol% glucuronic acid, 4.5-7.3 mol% iduronic acid, and 3.3-5.6 mol% xylose. 1H and 13C NMR was consistent with hydrolysis occurring at the anomeric centre without further modification to the oligosaccharide structure. The in vitro immunomodulatory effect of ulvan fractions was quantified by measuring levels of inflammatory-mediating signalling molecules released from LPS-stimulated RAW264.7 murine macrophages. All ulvan fractions showed no toxicity on RAW264.7 cells at concentrations below 100 μg mL-1 over 48 h. Secreted interleukin-10 and prostaglandin E2 demonstrated an anti-inflammatory effect by higher molecular weight ulvan fractions at 100 μg mL-1. To a lesser extent, these fractions also enhanced the LPS-induced inflammation through minor increases of IL-1β and IL-6. This study confirms that ulvan from U. ohnoi has a mild in vitro immunomodulatory effect.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Kidgell JT,Glasson CRK,Magnusson M,Vamvounis G,Sims IM,Carnachan SM,Hinkley SFR,Lopata AL,de Nys R,Taki ACdoi
10.1016/j.ijbiomac.2020.02.071subject
Has Abstractpub_date
2020-05-01 00:00:00pages
839-848eissn
0141-8130issn
1879-0003pii
S0141-8130(19)40464-9journal_volume
150pub_type
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