The molecular weight of ulvan affects the in vitro inflammatory response of a murine macrophage.


:Ulvan, a sulfated polysaccharide extracted from the green seaweed genus Ulva, has bioactive properties including an immunomodulating capacity. The immunomodulatory capacity of ulvan from Ulva ohnoi, however, has not been assessed in detail. We depolymerised purified ulvan from U. ohnoi to obtain a range of molecular weight fractions (Mw 7, 9, 13, 21, 209 kDa), which were characterised by constituent sugar analysis, SEC-MALLS, and NMR. Ulvan fractions contained 48.8-54.7 mol% rhamnose, 32.5-35.9 mol% glucuronic acid, 4.5-7.3 mol% iduronic acid, and 3.3-5.6 mol% xylose. 1H and 13C NMR was consistent with hydrolysis occurring at the anomeric centre without further modification to the oligosaccharide structure. The in vitro immunomodulatory effect of ulvan fractions was quantified by measuring levels of inflammatory-mediating signalling molecules released from LPS-stimulated RAW264.7 murine macrophages. All ulvan fractions showed no toxicity on RAW264.7 cells at concentrations below 100 μg mL-1 over 48 h. Secreted interleukin-10 and prostaglandin E2 demonstrated an anti-inflammatory effect by higher molecular weight ulvan fractions at 100 μg mL-1. To a lesser extent, these fractions also enhanced the LPS-induced inflammation through minor increases of IL-1β and IL-6. This study confirms that ulvan from U. ohnoi has a mild in vitro immunomodulatory effect.


Int J Biol Macromol


Kidgell JT,Glasson CRK,Magnusson M,Vamvounis G,Sims IM,Carnachan SM,Hinkley SFR,Lopata AL,de Nys R,Taki AC




Has Abstract


2020-05-01 00:00:00












  • Production of exopolysaccharides by Acinetobacter strains in a controlled fed-batch fermentation process using soap stock oil (SSO) as carbon source.

    abstract::The production of two extracellular capsular heteropolysaccharides by two different Acinetobacter strains has been studied in separate controlled fermentation processes with a view to their industrial applications as specific dispersing agents. The first, emulsan, is an extracellular polyanionic amphipathic heteropoly...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Shabtai Y

    更新日期:1990-04-01 00:00:00

  • Effects of heat-moisture and acid treatments on the structural, physicochemical, and in vitro digestibility properties of lily starch.

    abstract::Starch extracted from lily bulb (Lilium brownii var. Viridulum Baker) was modified via heat-moisture treatment (HMT) at different moisture levels (15-35%) and acid treatment (AT) with hydrochloric acid at five different concentrations (0.25-2.0 M). The effects of HMT and AT on the physicochemical properties and in vit...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Li H,Wang R,Liu J,Zhang Q,Li G,Shan Y,Ding S

    更新日期:2020-04-01 00:00:00

  • Cloning, overexpression, purification of bacteriocin enterocin-B and structural analysis, interaction determination of enterocin-A, B against pathogenic bacteria and human cancer cells.

    abstract::In this present study, a gene (ent-B) encoding the bacteriocin enterocin-B was cloned, overexpressed and purified from Enterococcus faecium por1. The molecular weight of the bacteriocin enterocin-B was observed around 7.2 kDa and exhibited antimicrobial activity against several human pathogenic bacteria. The antimicro...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Ankaiah D,Palanichamy E,Antonyraj CB,Ayyanna R,Perumal V,Ahamed SIB,Arul V

    更新日期:2018-09-01 00:00:00

  • Anti-glioma properties of DVL, a lectin purified from Dioclea violacea.

    abstract::Plant lectins have been studied owing to their structural properties and biological effects that include agglutinating activity, antidepressant-like effect and antitumor property. The results from this work showed the effects of the lectin extracted from the Dioclea violacea plant (DVL) on the C6 rat glioma cell line....

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Nascimento APM,Knaut JL,Rieger DK,Wolin IAV,Heinrich IA,Mann J,Juarez AV,Sosa LDV,De Paul AL,Moreira CG,Silva IB,Nobre CS,Osterne VJS,Nascimento KS,Cavada BS,Leal RB

    更新日期:2018-12-01 00:00:00

  • The role of pH, temperature and nucleation in the formation of cholesteric liquid crystal spherulites from chitin and chitosan.

    abstract::The alpha chitin and chitosan used in the experiments came from crab shell waste (Sigma). This was treated to form a colloidal suspension of chitin or chitosan crystallites. The electrostatic 'charge coat' surrounding the chitin was then manipulated. This was achieved by alteration of the pH of the chitin or chitosan ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Murray SB,Neville AC

    更新日期:1998-04-01 00:00:00

  • Scutellaria baicalensis Georgi polysaccharide ameliorates DSS-induced ulcerative colitis by improving intestinal barrier function and modulating gut microbiota.

    abstract::The aim of this study was to investigate the effect of a polysaccharide from Scutellaria baicalensis Georgi on UC. Gut microbiota dysbiosis is a worldwide problem associating with ulcerative colitis. One homogeneous polysaccharide, named SP2-1, was isolated from Scutellaria baicalensis Georgi. SP2-1 comprised mannose,...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Cui L,Guan X,Ding W,Luo Y,Wang W,Bu W,Song J,Tan X,Sun E,Ning Q,Liu G,Jia X,Feng L

    更新日期:2021-01-01 00:00:00

  • A β-glucan from Grifola frondosa effectively delivers therapeutic oligonucleotide into cells via dectin-1 receptor and attenuates TNFα gene expression.

    abstract::Grifola frondosa is an edible and medicinal mushroom with great nutritional values and bioactivities. In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH. The structure of GFPS was determined wit...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Cui H,Zhu X,Huo Z,Liao B,Huang J,Wang Z,Song C,Hu X,Fang J

    更新日期:2020-04-15 00:00:00

  • A review: Recent advances in ultrasensitive and highly specific recognition aptasensors with various detection strategies.

    abstract::One of the most studied topics in analytical chemistry and physics is to develop bio-sensors. Aptamers are small single-stranded RNA or DNA oligonucleotides (5-25 kDa), which have advantages in comparison to their antibodies such as physicochemical stability and high binding specificity. They are able to integrate wit...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审


    authors: Yan SR,Foroughi MM,Safaei M,Jahani S,Ebrahimpour N,Borhani F,Rezaei Zade Baravati N,Aramesh-Boroujeni Z,Foong LK

    更新日期:2020-07-15 00:00:00

  • The combinatory effect of Cyt1Aa flexibility and specificity against dipteran larvae improves the toxicity of Bacillus thuringensis kurstaki toxins.

    abstract::Cyt1A98 is a novel cytolytic protein, from BUPM98 Bacillus thuringiensis strain, characterized by its synergistic activity with B. thuringiensis kurstaki toxins against lepidopteran larvae. In this study, we evidenced that Cyt1A98 improves the toxicity of B. thuringiensis kurstaki toxins against Aedes aegypti larvae. ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Zribi Zghal R,Frikha F,Elleuch J,Darriet F,Chandre F,Jaoua S,Tounsi S

    更新日期:2019-02-15 00:00:00

  • Novel bio-inspired three-dimensional nanocomposites based on montmorillonite and chitosan.

    abstract::In this study, inspired by nacre-like structural natural shells, novel three-dimensional (3D) nanocomposites based on natural nanoplatelets of montmorillonite (MMT) and polysaccharide of chitosan (CS) were prepared with solution intercalation and self-assembly process. The CS-intercalated-MMT nanoplatelets units acted...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Zheng X,Xu M,Yang S,Omonov S,Huang S,Zhao J,Ruan H,Zeng M

    更新日期:2020-12-15 00:00:00

  • Strategies for screening, purification and characterization of bacteriocins.

    abstract::Bacteriocins are bioactive antimicrobial peptides synthesized in the ribosome of numerous bacteria and released extracellularly. Bacteriocins have the ability to kill or inhibit the growth of prokaryotes and could potentially be useful against pathogens and antibiotic-resistant strains of bacteria. The antimicrobial m...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审


    authors: Zou J,Jiang H,Cheng H,Fang J,Huang G

    更新日期:2018-10-01 00:00:00

  • Ginger polysaccharides induced cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.

    abstract::In this study, ginger polysaccharide (GP) was obtained from ginger by enzymatic method, its chemical properties and antitumor activity were investigated. The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Wang Y,Wang S,Song R,Cai J,Xu J,Tang X,Li N

    更新日期:2019-02-15 00:00:00

  • Quantitative proteomics to reveal the composition of Southern India spectacled cobra (Naja naja) venom and its immunological cross-reactivity towards commercial antivenom.

    abstract::Indian cobra (Naja naja) envenomation is frequently reported across Indian subcontinent. Geographical differences in the venom composition of a particular species of snake often leads to inconsistencies in the antivenom neutralization. Consequently, determining the venom proteome from every locale is necessary for the...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Chanda A,Mukherjee AK

    更新日期:2020-10-01 00:00:00

  • Synthesis and characterization of diethoxy phosphoryl chitosan.

    abstract::A novel diethoxy phosphoryl chitosan was successfully synthesized via Atherton-Todd reaction of chitosan with diethyl phosphite, and the characterization was carried out by FT IR, NMR, ICP, XRD, TG and SEM, respectively. The FT IR, (13)C NMR and (31)P NMR results showed that diethoxy phosphoryl group was grafted onto ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Ma L,Li G,Li L,Liu P

    更新日期:2010-11-01 00:00:00

  • Chitosan-based nanoparticles for tumor-targeted drug delivery.

    abstract::Nanoparticles (NPs) have immense potential as drug-delivery carriers for cancer therapy due to their unique physicochemical properties. These particles can improve the pharmacological and therapeutic properties of the anti-cancer drugs by controlling release rates and targeted delivery process, which eliminate the lim...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审


    authors: Prabaharan M

    更新日期:2015-01-01 00:00:00

  • Rheological, textural and emulsifying properties of an exopolysaccharide produced by Mesorhizobium loti grown on a crude glycerol-based medium.

    abstract::In the present study, a new extracellular polysaccharide (EPS-M816) was obtained during the growth of Mesorhizobium loti Semia 816 on a crude glycerol-based medium. EPS-M816 precipitate mainly consisted of carbohydrates (82.54%) and proteins (11.31%), and the weight average molecular weight was estimated at 1.646 × 10...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: de Oliveira JM,Amaral SA,Burkert CAV

    更新日期:2018-12-01 00:00:00

  • Preparation and characterization of D. opposita Thunb polysaccharide-zinc inclusion complex and evaluation of anti-diabetic activities.

    abstract::In the present work, Dioscorea opposita Thunb polysaccharide-zinc (DPS-zinc) inclusion complex was synthesized for the first time and its anti-diabetes activities against Streptozotocin (STZ)-induced diabetic rats were assayed. The detail structural and morphological analysis of as-prepared DPS-zinc complex was done b...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Zhang Y,Khan MZH,Yuan T,Zhang Y,Liu X,Du Z,Zhao Y

    更新日期:2019-01-01 00:00:00

  • Presence of N-L-lactyl-D-perosamine residue in the sheath-forming polysaccharide of Thiothrix fructosivorans.

    abstract::Thiothrix fructosivorans forms a microtube (sheath) that encloses a line of cells. This sheath is an assemblage of [→4)-GlcN-(1→4)-Glc-(1→]n with side chains of Rha4N-(1→3)-Fuc(1→ at position 3 of Glc. The sheath-forming polysaccharide (SFP) may have some substitutions but this is not yet confirmed. To investigate the...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Kawasaki Y,Kondo K,Narizuka R,Endo T,Katahira M,Kawamura I,Sato M,Takeda M

    更新日期:2016-01-01 00:00:00

  • Detection, synthesis, structure, and function of oligo(3-hydroxyalkanoates): contributions by synthetic organic chemists.

    abstract::Two types of the biological macromolecules poly(R-3-hydroxyalkanoates) have been identified: the high-molecular-weight microbial storage material (sPHA) and a short-chain variety, consisting of butyrate and valerate residues, complexed with other biomacromolecules such as calcium polyphosphate or proteins (cPHB/PHV). ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审


    authors: Seebach D,Fritz MG

    更新日期:1999-06-01 00:00:00

  • Cyclodextrin based ternary system of modafinil: Effect of trimethyl chitosan and polyvinylpyrrolidone as complexing agents.

    abstract::Modafinil is an approved drug for the treatment of narcolepsy and have a strong market presence in many countries. The drug is widely consumed for off-label uses and currently listed as a restricted drug. Modafinil has very low water solubility. To enhance the aqueous solubility of modafinil by the formation of a tern...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Patel P,Agrawal YK,Sarvaiya J

    更新日期:2016-03-01 00:00:00

  • First evidence of formation of pre-molten globule state in myoglobin: A macromolecular crowding approach towards protein folding in vivo.

    abstract::Myoglobin is known to show formation of intermediate states under various environmental conditions, in spite of that, this is the first evidence of formation pre-molten globule (PMG) in myoglobin. Polyethylene glycol (PEG) of various molecular sizes shows assorted effects on different proteins. Out of too short and to...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Parray ZA,Ahamad S,Ahmad F,Hassan MI,Islam A

    更新日期:2019-04-01 00:00:00

  • Characterization of sodium caseinate/Hydroxypropyl methylcellulose concentrated emulsions: Effect of mixing ratio, concentration and wax addition.

    abstract::The effects of mixing ratio (1:1, 2:1 and 4:1 sodium caseinate: Hydroxypropyl methylcellulose, CN:HPMC), HPMC concentration (0, 0.6 and 1.2 wt%), CN concentration (0, 1.25 and 2.5) and beeswax addition (3%) on the physical stability of concentrated O/W emulsions (φoil = 0.6) were investigated. The emulsion stability, ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Alizadeh L,Abdolmaleki K,Nayebzadeh K,Bahmaei M

    更新日期:2019-05-01 00:00:00

  • Crystallinity, thermal and pasting properties of starches from different potato cultivars grown in Brazil.

    abstract::Starches from different potato cultivars were characterized as amylose and phosphorus content, crystallinity, thermal and paste properties. Statistical analysis of amylose content showed difference between starches samples and the cultivars Asterix and BRS Clara showed higher contents than others. Phosphorus content r...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Dos Santos TP,Leonel M,Garcia ÉL,do Carmo EL,Franco CM

    更新日期:2016-01-01 00:00:00

  • Fabrication of biodegradable textile scaffold based on hydrophobized hyaluronic acid.

    abstract::In this work, we report on the preparation of a novel biodegradable textile scaffold made of palmitoyl-hyaluronan (palHA). Monofilament fibres of palHA with a diameter of 120μm were prepared by wet spinning. The wet-spun fibres were subsequently processed into a warp-knitted textile. To find a compromise between swell...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Zapotocky V,Pospisilova M,Janouchova K,Svadlak D,Batova J,Sogorkova J,Cepa M,Betak J,Stepankova V,Sulakova R,Kulhanek J,Pitucha T,Vranova J,Duffy G,Velebny V

    更新日期:2017-02-01 00:00:00

  • Roles of galectin-3 in metabolic disorders and tumor cell metabolism.

    abstract::The galactoside-binding protein galectin-3 is commonly overexpressed by cancer cells and promotes cancer progression and metastasis. Over the past few years, evidence has emerged that galectin-3 is also overexpressed in several metabolic malfunction conditions such as diabetes, obesity and atherosclerosis and is invol...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审


    authors: Li YS,Li XT,Yu LG,Wang L,Shi ZY,Guo XL

    更新日期:2020-01-01 00:00:00

  • Optimization of brain targeted chitosan nanoparticles of Rivastigmine for improved efficacy and safety.

    abstract::The study aims at formulation and optimization brain targeted nanoparticles (NP) of Rivastigmine (RT) to improve its therapeutic potential and to verify its safety profile. The NP were optimized using a two factor three level (3(2)) central composite design aiming to minimize particle size; maximize zeta potential and...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Nagpal K,Singh SK,Mishra DN

    更新日期:2013-08-01 00:00:00

  • The oxidation of potato starch by Electro-Fenton system in the presence of Fe(II) ions.

    abstract::An efficient method was developed to prepare oxidized potato starch under the pasting point by Electro-Fenton system, in which a complex of sodium citrate and Fe2+ (SC-Fe2+) was used as catalyst, and H2O2 was used as oxidant. In the preparation process the oxidation degree of starch was controlled by the molar ratios ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Dang X,Chen H,Shan Z,Zhen W,Yang M

    更新日期:2019-01-01 00:00:00

  • Thermal stress resistance and aging effects of Panax notoginseng polysaccharides on Caenorhabditis elegans.

    abstract::Panax notoginseng attract public attention due to their potential biomedical properties and corresponding health benefits. The present study investigated the anti-aging and thermal stress resistance effects of polysaccharides from P. notoginseng on Caenorhabditis elegans. Results showed polysaccharides had little scav...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Feng S,Cheng H,Xu Z,Shen S,Yuan M,Liu J,Ding C

    更新日期:2015-11-01 00:00:00

  • Molecular structure of carrageenans and kappa oligomers: a Raman spectroscopic study.

    abstract::The Raman spectra of kappa and iota carrageenan, a desulphated furcellaran and a series of oligomers have been compared in the region 700-1500 cm-1. Spectral differences depending on the amount and the location of the sulphate group on the ring, the chain length, the nature of the counterion and the conformation are d...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Malfait T,Van Dael H,Van Cauwelaert F

    更新日期:1989-10-01 00:00:00

  • Two classes of cytochrome P450 reductase genes and their divergent functions in Camptotheca acuminata Decne.

    abstract::Cytochrome P450 monooxygenases (CYP450s) and their auxiliary cytochrome P450 reductases (CPRs) are important and commonly involved in the biosynthesis of camptothecin (CPT). To better understand the possible functions of CPRs in planta, we first isolated two CaCPRs genes from Camptotheca acuminata. Sequence analysis r...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Jin Z,Cong Y,Zhu S,Xing R,Zhang D,Yao X,Wan R,Wang Y,Yu F

    更新日期:2019-10-01 00:00:00