Abstract:
:Three acidic polysaccharides (PPQA2, PPQA4 and PPQA5) were successfully purified from the water-extracted crude polysaccharides of Panax quinquefolius by combination of DEAE Sepharose and Sephacryl S-300 chromatography. The average molecular weights (Mws) of PPQA2, PPQA4 and PPQA5 were 2.3×10(4) Da, 1.2×10(5) Da, and 5.3×10(3) Da, respectively. Monosaccharides components analysis indicated that PPQA2 and PPQA5 were composed of arabinose, rhammose, mannose, galactose, glucose, galacturonic acid and glucuronic acid in a molar percent of 8.0:4.0:2.9:7.2:12.5:26.6:38.8 and 8.5:3.2:5.3:10.8:32.4:15.5:24.4. PPQA4 was composed of arabinose, rhammose, mannose, galactose, glucose and glucuronic acid in a molar percent of 19.7:5.1:8.1:23.9:41.3:2.0. The (13)C NMR spectra indicated the existence of O-acetyl groups in PPQA2 and PPQA5, while absence in PPQA4. The in vitro study showed that PPQA2, PPQA4 and PPQA5 were all able to stimulate the production of secretory molecules (NO, TNF-α, and IL-6) of RAW264.7 murine macrophages in a dose-dependent manner. These findings suggest that the three acidic polysaccharides isolated in our study have immunopotentiating effects on macrophages and should be used as a beneficial health food.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Wang L,Yao Y,Sang W,Yang X,Ren Gdoi
10.1016/j.ijbiomac.2015.06.007subject
Has Abstractpub_date
2015-09-01 00:00:00pages
77-86eissn
0141-8130issn
1879-0003pii
S0141-8130(15)00409-2journal_volume
80pub_type
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