当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > C-terminal modification of monoclonal antibody drugs: amidated species as a general product-related substance.

C-terminal modification of monoclonal antibody drugs: amidated species as a general product-related substance.

Abstract：

:Twelve therapeutic mAbs, comprising 10 IgG1s and 2 IgG4s, were analyzed by a peptide mapping technique to detect and quantify C-terminal modifications. C-terminal amidated structures were found in 8 out of the 12 mAbs. An in vitro study using a commercially available peptidylglycine alpha-amidating monooxygenase (PAM) revealed that both IgG1 and IgG4 can be substrates for PAM. This study showed that C-terminal amidation is a general C-terminal modification on the heavy chains of therapeutic mAbs and that C-terminal amidation of mAbs can be catalyzed by a certain PAM(s) in the Chinese hamster ovary (CHO) cells that are widely used for manufacturing therapeutic mAbs.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Tsubaki M,Terashima I,Kamata K,Koga A

doi

10.1016/j.ijbiomac.2012.09.016

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

139-47

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(12)00370-4

journal_volume

52

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Effect of chitin/silk fibroin nanofibrous bicomponent structures on interaction with human epidermal keratinocytes.

    abstract：:To fabricate a biomimetic nanostructured bicomponent scaffolds, two types of chitin/silk fibroin (SF) nanofibrous scaffolds (blend scaffolds and hybrid scaffolds) were prepared by electrospinning or simultaneous electrospinning of chitin/SF solutions. The chitin/SF bicomponent scaffolds were after-treated with water v...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2007.12.004

    authors： Yoo CR,Yeo IS,Park KE,Park JH,Lee SJ,Park WH,Min BM

    更新日期：2008-05-01 00:00:00

  • Super and selective adsorption of cationic dyes using carboxylate-modified lignosulfonate by environmentally friendly solvent-free esterification.

    abstract：:Herein, following the strategy of sustainable, environment protection, circular economy development, carboxylate-modification lignosulfonate polymer (M-LSP) was synthesized from lignosulfonate by solvent-free esterifying with maleic anhydride (MA) by one step, and was used to remove the dyes by adsorption. FT-IR and X...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.05.037

    authors： Tang Y,Lin T,Ai S,Li Y,Zhou R,Peng Y

    更新日期：2020-09-15 00:00:00

  • Surface chemical functionalization of starch nanocrystals modified by 3-aminopropyl triethoxysilane.

    abstract：:A comparative experiment about the 3‑aminopropyl triethoxysilane (APTES) modification of waxy potato starch nanocrystals (WPSNCs) and waxy maize starch nanocrystals (WMSNCs) was employed in the present study. The purpose of surface chemical functionalization was to introduce functional groups and improve hydrophobicit...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.12.200

    authors： Hao Y,Chen Y,Xia H,Gao Q

    更新日期：2019-04-01 00:00:00

  • Characterization of pH-induced transitions of Entamoeba histolytica D-phosphoglycerate dehydrogenase.

    abstract：:Entamoeba histolytica D-phosphoglycerate dehydrogenase (EhPGDH) exists as a functionally active homodimer at pH 7. Our earlier studies have shown that ionic interactions are essentially required for the oligomeric status and activity of the protein. Present study focuses on pH associated structural modulations of EhPG...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.04.058

    authors： Mishra V,Kumar A,Ali V,Zhang KY,Nozaki T

    更新日期：2015-08-01 00:00:00

  • The effect of carotenoids obtained from saffron on histone H1 structure and H1-DNA interaction.

    abstract：:It is already known that transcriptional activation of genes occurs due to the H1 dissociation from linker DNA; hence, histone H1-DNA complex is considered as a model of chromatin. Anticancer property of saffron and its carotenoids has already been reported. The present study aimed at investigating the effect of saffr...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2005.05.008

    authors： Ashrafi M,Bathaie SZ,Taghikhani M,Moosavi-Movahedi AA

    更新日期：2005-09-15 00:00:00

  • Antioxidant and antimicrobial carboxymethyl cellulose films containing Zataria multiflora essential oil.

    abstract：:The present study describes the physical, antioxidant, and antimicrobial properties of biodegradable films prepared by incorporating different concentrations (1, 2, and 3% v/v) of Zataria multiflora Boiss (avishan-e shirazi) essential oil (ZEO) into carboxymethyl cellulose (CMC) film. The films' tensile strength, elon...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.09.006

    authors： Dashipour A,Razavilar V,Hosseini H,Shojaee-Aliabadi S,German JB,Ghanati K,Khakpour M,Khaksar R

    更新日期：2015-01-01 00:00:00

  • Preparation and characterization of bionanocomposite films based on potato starch/halloysite nanoclay.

    abstract：:In this research casting method was used to prepare potato starch based bio-nanocomposite films with halloysite nanoclay as the reinforcing materials. The composition included potato starch with 40% (w/w) of a mixture of sorbitol/glycerol (weight ratio of 3 to 1as plasticizer) with nanoclay (0-5% w/w). The films were ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.04.009

    authors： Sadegh-Hassani F,Mohammadi Nafchi A

    更新日期：2014-06-01 00:00:00

  • Characterization and comparative studies of galactomannans from Bauhinia vahlii, Delonix elata, and Peltophorum pterocarpum.

    abstract：:The present investigation determines the extraction and characterization of seed galactomannans from the Leguminosae taxa of Bauhinia vahlii, Delonix elata, and Peltophorum pterocarpum. The seed galactomannans presented a total yield of 30.82, 24.01 and 25.25% with a Man/Gal ratios of 4.21:1, 2.55:1 and 3.03:1 for B. ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.05.080

    authors： Jamir K,Badithi N,Venumadhav K,Seshagirirao K

    更新日期：2019-08-01 00:00:00

  • A starch-based stimuli-responsive magnetite nanohydrogel as de novo drug delivery system.

    abstract：:A novel starch-based stimuli-responsive magnetite nanohydrogel (MNHG), namely Fe3O4-g-[poly(N-isopropylacrylamide-co-maleic anhydride)]@strach; Fe3O4-g-(PNIPAAm-co-PMA)@starch, was successfully developed for targeted delivery of doxorubicin (DOX) as an anticancer drug. First, magnetite nanoparticles (MNPs) was modifie...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.05.211

    authors： Massoumi B,Mozaffari Z,Jaymand M

    更新日期：2018-10-01 00:00:00

  • Anti-tumor and anti-virus activity of polysaccharides extracted from Sipunculus nudus(SNP) on Hepg2.2.15.

    abstract：:Many polysaccharides have biological activities and have been investigated for their antitumor effects. In this study, we investigated the anti-tumor activity and anti-virus activity of SNP-the water-soluble polysaccharides extracted from Sipunculus nudus on Hepg2.2.15. Flow cytometry analysis demonstrated that SNP in...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.03.022

    authors： Su J,Jiang L,Wu J,Liu Z,Wu Y

    更新日期：2016-06-01 00:00:00

  • Antimicrobial cerium ion-chitosan crosslinked alginate biopolymer films: A novel and potential wound dressing.

    abstract：:Wound dressings require good antiseptic properties, mechanical strength and, more trustably, natural material ingredients. Antimicrobial properties of cerium ions and chitosan are known and alginate based wound dressings are commercially available. In this study, the advantages of these materials were combined and alg...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.07.144

    authors： Kaygusuz H,Torlak E,Akın-Evingür G,Özen İ,von Klitzing R,Erim FB

    更新日期：2017-12-01 00:00:00

  • A novel quaternized chitosan-melamine-glutaraldehyde resin for the removal of nitrate and phosphate anions.

    abstract：:A terpolymer of chitosan-melamine-glutaraldehyde was prepared for the first time and it was quaternized with glycidyl trimethyl ammonium chloride (GTMAC). The quaternized chitosan-melamine-glutaraldehyde resin (QCMGR) was found to be effective for the removal of nitrate and phosphate oxyanions. The resin was character...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.11.036

    authors： Sowmya A,Meenakshi S

    更新日期：2014-03-01 00:00:00

  • Anticancer effect of atorvastatin nanostructured polymeric micelles based on stearyl-grafted chitosan.

    abstract：:The purpose of this study was to develop a new therapeutic approach for atorvastatin (ATV) adopting nanostructured polymeric micelles for its controlled delivery to the cancer cells. Amphiphilic block copolymers of stearyl chitosan (SC) and sulfated stearyl chitosan (S-SC) that could self assemble to form polymeric mi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2012.05.026

    authors： Mekhail GM,Kamel AO,Awad GA,Mortada ND

    更新日期：2012-11-01 00:00:00

  • Enhancement in in vitro anti-angiogenesis activity and cytotoxicity in lung cancer cell by pectin-PVP based curcumin particulates.

    abstract：:The aim of this work was to prepare pectin-poly (vinyl pyrrolidone) [PVP] based curcumin particulates to enhance the anticancer potential of curcumin, solubility and allow its localized controlled release. Pectin-PVP based curcumin particulates (PECTIN-PVP CUR) were prepared by spray drying technique in different rati...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.05.170

    authors： Gaikwad D,Shewale R,Patil V,Mali D,Gaikwad U,Jadhav N

    更新日期：2017-11-01 00:00:00

  • Isolation and characterization of Populus xyloglucan endotransglycosylase/hydrolase (XTH) involved in osmotic stress responses.

    abstract：:Xyloglucan endotransglycosylase/hydrolase (XTH) belongs to the GH16 subfamily of the glycoside hydrolases of carbohydrate active enzymes and plays an important role in the structure and function of plant cell walls. In this study, 11 members of the XTH gene family were cloned from Populus tomentosa. A bioinformatics a...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.11.099

    authors： Jiang Y,Li Y,Lu C,Tang Y,Jiang X,Gai Y

    更新日期：2020-07-15 00:00:00

  • Preparation and characterization of polymeric nanoparticles surface modified with chitosan for target treatment of colorectal cancer.

    abstract：:5-Fluorouracil (5-FU) loaded chitosan (C) coated polylactic-co-glycolic acid (PLGA) nanoparticles [NPs] (C-5-FU PLGA NPs) and polycaprolactone [PCL] (C-5-FU PCL NPs) were employed as the carriers for cancer treatment. The prepared NPs showed the spherical shape of NPs with the particle size in the range of 188.1-302.2...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.11.098

    authors： Badran MM,Mady MM,Ghannam MM,Shakeel F

    更新日期：2017-02-01 00:00:00

  • A polysaccharide isolated from Sphallerocarpus gracilis protects PC12 cells against hydrogen peroxide-induced injury.

    abstract：:Sphallerocarpus gracilis is a medicinal and edible plant in China with high nutritional value. We examined the structure and bioactivity of a new polysaccharide fraction purified from S. gracilis named SGP-1. This polysaccharide possessed an average molecular weight of 4.916 × 105 and was primarily composed of arabino...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.11.208

    authors： Guo J,Liu Q,Wang C,Shi J,Zhang J

    更新日期：2019-05-15 00:00:00

  • Epimedium polysaccharide and propolis flavone can synergistically stimulate lymphocyte proliferation in vitro and enhance the immune responses to ND vaccine in chickens.

    abstract：:Four prescriptions, epimedium flavone plus propolis flavone (EF-PF), epimedium flavone plus propolis extracts (EF-PE), epimedium polysaccharide plus propolis flavone (EP-PF) and epimedium polysaccharide plus propolis extracts (EP-PE), were prepared and their immune-enhancing effects were compared. In test in vitro, th...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2010.05.017

    authors： Fan Y,Hu Y,Wang D,Guo Z,Zhao X,Guo L,Zhao B,Zhang J,Wang Y,Nguyen TL

    更新日期：2010-08-01 00:00:00

  • Oral administration of chitin and chitosan prevents peanut-induced anaphylaxis in a murine food allergy model.

    abstract：:Peanut allergy is IgE-mediated type-I hypersensitivity, and T helper 2 cytokines are central to those pathogenesis. We investigated the effects of the administration of chitin and chitosan on peanut-induced hypersensitivities in mouse food allergy models. Chitin and chitosan protected mice against peanut-induced anaph...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.06.017

    authors： Bae MJ,Shin HS,Kim EK,Kim J,Shon DH

    更新日期：2013-10-01 00:00:00

  • Structural plasticity associated with the beta-propeller architecture.

    abstract：:The beta-propeller architecture observed in protein tertiary structure and classified into the five different types according to number of 'blades' (or beta-sheets) and a sixth type classified according to the secondary structure composition of the blades (the beta beta alpha beta-molecular unit) is characterized by v...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2004.03.003

    authors： Guruprasad K,Dhamayanthi P

    更新日期：2004-04-01 00:00:00

  • Starch based biodegradable graft copolymer for the preparation of silver nanoparticles.

    abstract：:The synthesis and characterization of a novel biodegradable graft copolymer based on partially hydrolyzed polymethylacrylate (PMA) grafted amylopectin (AP) was reported which was developed for the synthesis of silver nanoparticles from silver nitrate solution by facile green technique. The prepared graft copolymer was...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.07.046

    authors： Das S,Sasmal D,Pal S,Kolya H,Pandey A,Tripathy T

    更新日期：2015-11-01 00:00:00

  • Structure of the polysaccharide isolated from the sheath of Sphaerotilus natans.

    abstract：:A polysaccharide was isolated from the sheath of a sheathed bacterium, Sphaerotilus natans. The sheath polysaccharide (SPS) was composed of D-glucose and D-(N-acetyl)galactosamine in molar ratios of 1:4. Methylation linkage analysis revealed the presence of the residues of 4-linked glucose, 4-linked (N-acetyl)galactos...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2003.08.008

    authors： Takeda M,Nakamori T,Hatta M,Yamada H,Koizumi J

    更新日期：2003-12-01 00:00:00

  • On the temperature-induced coil to globule transition of poly-N-isopropylacrylamide in dilute aqueous solutions.

    abstract：:Poly-N-isopropylacrylamide (PNIPAM) is a chemical isomer of poly-leucine, having the polar peptide group in the side-chain rather than in the backbone. It has been demonstrated experimentally that PNIPAM dissolved in aqueous solution undergoes a collapse transition from coil to globule on increasing temperature above ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/s0141-8130(99)00122-1

    authors： Graziano G

    更新日期：2000-03-16 00:00:00

  • Effect of roasting process on enzymes inactivation and starch properties of highland barley.

    abstract：:Roasting may be beneficial to extend the shelf life by inactivating the lipases and peroxidases in highland barley, but it may lead to the deterioration of starch quality. In this study, the pre-treatment techniques of pearling and tempering were tried to improve the efficiency of inactivation, and different roasting ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.09.180

    authors： Zhao B,Shang J,Liu L,Tong L,Zhou X,Wang S,Zhang Y,Wang L,Zhou S

    更新日期：2020-12-15 00:00:00

  • Sugar compositions, α-glucosidase inhibitory and amylase inhibitory activities of polysaccharides from leaves and flowers of Camellia sinensis obtained by different extraction methods.

    abstract：:The sugar compositions, α-glucosidase inhibitory and α-amylase inhibitory activities of polysaccharides from leaves and flowers of green tea (Camellia sinensis) obtained by hot water extraction (HWE), boiled water extraction (BWE) and enzymatic extraction (EE) were investigated. The yields, sugar contents and monosacc...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2010.07.007

    authors： Wang Y,Yang Z,Wei X

    更新日期：2010-11-01 00:00:00

  • Tuned cell attachments by double-network hydrogels consisting of glycol chitosan, carboxylmethyl cellulose and agar bearing robust and self-healing properties.

    abstract：:Herein we present a tuned cell attachment on self-healable double network hydrogel bearing dynamic covalent bonds and hydrogen bonds. Agar formed first network, while glycol chitosan and oxidized carboxylmethyl cellulose formed second network in the resultant double network hydrogel. Because of the simple one-pot prep...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.05.053

    authors： Cho IS,Ooya T

    更新日期：2019-08-01 00:00:00

  • Perspectives on progressive strategies and recent trends in the production of recombinant human factor VIII.

    abstract：:Factor VIII (fVIII), a glycoprotein cofactor, plays a crucial role in the intrinsic blood coagulation pathway. As one of the most essential blood clotting factors known today, fVIII is the largest and most complex commercialized therapeutic protein used in the treatment of hemophilia A, an X-linked recessive disorder....

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2018.07.164

    authors： Mannully ST,L N R,Pulicherla KK

    更新日期：2018-11-01 00:00:00

  • Composition and bioactivity of polysaccharides from Inula britannica flower.

    abstract：:In this paper, the composition and biological activities of polysaccharides from Inula britannica flower IBP obtained by water extraction were investigated. The properties and chemical compositions of IBP were analyzed with HPLC and IR methods. The results showed that IBP consisted of two kinds of polysaccharides with...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2012.06.021

    authors： Hong T,Zhao J,Dong M,Meng Y,Mu J,Yang Z

    更新日期：2012-11-01 00:00:00

  • Anti-inflammatory activity of polysaccharides from Phellinus linteus by regulating the NF-κB translocation in LPS-stimulated RAW264.7 macrophages.

    abstract：:Inflammatory response remains one of the most common and serious complications of disease. In order to profound understanding relationship between Phellinus linteus polysaccharides (TCM) and inflammation, inflammatory cell model was constructed by LPS acting RAW264.7 cell line. The results showed that TCM could decrea...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.02.023

    authors： Xie Z,Wang Y,Huang J,Qian N,Shen G,Chen L

    更新日期：2019-05-15 00:00:00

  • Non-classical secretion of 1,4-alpha-glucan branching enzymes without signal peptides in Escherichia coli.

    abstract：:1,4-α-Glucan branching enzyme (GBE, EC. 2.4.1.18), which plays a key role in the synthesis of starch and glycogen, has been overexpressed in E. coli as an intracellular enzyme by many researchers. In this study, it was found that the GBEs from Geobacillus thermoglucosidans and Rhodothermus obamensis were secreted into...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.04.002

    authors： Xin C,Ban X,Gu Z,Li C,Cheng L,Hong Y,Li Z

    更新日期：2019-07-01 00:00:00