Abstract:
:In the present study, efficient enzymatic methods were developed using a recombinant metagenomic lipase (LipR1) for the synthesis of corresponding esters by the transesterification of five different pharmaceutically important secondary alcohols. The recombinant lipase (specific activity=87m6U/mg) showed maximum conversion in presence of ionic liquid with Naphthyl-ethanol (eeP=99%), Indanol and Methyl-4 pyridine methanol (eeS of 98% and 99%) respectively in 1h. Vinyl acetate was found as suitable acyl donor in transesterification reactions. It was interesting to observe that maximum eeP of 85% was observed in just 15min with 1-indanol. As this enzyme demonstrated pharmaceutical applications, attempts were made to scale up the enzyme production on a pilot scale in a 5litre bioreactor. Different physical parameters affecting enzyme production and biomass concentration such as agitation rate, aeration rate and inoculum concentration were evaluated. Maximum lipase activity of 8463U/ml was obtained at 7h of cultivation at 1 lpm, 300rpm and 1.5% inoculum.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Kumar R,Banoth L,Banerjee UC,Kaur Jdoi
10.1016/j.ijbiomac.2016.10.088subject
Has Abstractpub_date
2017-02-01 00:00:00pages
995-1003eissn
0141-8130issn
1879-0003pii
S0141-8130(16)30836-4journal_volume
95pub_type
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