Abstract:
:A novel homogeneous polysaccharide fraction (APS) was extracted from Astragalus by hot water and purified by Sephadex G-100 and G-75 column. Its molecular weight was 693kDa. APS and APS with ultra-high pressure treatment exhibited significant inhibitory abilities on a-glucosidase, inhibition rate from high to low in order was 400MPa-APS, 300MPa-APS, 500MPa-APS and APS. The inhibition percentage of 400MPa-APS (1.5mg/mL) was 49% (max.). This suggested that the inhibitory activity of APS on a-glucosidase was improved by ultra-high pressure treatment. FT-IR, SEM, CD spectra, atomic force microscope and Congo red test analysis of APS and 400MPa-APS showed ultra-high pressure treatment didn't change the preliminary structure but had an effect on its advanced structure.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Zhu ZY,Luo Y,Dong GL,Ren YY,Chen LJ,Guo MZ,Wang XT,Yang XY,Zhang Ydoi
10.1016/j.ijbiomac.2016.03.024subject
Has Abstractpub_date
2016-06-01 00:00:00pages
570-6eissn
0141-8130issn
1879-0003pii
S0141-8130(16)30248-3journal_volume
87pub_type
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