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Bioconversion of crude glycerol to polyhydroxyalkanoate by Bacillus thuringiensis under non-limiting nitrogen conditions.

Abstract：

:Glycerol has emerged as a cheap waste material due to blooming biodiesel manufacturing units worldwide. The need is to exploit the crude glycerol (CG) to produce useful products such as polyhydroxyalkanoate (PHA). Bacillus thuringiensis EGU45 was found to produce 1.5-3.5 gP HA L(-1) from feed containing 1-10% CG (vv(-1)) and nutrient broth (NB, 125 mL) without any acclimatization. B. thuringiensis EGU45 could produce PHA at the rate of 1.54-1.83 g L(-1), from 1% CG (vv(-1)) on media having high nitrogen contents: (i) NB, (ii) NB+0.5% NH4Cl (wv(-1)), and (iii) peptone+yeast extract+0.5% NH4Cl (wv(-1)). B. thuringiensis EGU45 was able to produce co-polymer of P(3HB-co-3HV) with 13.4% 3HV content on high N containing feed supplemented with propionic acid. This is the first report demonstrating the abilities of B. thuringiensis to convert CG into PHA co-polymer under non-limiting N conditions.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Kumar P,Ray S,Patel SK,Lee JK,Kalia VC

doi

10.1016/j.ijbiomac.2015.03.046

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

9-16

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(15)00198-1

journal_volume

78

pub_type

杂志文章

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