Abstract:
:In this work, glucose oxidase (GOD) and catalase (CAT) were co-immobilized on novel silica inverse opals (IO-SiO2) through sol-gel process. The immobilized bi-enzyme system named GOD/CAT@IO-SiO2 was successfully fabricated and characterized. Morphology characterization indicated that GOD/CAT@IO-SiO2 had hierarchical porous structure, and the pore diameter of macroporous and mesoporous were 500±50nm and 6.8nm, respectively. The macrospores were connected through windows of 100±30nm. The results of stability tests indicated that both acid (or base) resistance and thermal tolerance of GOD/CAT@IO-SiO2 were improved. When GOD/CAT@IO-SiO2 was used to remove glucose from commercial isomaltooligosaccharide (IMO), the immobilized bi-enzyme system exhibited the good performance. The removal efficiency of glucose reached up to 98.97% under the conditions of GOD/CAT activity ratio of 1:30, the amount of enzyme of 68.8mg, reaction time of 9.39h, reaction temperature of 35.2°C and pH of 7.05. After reused 6 times, 79.19% of removal efficiency could be still retained. The present work demonstrates that the immobilized bi-enzyme (GOD/CAT@IO-SiO2) is not only a very promising system for glucose removal but also has great potential for applications in production of gluconic acid, preparation of biosensors, enzyme bioreactors, etc.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Zhao B,Zhou L,Ma L,He Y,Gao J,Li D,Jiang Ydoi
10.1016/j.ijbiomac.2017.10.074subject
Has Abstractpub_date
2018-02-01 00:00:00pages
2034-2043issue
Pt Beissn
0141-8130issn
1879-0003pii
S0141-8130(17)33132-Xjournal_volume
107pub_type
杂志文章abstract::Green biocomposites based on corn starch plasticized with isosorbide and glycerol and filled with microcrystalline cellulose (MCC) were processed. The structural interactions, dynamomechanical properties, water absorption, and soil biodegradability were investigated by different techniques considering the effect of th...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.075
更新日期:2020-12-01 00:00:00
abstract::Hericium erinaceus polysaccharide (HEP) is a bioactive substance present in the fruiting bodies of H. erinaceus. Previously we have shown that HEP can repair the intestinal injury caused by Muscovy duck reovirus (MDRV) infection in Muscovy ducklings. To examine the effect of HEP on intestine mucosal MDRV immunity and ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.08.130
更新日期:2019-11-01 00:00:00
abstract::Plant glycoproteins, especially allergenic glycoproteins such as pollen allergens, often carry antigenic N-glycans with α1-3 fucose and/or β1-2 xylose residue(s) on the trimannosyl core structure. We previously reported that one of such antigenic free-form N-glycans, Man3Xyl1Fuc1GlcNAc2 (M3FX) suppressed IL-4 producti...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.10.231
更新日期:2020-06-15 00:00:00
abstract::A novel esterase, EstH was cloned, purified and characterized from the marine bacterium Zunongwangia sp. The purified EstH showed optimum activity at 30°C and pH 8.5 with ∼50% of original activity at 0°C. EstH was stable in high salt conditions (0-4.5M NaCl). To improve the characteristics and explore the possibilitie...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.03.016
更新日期:2016-06-01 00:00:00
abstract::Protein misfolding and aggregation has been implicated as the cause of more than 20 diseases in humans such as Alzheimer's and Parkinson's and systemic amyloidosis. Retardation of Aβ- 42 aggregation is considered as a promising and challenging strategy for developing effective therapeutics against Alzheimer's disease....
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.03.001
更新日期:2017-06-01 00:00:00
abstract::Pseudomonas chlororaphis subsp. aurantiaca (DSM 19603) was grown on crude glycerol from biodiesel production to produce a medium-chain-length polyhydroxyalkanoate (mcl-PHA), composed of 3-hydroxydodecanoate (43 ± 1.8 mol%), 3-hydroxydecanoate (29 ± 3.1 mol%), 3-hydroxytetradecanoate (12 ± 0.4 mol%), 3-hydroxyoctanoate...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.09.064
更新日期:2019-02-01 00:00:00
abstract::Targeted drug carrier systems not only prolong the long-term circulation of drugs, but also improve their bioavailability. To obtain a pH/temperature synergistically responsive polymer carrier, temperature and pH-sensitive groups were chemically grafted onto a cassava starch backbone. Secondly, the structure of the po...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.09.248
更新日期:2020-12-15 00:00:00
abstract::N-rich biochars were obtained via pyrolysis treatment of chitosan (a low-cost biopolymer from natural biomasses) at mild conditions (in the 284 °C-540 °C range), thus offering an energy efficient and low carbon footprint synthesis. These low surface area N-doped biochars were morphologically and physicochemically char...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.017
更新日期:2020-12-01 00:00:00
abstract::In this study, three types of crosslinked organic-inorganic hybrid biocomposites, such as chitosan/bentonite, chitosan/titanium oxide, and chitosan/alumina (ChBT, ChTi, and ChAl respectively) were prepared and utilized for the removal of nitrate from water by batch biosorption experiments. Effects of crosslinker dose,...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.01.066
更新日期:2017-04-01 00:00:00
abstract::A new series of substituted tacrine/acridine and tacrine/tacrine dimers with aliphatic or alkylene-thiourea linkers was synthesized and the potential of these compounds as novel human acetylcholinesterase (hAChE) and human butyrylcholinesterase (hBChE) inhibitors with nanomolar inhibition activity was evaluated. The m...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2014.06.064
更新日期:2014-09-01 00:00:00
abstract::The chemical extraction and the characterization of polysaccharides from mucilage (MC), pectin (PC) and total pectic mucilage fraction (TFC) of Opuntia ficus indica cladodes as well as the evaluation of their antioxidant activities was investigated. The FTIR spectroscopic analysis revealed the presence of carboxyl and...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.07.042
更新日期:2016-11-01 00:00:00
abstract::Codonopsis pilosula polysaccharides (CPPS) has been shown to possess a variety of biological activities. In previous study, CPPS was successfully modified to obtain its best selenizing Codonopsis pilosula polysaccharides (sCPPS5). The purpose of this study was to investigate the protective effect of the selenizing der...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.12.025
更新日期:2019-03-15 00:00:00
abstract::The purpose work is devoted to design of a simple, one-pot and green approach for the synthesis of molecularly imprinted polymer to construct a selective sorbent for pipette-tip solid phase extraction of Rhodamine B from chili powder samples and its subsequence separation and quantification by high performance liquid ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.07.196
更新日期:2019-10-15 00:00:00
abstract::Chitosan nanoparticles have many applications, such as gene and drug delivery, due to their biocompatibility. Chitosan nanoparticles are currently produced by dissolution in acetic acid that affects the biocompatibility at acidic pH. Here, we synthesized and characterized chitosan (CS) and ascorbate chitosan (AsCS) na...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.04.055
更新日期:2018-08-01 00:00:00
abstract::Among many biodegradable and biocompatible biopolymers, polyhydroxyalkanoates (PHAs), generated by microorganisms, have highly attracted attention in various fields due to their unique physicochemical properties. So far, various types of progresses have been made in environmental and engineering fields by employing PH...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
doi:10.1016/j.ijbiomac.2019.12.181
更新日期:2020-03-01 00:00:00
abstract::Opuntia is a traditional plant from China with medicinal applications. In this experiment, polysaccharides from Opuntia Milpa Alta (MAPs) were analyzed using gas chromatograph-mass spectrometer (GC-MS) method and result showed that MAPs contained mannose (6.37%), rhamnose (14.94%), xylose (1.99%), arabinose (24.07%), ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2011.06.031
更新日期:2011-11-01 00:00:00
abstract::Nanoparticles are produced by means of polyelectrolyte complexation (PEC) of oppositely charged polycationic chitosan (CH) with polyanionic polysaccharide extracted from Sterculia striata exudates (rhamnogalacturonoglycan (RG)-type polysaccharide). The nanoparticles formed with low-molar-mass CH are larger than those ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.03.070
更新日期:2016-07-01 00:00:00
abstract::The structure of an ulvan obtained by water extraction from green seaweed Ulva lactuca was elucidated by using IR, NMR, SEC-MALL and ESIMS methods. The ulvan was also evaluated for its cytotoxic effects on three human cancer cell lines. The results showed that the ulvan was composed of rhamnose, galactose, xylose, man...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.09.040
更新日期:2016-12-01 00:00:00
abstract::In this study starches isolated from Indian quinoa varieties were examined for physicochemical, morphological, thermal and rheological properties. Among isolated starches V1 showed higher starch yield and lower purity (48.45% and 98.32%) than V2 (41.28 and 98.53%). The amylose content was higher for V1 (12.10%) than V...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.04.027
更新日期:2017-09-01 00:00:00
abstract::The study aims at formulation and optimization brain targeted nanoparticles (NP) of Rivastigmine (RT) to improve its therapeutic potential and to verify its safety profile. The NP were optimized using a two factor three level (3(2)) central composite design aiming to minimize particle size; maximize zeta potential and...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2013.04.024
更新日期:2013-08-01 00:00:00
abstract::This study investigated the effect of Lactobacillus fermentum fermentation treatment on the gastrointestinal digestion and fermentation in vitro of polysaccharides from longan pulp. Polysaccharide isolated from unfermented and fermented longan pulp named LP and LP-F, respectively. The molecular weight of LP-F declined...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.01.059
更新日期:2020-03-15 00:00:00
abstract::In this work, the preparation of novel biocompatible polyurethane (PU) elastomers were carried out using curcumin and 1,4-butanediol (1,4-BDO) via step growth polymerization reaction of hydroxyl terminated polybutadiene (HTPB), toluene diisocyanate (TDI) and chitin to improve the biocompatibility, antibacterial and an...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.11.097
更新日期:2020-07-01 00:00:00
abstract::The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice. Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE)...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.04.209
更新日期:2019-07-15 00:00:00
abstract::In the present study, crude polysaccharides were extracted from Gelidium pacificum Okamura, and further purified to obtain the sulfated polysaccharide with molecular weight of 28,807 Da. Its monosaccharide composition mainly consisted of xylose (7.1%), galactose (59.7%) and galacturonic acid (19.76%). And the sulfate ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.02.043
更新日期:2019-05-15 00:00:00
abstract::Although the fungal ability to bind heavy metals has been known for many years, it was not determined which component is responsible for this process. The aim of the study was to isolate (1 → 3)-α-d-glucans from various fungi, select the most efficient compound in the biosorption of heavy metals, and determine its cha...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.07.036
更新日期:2019-09-15 00:00:00
abstract::Sulfated polysaccharides are known to display activity against enveloped viruses, such as herpes and dengue. The aim of this work was to assess the antiviral activity of botryosphaeran, a fungal exocellular (1 → 3)(1 → 6)-β-d-glucan devoid of sulfate groups, and its chemically sulfonated derivatives, against herpes si...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.07.084
更新日期:2019-10-01 00:00:00
abstract::Hypoxia inducible factor 1 alpha (HIF-1α) is a key regulator of the genes involved in the cellular response to hypoxia. This study aims to determine the HIF-1α gene polymorphism and its association with protein expression in diabetic subjects with and without diabetic foot ulcers (DFU). A total of 529 patients with T2...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.08.011
更新日期:2018-11-01 00:00:00
abstract::Black soldier fly can convert organic wastes into their own functional biological macromolecules i.e. chitin that has great potential for biotechnological, biomedical and cosmetic application. The variation in the physiochemical structure of chitin was proved for several insects during metamorphosis stages whereas, it...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.01.293
更新日期:2020-04-15 00:00:00
abstract::In this paper, a new and facile method for the electrochemical determination of l-tyrosine was designed. First, 3-mercaptopropyl trimethoxysilane-functionalized silica nanoparticles were added to a paper disc. Then, the banana peel tissue and the mediator potassium hexacyanoferrate were dropped onto the paper, respect...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.02.060
更新日期:2018-07-01 00:00:00
abstract::Recent reports suggest influence of anti-cancer anthracyclines on telomere dysfunction and their possible interaction with G-quadruplex (G4) DNA as an alternate pathway to apoptosis. We have investigated interaction of epirubicin and adriamycin with G4 DNA [d-AGGG(TTAGGG)3] comprising human telomeric DNA sequence by s...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.08.109
更新日期:2019-10-15 00:00:00