Demonstration of the adhesive properties of the medium-chain-length polyhydroxyalkanoate produced by Pseudomonas chlororaphis subsp. aurantiaca from glycerol.

Abstract:

:Pseudomonas chlororaphis subsp. aurantiaca (DSM 19603) was grown on crude glycerol from biodiesel production to produce a medium-chain-length polyhydroxyalkanoate (mcl-PHA), composed of 3-hydroxydodecanoate (43 ± 1.8 mol%), 3-hydroxydecanoate (29 ± 3.1 mol%), 3-hydroxytetradecanoate (12 ± 0.4 mol%), 3-hydroxyoctanoate (10 ± 1.5 mol%) and 3-hydroxyhexanoate (6 ± 0.3 mol%). The biopolymer had an average molecular weight of 1.1 × 105 Da, with a polydispersity index of 1.5, and was semi-crystalline, as shown by its crystallinity index of 37 ± 0.2%. It had low melting (44 °C) and glass transition (-48 °C) temperatures, and was thermally stable up to 285 °C. The biopolymer films were elastic and translucid, were hydrophobic and presented relatively high permeability to oxygen and carbon dioxide. The films demonstrated to have good adhesion properties towards porcine skin and human skin. The tension (61.1 ± 20.6 kPa) and shear (12.7 ± 2.14 kPa) bond strength of the mcl-PHA for porcine skin suggest its potential as a biomaterial for the development of novel natural adhesives for wound closure or wound dressings.

journal_name

Int J Biol Macromol

authors

Pereira JR,Araújo D,Marques AC,Neves LA,Grandfils C,Sevrin C,Alves VD,Fortunato E,Reis MAM,Freitas F

doi

10.1016/j.ijbiomac.2018.09.064

subject

Has Abstract

pub_date

2019-02-01 00:00:00

pages

1144-1151

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(18)33650-X

journal_volume

122

pub_type

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