Abstract:
:A protein extract obtained from fig tree (Ficus carica) latex containing ficin activity was immobilized on glyoxyl agarose. Different biocatalyst loadings were used (3, 10, 30 and 85 mg/g). When casein was used as substrate, the expressed activities were 60%, 58%, 41% and 14%, respectively, very likely due to casein diffusional limitations. As expected, an increase of the concentration of either free or immobilized ficin reduced the clotting time of casein solution and milk. However, maintaining the same amount of ficin, lowly loaded ficin biocatalysts were unable to produce the clotting neither of the casein solutions nor of the milk, while highly loaded catalysts produced a good aggregate. Performing the proteolytic milk treatment at 4 °C to prevent aggregation and them incubating the milk at 40 °C, the use of immobilized enzyme in milk clotting gave coagulum yields of 19%, 24% and 27% for the 10 mg/g, 30 mg/g and 85 mg/g immobilized ficin respectively, while free ficin gave a yield of around 20% under similar ficin concentrations.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Siar EH,Morellon-Sterling R,Zidoune MN,Fernandez-Lafuente Rdoi
10.1016/j.ijbiomac.2019.12.140subject
Has Abstractpub_date
2020-02-01 00:00:00pages
419-426eissn
0141-8130issn
1879-0003pii
S0141-8130(19)38868-3journal_volume
144pub_type
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