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Biodeuteration of poly(beta-hydroxybutyrate).

Abstract：

:The formation of poly(beta-hydroxybutyrate), PHB, by Rhodobacter sphaeroides and Alcaligenes eutrophus was studied using the following carbon sources and solvents: (1), acetate in H2O; (2), D3-acetate in H2O; (3), acetate in 90 to 92% D2O; and (4), D3-acetate in 90 to 92% D2O. The growth of Rb. sphaeroides cultured under condition (2) showed no apparent deuterium isotope effect, while considerably slowed growth in the presence of D2O was observed under conditions (3) and (4). In all cases, the PHB produced under deuterium enriched conditions was of high molecular weight. Interestingly, comparatively high volumetric formation of partially deuterated PHB was obtained using culture condition (4) for A. eutrophus. Fourier transform infrared spectroscopy (FT-i.r.), pyrolysis gas chromatography mass spectrometry (PGC-m.s.), and nuclear magnetic resonance (n.m.r.) were used to establish the extent and distribution of deuterium in the PHB samples produced. Partially deuterated PHB was obtained in each case, using a deuterium enriched culture. Considerable differences in the extent and distribution of deuterium were found between micro-organisms and culture conditions.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Gross RA,Ulmer HW,Lenz RW,Tshudy DJ,Uden PC,Brandt H,Fuller RC

doi

10.1016/s0141-8130(05)80017-0

keywords:

subject

Has Abstract

pub_date

1992-02-01 00:00:00

pages

33-40

issue

1

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(05)80017-0

journal_volume

14

pub_type

杂志文章

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