当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Methods for preparation of low abundance glycoproteins from mammalian cell supernatants.

Methods for preparation of low abundance glycoproteins from mammalian cell supernatants.

Abstract：

:Proteins secreted to mammalian cell supernatants are usually in a low concentration and purity, due to the limitation of the expression systems or the presence of a large amount of contaminant proteins from the cell medium. So, initial protein recovery from cell supernatants requires of a highly specific chromatography step. We compared several purification methods based on affinity chromatography for purification of proteins from cell culture supernatants: metal chelate affinity, strep-tag and immunopurification with a monoclonal antibody. Soluble receptor glycoproteins were engineered with the corresponding peptide tag at their C-terminal end. The proteins were expressed in 293T cells and secreted to the cell supernatant, as monitored by sandwich ELISA. Supernatants were run through the different chromatography columns and several purification-related parameters determined. While all column-retained proteins were easily eluted, the chelating and immunopurification chromatography gave the highest yield and the latest method provided a sample with the highest purity. So, in spite of its cost, immunopurification chromatography gave optimal results for purification of a low abundance protein from a cell supernatant. Finally, we applied a protein expression system together with immunopurification chromatography for preparation of a glycoprotein for crystallization.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Ordoño D,Enjuanes L,Casasnovas JM

doi

10.1016/j.ijbiomac.2006.05.010

subject

Has Abstract

pub_date

2006-08-15 00:00:00

pages

151-6

issue

1-3

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(06)00178-4

journal_volume

39

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Transglycosidase activity of glycosynthase-type mutants of a fungal GH20 β-N-acetylhexosaminidase.

    abstract：:β-N-Acetylhexosaminidases (CAZy GH20, EC 3.2.1.52) are exo-glycosidases specific for cleaving N-acetylglucosamine and N-acetylgalactosamine moieties of various substrates. The β-N-acetylhexosaminidase from the filamentous fungus Talaromyces flavus (TfHex), a model enzyme in this study, has a broad substrate flexibilit...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.05.273

    authors： Kapešová J,Petrásková L,Kulik N,Straková Z,Bojarová P,Markošová K,Rebroš M,Křen V,Slámová K

    更新日期：2020-10-15 00:00:00

  • Characteristics of xanthan gum-based biodegradable superporous hydrogel.

    abstract：:A novel biopolymer-based superporous hydrogel (SPH) was synthesized through chemical crosslinking by graft copolymerization of 2-hydroxyethyl methacrylate (HEMA) and acrylic acid (AA) on to xanthan gum (XG) via redox initiator system of ammonium persulfate (APS) and N, N, N', N'-tetramethylethylenediamine (TMED), in t...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2009.07.007

    authors： Gils PS,Ray D,Sahoo PK

    更新日期：2009-11-01 00:00:00

  • Facile immobilization of Bacillus licheniformis γ-glutamyltranspeptidase onto graphene oxide nanosheets and its application to the biocatalytic synthesis of γ-l-glutamyl peptides.

    abstract：:For the practical application of Bacillus licheniformis γ-glutamyltranspeptidase (BlGGT), we illustrated a simple and efficient approach to fabricate a biocatalytic system by immobilizing the enzyme onto graphene oxide (GO) nanosheets via both non-covalent (GO-BlGGT) and covalent (GO/GA-BlGGT) bonds. The enzyme-loadin...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.11.153

    authors： Lin LL,Chi MC,Lan YJ,Lin MG,Juang TY,Wang TF

    更新日期：2018-10-01 00:00:00

  • Purification, biochemical and biophysical characterization of lysosomal β-D-glucuronidase from an edible freshwater mussel, Lamellidens corrianus.

    abstract：:A lysosomal glycosidase, β-glucuronidase, has been purified to homogeneity, from the soluble extracts of a freshwater mussel, L. corrianus, by a series of chromatography techniques involving phenyl-Sepharose, ion exchange, affinity and gel filtration chromatography. In native PAGE, β-glucuronidase resolved into a sing...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.02.190

    authors： Konada RSR,Venugopal A,Nadimpalli SK

    更新日期：2020-06-01 00:00:00

  • Chitosan-coated electrospun PLA fibers for rapid mineralization of calcium phosphate.

    abstract：:In this work, hydroxyapatite (HA) mineralized on chitosan (CS)-coated poly(lactic acid) (PLA) nanofiber mat was prepared and compared in terms of mineralization characteristics. Significant calcium phosphate crystals formed on various concentrations of CS-coated PLA fiber mat with better uniformity after 2h of incubat...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.04.039

    authors： Lin CC,Fu SJ,Lin YC,Yang IK,Gu Y

    更新日期：2014-07-01 00:00:00

  • Ginger polysaccharides induced cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.

    abstract：:In this study, ginger polysaccharide (GP) was obtained from ginger by enzymatic method, its chemical properties and antitumor activity were investigated. The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.10.169

    authors： Wang Y,Wang S,Song R,Cai J,Xu J,Tang X,Li N

    更新日期：2019-02-15 00:00:00

  • Structural and morphological evaluation of CNC reinforced PVA/Starch biodegradable films.

    abstract：:Nanocomposite films containing cellulose nanocrystals (CNC), as the reinforcing phase, and poly(vinyl alcohol) (PVA)/starch (S) blend, as the matrix, were prepared by solution casting technique. For the preparation, 5, 10, 15 wt% CNC suspension were incorporated in blends containing 90 wt% PVA/10 wt% S, 75 wt% PVA/25 ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.05.036

    authors： Popescu MC,Dogaru BI,Goanta M,Timpu D

    更新日期：2018-09-01 00:00:00

  • Tuning the microstructure and electrochemical behavior of lignin-based ultrafine carbon fibers via hydrogen-bonding interaction.

    abstract：:Hardwood Kraft lignin (HKL)-based ultrafine carbon fibers with different pore structures and properties were prepared by controlling the intermolecular interaction between HKL and incorporated poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) (PEG-PPG-PEG) triblock copolymer. The thermal p...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.11.235

    authors： Wang S,Innocent MT,Chen J,Wang Q,Ma W,Tang J

    更新日期：2020-08-15 00:00:00

  • Optimized purification process of polysaccharides from Carex meyeriana Kunth by macroporous resin, its characterization and immunomodulatory activity.

    abstract：:The analytical hierarchy process (AHP) combined with response surface methodology (RSM) was used to optimize the purification conditions of crude polysaccharides from Carex meyeriana Kunth (CMKP) by macroporous resin. The optimal purification conditions as follows: elution volume of 2.74 BV, flow rate of 1.88 BV/h, an...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.03.207

    authors： Hu Z,Zhou H,Li Y,Wu M,Yu M,Sun X

    更新日期：2019-07-01 00:00:00

  • On the relationship between the conserved 'black' and 'gray' structural clusters and intrinsic disorder in parvalbumins.

    abstract：:Recently we found two highly conserved structural motifs in the members of the EF-hand protein family, which provide a supporting scaffold for their Ca2+ binding loops. Each structural motif is formed by a cluster of three amino acids. These clusters were called 'black' cluster (cluster I) and 'gray' cluster (cluster ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.08.183

    authors： Deryusheva EI,Denesyuk AI,Denessiouk K,Uversky VN,Permyakov SE,Permyakov EA

    更新日期：2018-12-01 00:00:00

  • Digestion kinetics of native and modified cardaba banana starch: A biphasic approach.

    abstract：:To understand the mechanism through which cardaba banana starch is hydrolysed, the starch digestion kinetics of native and modified cardaba banana starch samples of Nigeria origin were investigated using an in vitro procedure. The digestion kinetics of the starch samples revealed the samples exhibited a biphasic diges...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.03.089

    authors： Olawoye B,Gbadamosi SO

    更新日期：2020-07-01 00:00:00

  • Oligosaccharide from apple induces apoptosis and cell cycle arrest in HT29 human colon cancer cells.

    abstract：:It is reported that apple polysaccharide can prevent colon cancer growth and impede colon cancer progression. Apple oligosaccharide was prepared by the combination of alkaline hydrolysis and enzymolysis of apple polysaccharides, and purified by anion column chromatography. The aim of this study is to explore the effec...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.03.034

    authors： Li Q,Zhou S,Jing J,Yang T,Duan S,Wang Z,Mei Q,Liu L

    更新日期：2013-06-01 00:00:00

  • Characterization, antioxidant activity and immunomodulatory activity of polysaccharides from the swollen culms of Zizania latifolia.

    abstract：:The swollen culms of Zizania latifolia have been used as a vegetable and traditional herbal medicine in China, Japan, Korea and Southeast Asia countries. Up to date, there is little information about the polysaccharides from the swollen culms of Zizania latifolia and their potential bioactivities. In the present study...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.12.010

    authors： Wang M,Zhu P,Zhao S,Nie C,Wang N,Du X,Zhou Y

    更新日期：2017-02-01 00:00:00

  • Characterization of a novel exopolysaccharide produced by Lactobacillus gasseri FR4 and demonstration of its in vitro biological properties.

    abstract：:In this study, hetero-exopolysaccharide of Lactobacillus gasseri FR4 isolated from native chicken was produced and purified. The molecular weight of LgEPS was found to be 1.86×105Da. The gas chromatographic analysis revealed that the LgEPS was majorly composed of glucose (65.31%), mannose (16.51%), galactose (8.45%), ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.11.062

    authors： Rani RP,Anandharaj M,David Ravindran A

    更新日期：2018-04-01 00:00:00

  • Physicochemical properties and biological activities of novel blend films using oxidized pectin/chitosan.

    abstract：:Pectin has been widely used in a variety of biomedical applications. In this study, it was modified with sodium periodate as an oxidant and characterized by physicochemical methods Periodate oxidation increased the contents of dialdehyde units and carboxyl groups in pectin, and a decrease in pectin viscosity was measu...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.01.018

    authors： Chetouani A,Follain N,Marais S,Rihouey C,Elkolli M,Bounekhel M,Benachour D,Le Cerf D

    更新日期：2017-04-01 00:00:00

  • Effect of organic solvent on enzymatic degradation of cyclic PBS-based polymers by lipase N435.

    abstract：:Poly(butylene succinate-co-cyclohexane dimethanol succinate) (P(BS-co-CHDMS)) and poly(butylene succinate-co-butanediol cyclohexanedicarboxylic acid) (P(BS-co-BCHDA)) were catalytically degraded by Candida antarctica lipase Novozyme 435 (N435) in CHCl3 and THF. The results indicated that the degradation rate was P(BS-...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.06.216

    authors： Li CT,Zhang M,Weng YX,Zhao D

    更新日期：2019-09-15 00:00:00

  • Chitosan-alginate immobilized lipase based catalytic constructs: Development, characterization and potential applications.

    abstract：:In this study, lipase (LIP) was isolated from Aspergillus crevinus, statistically optimized and purified via ammonium sulfate fractionation (ASF), and Sephadex G-100 gel permeation chromatography. LIP was 2.26-folds purified with a specific activity of 223.60 U/mg. The molecular mass was estimated to be 60 kDa on sodi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.07.192

    authors： Rashid R,Anwar Z,Zafar M,Rashid T,Butt I

    更新日期：2018-11-01 00:00:00

  • Polysaccharide isolated from Sarcodon aspratus induces RAW264.7 activity via TLR4-mediated NF-κB and MAPK signaling pathways.

    abstract：:Our previous report showed that the novel polysaccharide SAP isolated from the fruiting bodies of Sarcodon aspratus induced Hela cells apoptosis via mitochondrial dysfunction. In this study we found that SAP enhanced immunostimulatory activities of RAW264.7 cells, which was characterized by increased the production of...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.08.147

    authors： Wang DD,Pan WJ,Mehmood S,Cheng XD,Chen Y

    更新日期：2018-12-01 00:00:00

  • Characterization of films based on chitosan lactate and its blends with oxidized starch and gelatin.

    abstract：:Minimal inhibitory concentration (MIC) of chitosan lactate (CHL) was tested against bacteria and phytopathogenic fungi. Then, the structural, physicochemical and antimicrobial properties of films based on CHL, oxidized potato starch (OPS), and gelatin (GEL) were investigated. With the exception of Rhizopus nigricans, ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.03.032

    authors： Kowalczyk D,Kordowska-Wiater M,Nowak J,Baraniak B

    更新日期：2015-01-01 00:00:00

  • A polysaccharide from Hedyotis diffusa interrupts metastatic potential of lung adenocarcinoma A549 cells by inhibiting EMT via EGFR/Akt/ERK signaling pathways.

    abstract：:In this study we investigated the potential effects of a polysaccharide (HDP) from Hedyotis diffusa on the metastasis in human lung adenocarcinoma A549 cells. HDP (25, 50 and 100μg/ml) significantly suppressed the cell adhesion, invasion and migration of A549 cells in a dose dependent manner by downregulation of matri...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.02.040

    authors： Lin L,Cheng K,He Z,Lin Q,Huang Y,Chen C,Xie Z,Chen L,Liang Z

    更新日期：2019-05-15 00:00:00

  • Characterization, in vitro binding properties, and inhibitory activity on pancreatic lipase of β-glucans from different Qingke (Tibetan hulless barley) cultivars.

    abstract：:In order to explore Qingke β-glucans as functional food ingredients for prevention of obesity, the physicochemical structures, in vitro binding properties, and inhibitory activities on pancreatic lipase of β-glucans from three different Qingke cultivars, including Ganyucang (black), Dingqing (blue), and Zangqing 320 (...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.09.023

    authors： Guo H,Lin S,Lu M,Gong JDB,Wang L,Zhang Q,Lin DR,Qin W,Wu DT

    更新日期：2018-12-01 00:00:00

  • Chitosan combined with swimming promotes health in rats.

    abstract：PURPOSE:Chitosan is an easily accessible and biocompatible natural molecule which facilitate the immune system. In recent studies, chitosan is being applied to the drug nanosphere to deliver drugs. However, whether chitosan could promote health under exercising condition remains yet to be elucidated. Hence, we designed...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.07.067

    authors： Xiong Y,Li X,Xiong M,Vikash S,Liu P,Wang M,Zhu Y,Yuan W,Zhang Q,Fu B,Qin C

    更新日期：2018-10-15 00:00:00

  • Oxidative stress level and dehydrin gene expression pattern differentiate two contrasting cucumber F1 hybrids under high fertigation treatment.

    abstract：:Two cucumber F1 cultivar hybrids were investigated for stress tolerance markers upon application of different strength of Hoagland fertigation solutions (HG). 'Joker' and 'Oitol' cultivar hybrids were studied, representing typically field grown and greenhouse cultivated genotypes, respectively. At standard fertigation...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.06.050

    authors： Oszlányi R,Mirmazloum I,Pónya Z,Szegő A,Jamal S,Bat-Erdene O,Papp I

    更新日期：2020-10-15 00:00:00

  • Incorporating allylated lignin-derivatives in thiol-ene gel-polymer electrolytes.

    abstract：:Growing environmental and economic concerns as well as the uncertainty that accompanies finite petrochemical resources contributes to the increase in research and development of bio-based, renewable polymers. Concurrently, industrial and consumer demand for smaller, safer, and more flexible technologies motivates a gl...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.02.160

    authors： Baroncini EA,Stanzione JF 3rd

    更新日期：2018-07-01 00:00:00

  • Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage.

    abstract：:This study was designed to study the chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage. A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fo...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.10.055

    authors： Xiong L,Ouyang KH,Jiang Y,Yang ZW,Hu WB,Chen H,Wang N,Liu X,Wang WJ

    更新日期：2018-02-01 00:00:00

  • Preparation of self-assembled silk sericin nanoparticles.

    abstract：:Silk sericin (SS) possessing moisture-retaining property was reacted with activated poly(ethylene glycol) (PEG) to obtain self-assembled SS nanoparticles. The aliphatic and aromatic hydroxyl groups of serine and tyrosine residues as the reaction sites in SS were clarified by amino acid analysis and 1H NMR spectroscopy...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/s0141-8130(03)00023-0

    authors： Cho KY,Moon JY,Lee YW,Lee KG,Yeo JH,Kweon HY,Kim KH,Cho CS

    更新日期：2003-03-01 00:00:00

  • Study of the mechanical degradation mechanism of guar gum in turbulent flow by FTIR.

    abstract：:Guar gum is used in low concentrations as a drag reducing agent in turbulent flows to significantly accelerate flow in oil pipelines, oil well operations and aqueous systems. Drag reduction also promotes a decrease in energy demand in pumping systems. However, the polymers undergo mechanical degradation and lose the a...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.09.214

    authors： Motta MVL,de Castro EVR,Muri EJB,Costalonga ML,Loureiro BV,Filgueiras PR

    更新日期：2019-01-01 00:00:00

  • Mastoid obliteration using 3D PCL scaffold in combination with alginate and rhBMP-2.

    abstract：:Various materials, both biological and alloplastic, have been used for mastoid obliteration after canal wall-down mastoidectomy in chronic otitis media. The purpose of this study is to investigate the osteoinductive potential of PCL scaffolds in mastoid obliteration, and to test that osteoinductivity can be enhanced b...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.10.011

    authors： Jang CH,Kim MS,Cho YB,Jang YS,Kim GH

    更新日期：2013-11-01 00:00:00

  • Characterization and application of keratinolytic paptidases from Bacillus spp.

    abstract：:Solid keratin-rich waste management is one of essential research area in nowadays. Conventional chemical and high thermal keratin waste decomposition methods are fully explored and not enough effective for future biotechnology perspectives. However, traditional keratin-rich waste decomposition methods could be replace...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.03.046

    authors： Gegeckas A,Šimkutė A,Gudiukaitė R,Čitavičius DJ

    更新日期：2018-07-01 00:00:00

  • Description of a new surface morphology for chitin extracted from wings of cockroach (Periplaneta americana).

    abstract：:In this study a new morphology of chitin, which could find wide applications in the fields of medicine, pharmacy, agriculture, food and textiles, has been described. The chitin was isolated from the wings of Periplaneta americana employing a conventional method. Considering chitin isolation studies conducted previousl...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.01.015

    authors： Kaya M,Baran T

    更新日期：2015-04-01 00:00:00