Abstract：
:A thermostable superoxide dismutase from thermophilic bacterium Anoxybacillus gonensis KA 55 MTCC 12684 which was isolated from Manikaran hotspring of Himachal Pradesh was purified to apparent homogeneity by fractional ammonium sulphate precipitation and anion exchange chromatography. A purification factor of 33.1-fold was achieved, with the purified enzyme exhibiting specific activity of 5758.4 U/mg protein. The purified superoxide dismutase was optimally active at pH 9.0 and displayed stability over a broad pH range of 7.0-10.0 and was stable up to 70 °C. SOD was localized in polyacrylamide gel by activity staining, based on the reduction of nitroblue tetrazolium (NBT) by superoxide ion. The molecular weight of superoxide dismutase was calculated as 31 kDa by SDS-PAGE. The Km and Vmax values of purified enzyme were found to be 1.002 mM and 14,285.71 U/mg of protein respectively. Tests of inhibitors indicated that the enzyme activity was inhibited by hydrogen peroxide and potassium cyanide but not by sodium azide showing that purified SOD was Cu/ZnSod.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Bhatia K,Mal G,Bhar R,Jyoti,Attri C,Seth Adoi
10.1016/j.ijbiomac.2018.06.031subject
Has Abstractpub_date
2018-10-01 00:00:00pages
1133-1139eissn
0141-8130issn
1879-0003pii
S0141-8130(18)31910-Xjournal_volume
117pub_type
杂志文章abstract：:The aim of this study was to use micro-encapsulation technology to create microcapsules containing anthocyanins from chokeberry with guar gum, gum arabic, pectin, β-glucan and inulin as wall material. Aqueous extracts from chokeberry fruit were enclosed and spray dried using maltodextrin as a coating material with the...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.02.073
更新日期：2019-05-15 00:00:00
abstract：:In this work, we prepared novel cellulosic paper by incorporating tannin into the kraft pulp for potential application in active food packaging. The kraft pulp fibers were firstly periodate oxidized to obtain the dialdehyde cellulosic fibers, and then reacted with varied dosages of tannin to incorporate them into the ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.06.101
更新日期：2020-11-01 00:00:00
abstract：:Time-dependent intermolecular sulphydryl-disulphide interchange involving beta-lactoglobulin adsorbed at the oil-water interface in n-tetradecane-in-water emulsions (10 wt% oil, 0.5 wt% protein, pH 7.0) has been investigated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). While only monome...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(91)90006-g
更新日期：1991-02-01 00:00:00
abstract：:An alkaline serine protease from a newly isolated salt-tolerant alkaliphilic actinomycetes, Brachystreptospora xinjiangensis OM-6 was purified with 35- and 26-fold purification and 47% and 22% yield employing two steps and one step methods, respectively. The enzyme was quite stable at 80 °C in 30% Na-glutamate with th...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.01.028
更新日期：2013-05-01 00:00:00
abstract：:The effect of putrescine on the dynamics, conformation, and kinetics of acid phosphatase investigated via different experimental and theoretical methods. The Stern-Volmer constants (Ksv) for the acid phosphatase- putrescine compound was obtained at different temperatures. Therefore, putrescine quenched the intensity o...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.02.057
更新日期：2020-05-01 00:00:00
abstract：:Present communication reports on the synthesis and characterization of Hg(II) sorbing millimeter sized porous titania spheres (TSP). The synthesis utilizes guar gum-alginate hybrid beads as sacrificial template to polymerize titanium(IV) isopropoxide. The hybrid beads are crafted by pouring guar-alginate mixed solutio...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.08.016
更新日期：2015-01-01 00:00:00
abstract：:In this study a new morphology of chitin, which could find wide applications in the fields of medicine, pharmacy, agriculture, food and textiles, has been described. The chitin was isolated from the wings of Periplaneta americana employing a conventional method. Considering chitin isolation studies conducted previousl...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.01.015
更新日期：2015-04-01 00:00:00
abstract：:Acetaminophen (APAP)-induced acute kidney injury (AKI) has turned into a typical reason for clinic obtained renal disappointment. In any case, the advancement of prophylaxis procedures and endorsed treatments for APAP-AKI is restricted. Sika deer antler protein (SDAPR) has a protective effect on drug-induced AKI. The ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.08.164
更新日期：2019-12-01 00:00:00
abstract：:Coriolus versicolor is an edible medicinal mushroom in China. Two polysaccharides, named as CVPn and CVPa were separated from the dried fruiting bodies of Coriolus versicolor by water extraction and ethanol precipitation. Their chemical structures were well elucidated with overall consideration of monosaccharide compo...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.11.018
更新日期：2021-01-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.07.013
更新日期：2015-11-01 00:00:00
abstract：:Synthesis of Starch-g-(Poly N, N-dimethylacrylamide-co-acrylic acid) was carried out by solution polymerization technique using potassium perdisulfate (K(2)S(2)O(8)) as the initiator. The graft copolymer was characterized by measuring molecular weight, using size exclusion chromatography (SEC), FTIR spectroscopy and X...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.09.003
更新日期：2015-01-01 00:00:00
abstract：:Survival of the Acinetobacter baumannii inside host requires different micronutrients such as iron, but their bioavailability is limited because of nutritional immunity created by host. A. baumannii has to develop mechanisms to acquire nutrient iron during infection. The present study is an attempt to identify membran...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.12.173
更新日期：2019-03-15 00:00:00
abstract：:The cross-linked enzyme aggregates (CLEAs) involves formation of a number of covalent bonds between enzyme and the matrix using glutaraldehyde. In general, amino groups of lysine, sulfhydryl groups of cysteine, phenolic OH groups of tyrosine, or imidazol group of histidine are used for enzyme binding under mild condit...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.07.075
更新日期：2018-10-15 00:00:00
abstract：:This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2006.02.001
更新日期：2006-03-22 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.10.223
更新日期：2020-06-01 00:00:00
abstract：:The major problems of porphyrins as promising materials for photodynamic therapy (PDT) are their low solubility, subsequently aggregation in biological environments, and a lack of tumor selectivity. With this in mind, a chitosan-based hydrogel conjugated with tetrakis(4-aminophenyl)porphyrin (NH2-TPP) and 2,4,6-tris(p...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.12.169
更新日期：2018-04-15 00:00:00
abstract：:Enzymatically-active bacterial cellulose (BC) was prepared by non-covalent immobilization of a hybrid enzyme composed by a β-galactosidase from Thermotoga maritima (TmLac) and a carbohydrate binding module (CBM2) from Pyrococcus furiosus. TmLac-CBM2 protein was bound to BC, with higher affinity at pH 6.5 than at pH 8....
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.04.081
更新日期：2018-08-01 00:00:00
abstract：:Crosslinked chitosan beads were grafted with N-vinyl-2-pyrrolidone (NVP) using ammonium persulfate (APS) as free radical initiator. Important variables on graft copolymerization such as temperature, reaction time, concentration of initiator and concentration of monomer were optimized. The results revealed optimum cond...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.09.061
更新日期：2018-02-01 00:00:00
abstract：:Corn starch was melt-blended with glycerol (70/30) to prepare thermoplastic starch (TPS) at 140 °C for 10 min. The TPS was melt-blended with polyethylene grafted with maleic anhydride (PE-MAH) and carboxymethyl cellulose (CMC) to improve the properties of the TPS. Phase determination analysis and SEM images demonstrat...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.08.076
更新日期：2018-12-01 00:00:00
abstract：:Chitosan was chemically modified through a four-step procedure. First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic a...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2020-05-15 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(91)90045-v
更新日期：1991-06-01 00:00:00
abstract：:The potency of VEGF-based anti-angiogenic strategies in cancer therapy and the brilliant characteristics of VHHs motivated us to directly block VEGF binding to its receptor with neutralizing single domain antibodies, thereby fading away the VEGF signaling pathway. Considering with high resolution crystal structure of ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.02.047
更新日期：2015-01-01 00:00:00
abstract：:This study reports on the exploitation of keratinous hydrolysate by keratinase enzymes to produce vitamin B-complex. Toward this end, keratinase enzyme was produced by Bacillus thuringiensis strain MT1, newly isolated from cattle-yard utilising donkey hairs. Scanning electron microscope (SEM) and Fourier transform inf...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.03.032
更新日期：2020-06-15 00:00:00
abstract：:The chitosan chains were integrated with MCM-48 mesoporous silica in an eco-friendly composite (CH/MCM-48) of enhanced adsorption capacity. The prepared CH/MCM-48 composite was applied in systematic retention of U (VI) as well as Sr (II) ions from water as the commonly detected radioactive pollutants. It displayed pro...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2021.01.136
更新日期：2021-01-23 00:00:00
abstract：:Proteins secreted to mammalian cell supernatants are usually in a low concentration and purity, due to the limitation of the expression systems or the presence of a large amount of contaminant proteins from the cell medium. So, initial protein recovery from cell supernatants requires of a highly specific chromatograph...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2006.05.010
更新日期：2006-08-15 00:00:00
abstract：:Azadirachta indica leaf is used by Indian population for the healing of various diseases including viral infection. Herein, we analyzed the antiherpetic (HSV-1) activity of two polysaccharides (P1 and P2) isolated from the leaf of A. indica and their chemically sulfated derivatives (P1S and P2S). The molecular weights...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.06.129
更新日期：2019-09-15 00:00:00
abstract：:The aim of this study was to develop a new bioactive gelatin-oxidized starch nanofibers containing Lawsonia Inermis (henna) for treating second-degree burn wounds. Continuous, smooth, and bead-free fibers were obtained when the gelatin-starch ratio was 70/30.Additionally, the fiber dimeter was reduced with increasing ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.10.061
更新日期：2018-02-01 00:00:00
abstract：:Heparosan polysaccharide, known as a heparin precursor, can be used in drug delivery systems due to its good biocompatibility and anti-cancer effect. But few studies on the cellular uptake mechanism of heparosan polysaccharide-based nanocarrier have been investigated. Therefore, the intracellular trafficking and the u...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.02.133
更新日期：2019-06-01 00:00:00
abstract：:Amygdalin, despite possessing anticancerous properties, has been viewed as a controversial choice due to the presence of the cyanide group. Here, we synthesise and investigate the potential of alginate-chitosan nanoparticles (ACNPs) as drug delivery agents for amygdalin encapsulation and its delivery to cancer cells. ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.02.191
更新日期：2020-06-15 00:00:00
abstract：:In this paper, selenized Artemisia sphaerocephala polysaccharides (SePAS) were obtained through employing N-methyl-2-pyrrolidone hydrosulfate as catalyst, which showed a maximum Se content enhanced to 8744 μg/g. FT-IR, 1D/2D NMR, X-ray photoelectron spectroscopy (XPS) and size-exclusion chromatograph analysis exhibite...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.05.261
更新日期：2020-10-01 00:00:00
