当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Biochemical characterisation and application of keratinase from Bacillus thuringiensis MT1 to enable valorisation of hair wastes through biosynthesis of vitamin B-complex.

Biochemical characterisation and application of keratinase from Bacillus thuringiensis MT1 to enable valorisation of hair wastes through biosynthesis of vitamin B-complex.

Abstract：

:This study reports on the exploitation of keratinous hydrolysate by keratinase enzymes to produce vitamin B-complex. Toward this end, keratinase enzyme was produced by Bacillus thuringiensis strain MT1, newly isolated from cattle-yard utilising donkey hairs. Scanning electron microscope (SEM) and Fourier transform infrared spectrophotometer (FTIR) analyses demonstrated hairs disintegration and the disruption of the disulphide bonds of the keratin structure, respectively. The biochemical characterisation of the produced enzyme exhibited optimal activity of 422 U/ml at 50 °C and pH 9 with a molecular mass of 80 kDa. The enzyme activity was entirely deactivated by Ethylenediaminetetraacetic acid (EDTA), implying the existence of a metallokeratinase group. Donkey hairs were thus treated with metallokeratinase, emancipating eight essential and eight more non-essential amino acids, which were identified employing amino acid analyser. These amino acids were subsequently utilised by Saccharomyces cerevisiae strain ATCC 64712, at different concentrations, to produce vitamin B-complex. High-performance liquid chromatography (HPLC) analysis revealed the synthesis of vitamins B1, B2, and B12 at various levels associated with concentrations of supplemented amino acids. This report thus highlights the feasible application of keratinase enzyme as an eco-friendly approach to managing hair waste, and concurrently promotes the implementation of hair-based hydrolysate in vitamin B-complex biosynthesis.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Hassan MA,Taha TH,Hamad GM,Hashem M,Alamri S,Mostafa YS

doi

10.1016/j.ijbiomac.2020.03.032

subject

Has Abstract

pub_date

2020-06-15 00:00:00

pages

561-572

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(19)39521-2

journal_volume

153

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Kinetic and mechanistic considerations in the gelation of genipin-crosslinked gelatin.

    abstract：:The gelling properties (gel time (t(gel)) and gel strength) of a 10% (w/w) gelatin sol were investigated as a function of genipin (GP) concentration (0-15 mM) and temperature (25-55 degrees C) to discern mechanisms and optimal conditions for fixation. Gel time increased with increasing temperature, reached a maximum, ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2006.04.010

    authors： Nickerson MT,Patel J,Heyd DV,Rousseau D,Paulson AT

    更新日期：2006-11-15 00:00:00

  • Revealing of sugar utilization systems in Halomonas sp. YLGW01 and application for poly(3-hydroxybutyrate) production with low-cost medium and easy recovery.

    abstract：:Poly(3-hydroxybutyrate) (PHB) is a common polyhydroxyalkanoate (PHA) with potential as an alternative for petroleum-based plastics. Previously, we reported a new strain, Halomonas sp. YLGW01, which hyperproduces PHB with 94% yield using fructose. In this study, we examined the PHB production machinery of Halomonas sp....

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.11.163

    authors： Park YL,Song HS,Choi TR,Lee SM,Park SL,Lee HS,Kim HJ,Bhatia SK,Gurav R,Park K,Yang YH

    更新日期：2021-01-15 00:00:00

  • Chitosan nanocomposite films based on Ag-NP and Au-NP biosynthesis by Bacillus Subtilis as packaging materials.

    abstract：:Chitosan-silver (CS-Ag) and Chitosan-gold (CS-Au) nanocomposites films were synthesized by a simple chemical method. A local bacterial isolate identified as Bacillus subtilis ss subtilis was found to be capable to synthesize both silver nanoparticles (Ag-NP) and gold nanoparticles (Au-NP) from silver nitrate (AgNO3) a...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.05.047

    authors： Youssef AM,Abdel-Aziz MS,El-Sayed SM

    更新日期：2014-08-01 00:00:00

  • Enhancing the thermostability of Rhizopus chinensis lipase by rational design and MD simulations.

    abstract：:To improve the thermostability of r27RCL from Rhizopus chinensis and broaden its industrial applications, we used rational design (FoldX) according to ΔΔG calculation to predict mutations. Four thermostable variants S142A, D217V, Q239F, and S250Y were screened out and then combined together to generate a quadruple-mut...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.05.243

    authors： Wang R,Wang S,Xu Y,Yu X

    更新日期：2020-10-01 00:00:00

  • Stabilization of collagen using plant polyphenol: role of catechin.

    abstract：:Collagen, a unique connective tissue protein finds extensive application as biocompatible biomaterial in wound healing, as drug carriers, cosmetics, etc. A work has been undertaken to study the stabilization of type I collagen using the plant polyphenol catechin. Catechin treated collagen fibres showed a shrinkage tem...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2005.08.005

    authors： Madhan B,Subramanian V,Rao JR,Nair BU,Ramasami T

    更新日期：2005-10-30 00:00:00

  • Comparative analysis reveals the expansion of mitochondrial DNA control region containing unusually high G-C tandem repeat arrays in Nasonia vitripennis.

    abstract：:Insect mitochondrial DNA (mtDNA) ranges from 14 to 19 kbp, and the size difference is attributed to the AT-rich control region. Jewel wasps have a parasitoid lifestyle, which may affect mitochondria function and evolution. We sequenced, assembled, and annotated mitochondrial genomes in Nasonia and outgroup species. Ge...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.11.007

    authors： Lin ZJ,Wang X,Wang J,Tan Y,Tang X,Werren JH,Zhang D,Wang X

    更新日期：2021-01-01 00:00:00

  • Can green solvents be alternatives for thermal stabilization of collagen?

    abstract：:"Go Green" campaign is gaining light for various industrial applications where water consumption needs to be reduced. To resolve this, industries have adopted usage of green, organic solvents, as an alternative to water. For leather making, tanning industry consumes gallons of water. Therefore, for adopting green solv...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.05.021

    authors： Mehta A,Rao JR,Fathima NN

    更新日期：2014-08-01 00:00:00

  • Effects of cataract-causing mutations W59C and W151C on βB2-crystallin structure, stability and folding.

    abstract：:β/γ-Crystallins, the predominant structural proteins in vertebrate lens with lifelong stability to maintain lens transparency, share a high similarity in their primary sequences and tertiary structures. Four conserved Trp residues have been shown to be important to γ-crystallin structure, stability and protection agai...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.05.109

    authors： Zhao WJ,Xu J,Chen XJ,Liu HH,Yao K,Yan YB

    更新日期：2017-10-01 00:00:00

  • Inhibition of tau aggregation and associated cytotoxicity on neuron-like cells by calycosin.

    abstract：:In this study, the in vitro assembly of tau and anti-amyloidogenic properties of one naturally occurring phytoestrogen, calycosin, was investigated by spectroscopic techniques including ThT and ANS fluorescence, CD, Congo red absorbance as well as TEM analysis. Afterwards the cytotoxicity of different amyloid species ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.12.030

    authors： Zhenxia Z,Min L,Peikui Y,Zikai C,Yaqun L,Junli W,Fenlian Y,Yuzhong Z

    更新日期：2020-12-07 00:00:00

  • Thermal, mechanical, and physical properties of seaweed/sugar palm fibre reinforced thermoplastic sugar palm Starch/Agar hybrid composites.

    abstract：:The aim of this research is to investigate the effect of sugar palm fibre (SPF) on the mechanical, thermal and physical properties of seaweed/thermoplastic sugar palm starch agar (TPSA) composites. Hybridized seaweed/SPF filler at weight ratio of 25:75, 50:50 and 75:25 were prepared using TPSA as a matrix. Mechanical,...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.01.079

    authors： Jumaidin R,Sapuan SM,Jawaid M,Ishak MR,Sahari J

    更新日期：2017-04-01 00:00:00

  • Effect of temperature-cycled retrogradation on in vitro digestibility and structural characteristics of waxy potato starch.

    abstract：:The effects of temperature-cycled retrogradation treatment on the structural characteristics and in vitro digestibility of waxy potato starch were investigated in this study. The results showed that the maximum yield of slowly digestible starch (SDS) in waxy potato starch reached 38.63% by retrogradation treatment und...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.03.007

    authors： Xie YY,Hu XP,Jin ZY,Xu XM,Chen HQ

    更新日期：2014-06-01 00:00:00

  • Improved thermal-stability and mechanical properties of type I collagen by crosslinking with casein, keratin and soy protein isolate using transglutaminase.

    abstract：:The inferior thermal- stability of collagen hinders its extensive application in food industry, including edible packaging. To improve the thermal- stability and mechanical properties of collagen, we attempted to crosslink collagen with some proteins possessing excellent thermal stability (i. e., casein, keratin and s...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.01.127

    authors： Wu X,Liu Y,Liu A,Wang W

    更新日期：2017-05-01 00:00:00

  • A polysaccharide from Hedyotis diffusa interrupts metastatic potential of lung adenocarcinoma A549 cells by inhibiting EMT via EGFR/Akt/ERK signaling pathways.

    abstract：:In this study we investigated the potential effects of a polysaccharide (HDP) from Hedyotis diffusa on the metastasis in human lung adenocarcinoma A549 cells. HDP (25, 50 and 100μg/ml) significantly suppressed the cell adhesion, invasion and migration of A549 cells in a dose dependent manner by downregulation of matri...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.02.040

    authors： Lin L,Cheng K,He Z,Lin Q,Huang Y,Chen C,Xie Z,Chen L,Liang Z

    更新日期：2019-05-15 00:00:00

  • Synthesis of chitosan based molecularly imprinted polymer for pipette-tip solid phase extraction of Rhodamine B from chili powder samples.

    abstract：:The purpose work is devoted to design of a simple, one-pot and green approach for the synthesis of molecularly imprinted polymer to construct a selective sorbent for pipette-tip solid phase extraction of Rhodamine B from chili powder samples and its subsequence separation and quantification by high performance liquid ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.07.196

    authors： Bagheri AR,Ghaedi M

    更新日期：2019-10-15 00:00:00

  • Cloning, expression and characterization of β- and γ‑carbonic anhydrase from Bacillus sp. SS105 for biomimetic sequestration of CO2.

    abstract：:Bacterium Bacillus sp. SS105, isolated from Free Air CO2 Enriched (FACE) soil was previously screened for carbonic anhydrase activity and CO2 sequestration. In this study, strain was selected to amplify carbonic anhydrase encoding genes. The CA genes from Bacillus sp. SS105 were found to be homologous with beta‑carbon...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.03.082

    authors： Maheshwari N,Kumar M,Thakur IS,Srivastava S

    更新日期：2019-06-15 00:00:00

  • Injectable pullulan hydrogel for the prevention of postoperative tissue adhesion.

    abstract：:Methods for reducing and preventing postoperative abdominal adhesions have been researched for decades; however, despite these efforts, the formation of postoperative peritoneal adhesions is continuously reported. Adhesions cause serious complications such as postoperative pain, intestinal obstruction, and infertility...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.02.026

    authors： Bang S,Lee E,Ko YG,Kim WI,Kwon OH

    更新日期：2016-06-01 00:00:00

  • Poly(vinyl chloride) blend with biodegradable cellulose acetate in presence of N-(phenyl amino) maleimides.

    abstract：:Wider plastic applications of poly(vinyl chloride) (PVC) has raised serious problem to the environment. Since (PVC) waste products resist biodegradation and persist in the environment for longer time. The object of this study is to blend (PVC) with biodegradable cellulose acetate to thermally support the polymer durin...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.06.033

    authors： Abdel-Naby AS,Al-Ghamdi AA

    更新日期：2014-09-01 00:00:00

  • Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms.

    abstract：:Water-soluble polysaccharides were extracted from the caps and stipes of thirteen boletus mushrooms representing five different species collected in Southwest China. Investigations of their structures and antioxidant activities allowed an evaluation of structure-function relationships. The polysaccharides were compose...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.02.084

    authors： Zhang L,Hu Y,Duan X,Tang T,Shen Y,Hu B,Liu A,Chen H,Li C,Liu Y

    更新日期：2018-07-01 00:00:00

  • Chitosan and gelatin materials incorporated with phenolic extracts of grape seed and jabuticaba peel: Rheological, physicochemical, antioxidant, antimicrobial and barrier properties.

    abstract：:In this study, chitosan and gelatin materials incorporated with grape seed (Vitis vinifera L.) (VSE) and jabuticaba peel (Plinia cauliflora) (PPE) extracts were developed with potential application as food coatings. It was evaluated how the concentration of the extracts and their addition order in the polymeric matrix...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.05.240

    authors： Rodrigues MÁV,Bertolo MRV,Marangon CA,Martins VDCA,Plepis AMG

    更新日期：2020-10-01 00:00:00

  • The effects of daunomycin antibiotic on histone H(1): thermal denaturation and fluorescence spectroscopy studies.

    abstract：:Using thermal denaturation and fluorescence spectroscopy, we have investigated the interaction of antitumor antibiotic, daunomycin, with calf thymus histone H(1) under several ionic strengths. The results show that daunomycin binds to histone H(1) and increases its melting temperature. Increasing ionic strength elevat...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/s0141-8130(00)00146-x

    authors： Zargar SJ,Rabbani A

    更新日期：2000-10-10 00:00:00

  • Antitumor and antiangiogenic activity of Schisandra chinensis polysaccharide in a renal cell carcinoma model.

    abstract：:The aim of this study was to determine the antitumor and antiangiogenic effects of the Schisandra chinensis polysaccharides (SCP) in selected renal cell carcinoma (RCC) cells and evaluate its potential mechanism of action. In vitro, endothelial growth factor (VEGF) secretion by Caki-1 was blockaded in response to SCP ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.02.025

    authors： Qu HM,Liu SJ,Zhang CY

    更新日期：2014-05-01 00:00:00

  • Glycation of hemoglobin leads to the immunogenicity as a result of neo-epitope generation.

    abstract：:Non-enzymatic glycation occurs rapidly which ultimately leads to the formation of advanced glycation endproducts (AGEs). These AGEs have shown to associated with the development of many diseases such as diabetes-mellitus. This study is focused on immunological characterization of glycated-Hb induced by d-ribose. Here,...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.11.063

    authors： Siddiqui Z,Faisal M,Alatar AA,Ahmad S

    更新日期：2019-02-15 00:00:00

  • Sika deer antler protein against acetaminophen-induced nephrotoxicity by activating Nrf2 and inhibition FoxO1 via PI3K/Akt signaling.

    abstract：:Acetaminophen (APAP)-induced acute kidney injury (AKI) has turned into a typical reason for clinic obtained renal disappointment. In any case, the advancement of prophylaxis procedures and endorsed treatments for APAP-AKI is restricted. Sika deer antler protein (SDAPR) has a protective effect on drug-induced AKI. The ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.08.164

    authors： Ruan H,Luo J,Wang L,Wang J,Wang Z,Zhang J

    更新日期：2019-12-01 00:00:00

  • Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.

    abstract：:The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp. were investigated. The molecular weight was estimated to be 4.67 × 105 Da by HPLC-ELSD analysis. Monosaccharide composition was deter...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.06.117

    authors： Wang W,Zhang F,Li Q,Chen H,Zhang W,Yu P,Zhao T,Mao G,Feng W,Yang L,Wu X

    更新日期：2018-10-15 00:00:00

  • pH dependent green synthesis of gold nanoparticles by completely C6-carboxylated curdlan under high temperature and various pH conditions.

    abstract：:A C6-carboxylated curdlan (C6-Cc) obtained from 4-acetamido-TEMPO-mediated oxidation of curdlan was used both as a reducing and stabilizing agent for green synthesis of pH-responsive AuNPs, which was carried out by controlling the pH of the C6-Cc solution at a high temperature (100°C). C6-Cc presented a semi-flexible ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.08.029

    authors： Qiu WY,Wang K,Wang YY,Ding ZC,Wu LX,Cai WD,Yan JK

    更新日期：2018-01-01 00:00:00

  • Functional characterization of a type 2 metallothionein isoform (OsMTI-2b) from rice.

    abstract：:Metallothioneins (MTs) are a family of Cys-rich, low molecular weight, cytoplasmic metal binding proteins. MTs are present in all eukaryotes as well as some prokaryotes. Plant MTs are divided into four types based on Cys distribution pattern in their amino acid sequences. In the present work, the gene encoding OsMTI-2...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.04.021

    authors： Pirzadeh S,Shahpiri A

    更新日期：2016-07-01 00:00:00

  • Unravelling property of polysaccharides from Sargassum sp. as an anti-wrinkle and skin whitening property.

    abstract：:In recent years, the natural ingredients are in high demand in formulating the cosmetic products. Seaweed polysaccharides signify the attractive natural ingredient with a wide range of functional properties that are expected for cosmeceutical formulations. The present work aimed to study the skin protection ability of...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.08.027

    authors： Jesumani V,Du H,Pei P,Zheng C,Cheong KL,Huang N

    更新日期：2019-11-01 00:00:00

  • Structures, properties and application of alginic acid: A review.

    abstract：:Alginic acid is a natural polysaccharide, which has been widely concerned and applied due to its excellent water solubility, film formation, biodegradability and biocompatibility. This paper briefly describes the source, properties, structure and application of sodium alginate by summarizing and analyzing the current ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2020.06.180

    authors： Guo X,Wang Y,Qin Y,Shen P,Peng Q

    更新日期：2020-11-01 00:00:00

  • Inhibition of methemoglobin formation in aqueous solutions under aerobic conditions by the addition of amino acids.

    abstract：:Hemoglobin (Hb) as an important iron-containing oxygen-transport protein is easily oxidized to the ferric met-form, methemoglobin (metHb), and loses the capacity of binding oxygen during storage. In this study, the experimental data indicate that the presence of Tyr and Glu significantly suppress the metHb formation i...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.12.010

    authors： Wei Y,Li C,Zhang L,Su Z,Xu X

    更新日期：2014-03-01 00:00:00

  • Description of a new surface morphology for chitin extracted from wings of cockroach (Periplaneta americana).

    abstract：:In this study a new morphology of chitin, which could find wide applications in the fields of medicine, pharmacy, agriculture, food and textiles, has been described. The chitin was isolated from the wings of Periplaneta americana employing a conventional method. Considering chitin isolation studies conducted previousl...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.01.015

    authors： Kaya M,Baran T

    更新日期：2015-04-01 00:00:00