Investigation on the interaction of acid phosphatase with putrescine using docking, simulations methods and multispectroscopic techniques.


:The effect of putrescine on the dynamics, conformation, and kinetics of acid phosphatase investigated via different experimental and theoretical methods. The Stern-Volmer constants (Ksv) for the acid phosphatase- putrescine compound was obtained at different temperatures. Therefore, putrescine quenched the intensity of the enzyme via the static method. Gibbs free energy displayed which binding process was spontaneous. MD simulation, docking method, and thermodynamic parameters revealed the van der Waals forces and hydrogen bonding that had the specific interaction in unfolding the compound. After putrescine binding, the Vmax amounts of acid phosphatase without changed, and the value of kcat/Km increased. The Tm the acid phosphatase-putrescine compound was decreased. Presumably because of more surface hydrophilicity and further H-bond formation underlying the putrescine modification. As confirmed by fluorescence spectra and UV-Visible spectroscopy. Circular dichroism (CD) and UV absorption investigations also demonstrated which binding of putrescine to acid phosphatase led to microenvironmental variations around the protein. Therefore, putrescine cause changes in its structure and function. The results, therefore, showed the effect of putrescine was because of its kosmotropic specifications. Molecular dynamics simulation and Molecular docking results further confirmed the results obtained by CD and spectroscopy experiments.


Int J Biol Macromol


Moradi S,Shareghi B,Saboury AA,Farhadian S




Has Abstract


2020-05-01 00:00:00












  • Preparation and property of a biantenna macromolecule based on polysialic acid.

    abstract::Polysialic acid (PSA), an acidic polysaccharide usually exists as a double-chain structure on cell adhesion molecules in vertebrates. The available PSA produced from Escherichia coli fermentation, however, is monochain PSA. In this work, a biomimetic biantenna type PSA (biPSA) was synthesized in vitro under mild condi...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Lei Y,Wu M,Wang J,Zhang H,Zhan X,Sun Z,Wu J

    更新日期:2020-07-15 00:00:00

  • UV-mediated synthesis of carboxymethyl cellulose/poly-N-isopropylacrylamide composite hydrogels with triple stimuli-responsive swelling performances.

    abstract::Carboxymethyl cellulose (CMC)/pPoly-N-isopropylacrylamide (PNIPAM) hydrogels were prepared using nanoclay as physical cross-linker by UV irradiation. PNIPAM/CMC hydrogels exhibit 3D network structures with high porosity. PNIPAM/CMC hydrogels exhibit not only high water absorption capacity, but also temperature, pH and...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Su C,Liu J,Yang Z,Jiang L,Liu X,Shao W

    更新日期:2020-10-15 00:00:00

  • Synthesis and characterization of stable and biological active chitin-based polyurethane elastomers.

    abstract::In this work, the preparation of novel biocompatible polyurethane (PU) elastomers were carried out using curcumin and 1,4-butanediol (1,4-BDO) via step growth polymerization reaction of hydroxyl terminated polybutadiene (HTPB), toluene diisocyanate (TDI) and chitin to improve the biocompatibility, antibacterial and an...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Amjed N,Bhatti IA,Zia KM,Iqbal J,Jamil Y

    更新日期:2020-07-01 00:00:00

  • Orabase formulation with cashew gum polysaccharide decreases inflammatory and bone loss hallmarks in experimental periodontitis.

    abstract::This study aimed to investigate the chemical characteristics and the effects of an orabase gel with Cashew Gum Polysaccharide (CG-P) from Anacardium occidentale L. on alveolar bone loss and relative mRNA expression of TNF-α, IL-1β, RANK, RANKL, and OPG in the periodontal tissue of Wistar rats (Rattus norvegicus) subje...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Souza Filho MD,Medeiros JVR,Vasconcelos DFP,Silva DA,Leódido ACM,Fernandes HF,Silva FRP,França LFC,Lenardo D,Pinto GR

    更新日期:2018-02-01 00:00:00

  • Thermal degradation behaviour and crystallization kinetics of poly (lactic acid) and cellulose nanocrystals (CNC) based microcellular composite foams.

    abstract::The current investigation addresses the thermal degradation and non-isothermal crystallization behaviour of the fabricated poly (lactic acid) foam (nPLA) and poly (lactic acid) (PLA)/cellulose nanocrystal (CNC) based foams at three different loadings of CNC (i.e. 1%, 2% and 3%) as PLA/CNC 1, PLA/CNC 2 and PLA/CNC 3 ha...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Borkotoky SS,Chakraborty G,Katiyar V

    更新日期:2018-10-15 00:00:00

  • How to calculate starch lamellar features with improved accuracy by small angle X-ray scattering.

    abstract::Starch lamellar structures unraveled by small angle X-ray scattering (SAXS) have been critically correlated with its functionalities. However, the lamellar features calculated by the classical linear correlation function based on SAXS data are not well convincingly analyzed, because the thickness of amorphous lamellae...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Lin L,Chi C,Wu C

    更新日期:2019-12-01 00:00:00

  • Evaluating the glycophenotype on breast cancer tissues with quantum dots-Cramoll lectin conjugates.

    abstract::During carcinogenesis, changes in the glycosylation can modulate many biological processes. Thus, the interest in exploring and understanding the roles of carbohydrates as cancer biomarkers has been increasing. Lectins have been applied as useful tools in glycobiology, especially when associated with fluorescent repor...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Carvalho MET,Oliveira WF,Cunha CRA,Coelho LCBB,Silva MV,Carvalho Junior LB,Santos BS,Cabral Filho PE,Fontes A,Correia MTS

    更新日期:2019-10-01 00:00:00

  • Barley starch: Structure, properties and in vitro digestibility - A review.

    abstract::Abundance, cheapness, biodegradability and non-toxic behaviour of starches attract many food and non-food industries. Starch provides viscosity, adhesion, texture and homogeneity to various food products. This review article deals with the chemical composition, structure, granular morphology, pasting, thermal and in v...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审


    authors: Punia S

    更新日期:2020-07-15 00:00:00

  • Mechanism of co-aggregation in a protein mixture with small additives.

    abstract::Co-aggregation plays an important role in processing protein-rich food materials under heterogeneous conditions. The main cause of co-aggregation is an electrostatic attraction between oppositely charged molecules. This study investigated thermal aggregation of β-lactoglobulin (BLG) (pI=5.1) and lysozyme (LYZ) (pI=10....

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Oki S,Iwashita K,Kimura M,Kano H,Shiraki K

    更新日期:2018-02-01 00:00:00

  • Characterization and optimization of schizophyllan production from date syrup.

    abstract::This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production. Initially, one factor-at-a-time method was used to find the best carbon and nitrogen sources for schizophyllan production. Subsequently, resp...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Jamshidian H,Shojaosadati SA,Vilaplana F,Mousavi SM,Soudi MR

    更新日期:2016-11-01 00:00:00

  • Physicochemical properties and biological activities of novel blend films using oxidized pectin/chitosan.

    abstract::Pectin has been widely used in a variety of biomedical applications. In this study, it was modified with sodium periodate as an oxidant and characterized by physicochemical methods Periodate oxidation increased the contents of dialdehyde units and carboxyl groups in pectin, and a decrease in pectin viscosity was measu...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Chetouani A,Follain N,Marais S,Rihouey C,Elkolli M,Bounekhel M,Benachour D,Le Cerf D

    更新日期:2017-04-01 00:00:00

  • Extraction of polysaccharides from maca: Characterization and immunoregulatory effects on CD4+ T cells.

    abstract::The immunomodulatory effects of maca polysaccharides (MCPs) on macrophages have been demonstrated in many studies. However, the effects of MCPs on CD4+ T cells have not been studied. Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa)...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Chang Y,Lu W,Chu Y,Yan J,Wang S,Xu H,Ma H,Ma J

    更新日期:2020-07-01 00:00:00

  • Optimization, characterization and evaluation of chitosan-tailored cubic nanoparticles of clotrimazole.

    abstract::The present study deals with improvement of the mucoadhesive properties of monoolein based cubic nanoparticles by incorporating chitosan. Chitosan-tailored cubic nanoparticles were prepared by thin film hydration followed by ultrasonication employing clotrimazole as model drug. The effect of Pluronic F127 fraction and...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Verma P,Ahuja M

    更新日期:2015-02-01 00:00:00

  • Identification and characterization of Pseudomonas aeruginosa derived bacteriocin for industrial applications.

    abstract::Drug resistance has become a major threat due to the frequent use of commercial antibiotics and there is an urgent need to combat this problem. Having this in mind, the present research was aimed at developing a novel P. aeruginosa puBac bacteriocin molecule. The bacteriocin was purified by ammonium sulfate precipitat...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Lakshmanan R,Kalaimurugan D,Sivasankar P,Arokiyaraj S,Venkatesan S

    更新日期:2020-12-15 00:00:00

  • Thermal, mechanical and biodegradation studies of biofiller based poly-3-hydroxybutyrate biocomposites.

    abstract::Biodegradable poly-3-hydroxybutyrate [P(3HB)] and natural fillers - clay, peat, and birch wood flour - were used to prepare powdered composites to form pellets and granules. Pellets were produced by cold pressing of polymer and filler powder whereas granules were produced from the powders wetted with ethanol. Characte...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Thomas S,Shumilova AA,Kiselev EG,Baranovsky SV,Vasiliev AD,Nemtsev IV,Kuzmin AP,Sukovatyi AG,Avinash RP,Volova TG

    更新日期:2020-07-15 00:00:00

  • Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.].

    abstract::Black soybeans are commonly consumed as health foods and used in traditional Chinese medicine, but they are rarely cultivated as edible sprouts. During germination, the composition of seeds undergoes distinct changes that cause variations in bioactivities. In this study, the water-soluble black soybean polysaccharide ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Lee AL,Yu YP,Hsieh JF,Kuo MI,Ma YS,Lu CP

    更新日期:2018-07-01 00:00:00

  • Development of biopolymers based interpenetrating polymeric network of capecitabine: A drug delivery vehicle to extend the release of the model drug.

    abstract::The research aims the development and optimization of capecitabine loaded interpenetrating polymeric network by ionotropic gelation method using polymers locust bean gum and sodium alginate by QbD approach. FMEA was performed to recognize the risks influencing CQAs. BBD was applied to study the effect of factors (poly...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Upadhyay M,Adena SKR,Vardhan H,Yadav SK,Mishra B

    更新日期:2018-08-01 00:00:00

  • Designing a direct ELISA kit for the detection of Staphylococcus aureus enterotoxin A in raw milk samples.

    abstract::Under favorable growth conditions, Staphylococcus aureus infects and causes illnesses in humans and animals. Staphylococcal enterotoxins are considered the most important factor for food poisoning, with these toxins usually found in milk and its byproducts. This study investigated the presence of a classical enterotox...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Nouri A,Ahari H,Shahbazzadeh D

    更新日期:2018-02-01 00:00:00

  • New composites based on starch/Ecoflex®/biomass wastes: Mechanical, thermal, morphological and antimicrobial properties.

    abstract::Different biomass wastes were successfully blended with starch and Ecoflex® viz. poly(butylene adipate-co-terephthalate), without glycerol addition, to obtain biocomposite materials. The mechanical properties, as well as thermal and surface properties, of the developed composites were evaluated. It was found that the ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Spiridon I,Anghel NC,Darie-Nita RN,Iwańczuk A,Ursu RG,Spiridon IA

    更新日期:2020-08-01 00:00:00

  • Implications for glycosylated compounds and their anti-cancer effects.

    abstract::Glycosylated compounds are major secondary metabolites of plants, which have various therapeutic effects on human diseases, by acting as anti-cancer, antioxidant, and anti-inflammatory agents. Glycosylation increases stability, bioactivity, and solubility of compounds and improves their pharmacological properties. Two...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审


    authors: Pourakbari R,Taher SM,Mosayyebi B,Ayoubi-Joshaghani MH,Ahmadi H,Aghebati-Maleki L

    更新日期:2020-11-15 00:00:00

  • Identification and characterization of a polysaccharide from the roots of Morinda officinalis, as an inducer of bone formation by up-regulation of target gene expression.

    abstract::Morinda officinalis is an important traditional tonic herbal medicine. In the present study, we found that crude polysaccharides extracted from M. officinalis, named MO90, could significantly increase the bone mineral density (BMD) of the whole femur, distal femur, and proximal femur in ovariectomized (OVX) rats. In a...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Yan C,Huang D,Shen X,Qin N,Jiang K,Zhang D,Zhang Q

    更新日期:2019-07-15 00:00:00

  • Release of DNA from surfactant complexes induced by 2-hydroxypropyl-beta-cyclodextrin.

    abstract::Decompaction of DNA-CTA self-assembled complexes by 2-hydroxypropyl-beta-cyclodextrin (2-HP-beta-CD) was studied and the results were compared with beta-CD. Different degrees of 2-HP substitution (0.6, 0.8 and 1.0, respectively) were used and the decompaction was successful with all degrees of substitution. Fluorescen...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Carlstedt J,González-Pérez A,Alatorre-Meda M,Dias RS,Lindman B

    更新日期:2010-03-01 00:00:00

  • A non-naturally-occurring P(3HB-co-3HAMCL) is produced by recombinant Pseudomonas sp. from an unrelated carbon source.

    abstract::Pseudomonas sp. PHA- was used as host for PHA biosynthesis genes from Aeromonas sp. to produce 3HB-co-3HAMCL from glucose with no supply of co-substrates. A non-naturally-occurring PHA composed mainly of 3HB, 3HHx and 3HD (3HO, 3HDdΔ5 and 3HDd monomers were detected in smaller amounts) was obtained. The polymer was ex...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Cespedes LG,Nahat RATPS,Mendonça TT,Tavares RR,Oliveira-Filho ER,Silva LF,Taciro MK,Sánchez RJ,Gomez JGC

    更新日期:2018-07-15 00:00:00

  • Effect of the addition of modified starch on gelatinization and gelation properties of rice flour.

    abstract::The objective of this work is to research impacts of oxidized (OCS), acetylated (ACS) and cross-linked (CLCS) corn starch on gelatinization properties of rice flour and their gel structure during storage at 4 °C for 1 and 7 days. OCS led to significant increase of pasting temperature and solubility in blend flour but ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Hu WX,Chen J,Zhao JW,Chen L,Wang YH

    更新日期:2020-06-15 00:00:00

  • Starch-based polyurethane/CuO nanocomposite foam: Antibacterial effects for infection control.

    abstract::In the present study, a new method for the synthesis of the open cell flexible polyurethane foams (PUFs) was developed by using starch powder and the modification of closed cell foam formulation. Starch is the second largest polymeric carbohydrate as a macromolecule on this planet with a large number of glucose units....

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Ashjari HR,Dorraji MSS,Fakhrzadeh V,Eslami H,Rasoulifard MH,Rastgouy-Houjaghan M,Gholizadeh P,Kafil HS

    更新日期:2018-05-01 00:00:00

  • Oral delivery of nanoparticles containing anticancer SN38 and hSET1 antisense for dual therapy of colon cancer.

    abstract::An oral delivery system intended for treatment of colon cancer in HT29 cancerous cells was investigated by encapsulating hSET1 antisense and SN38 anticancer in nanoparticles based on cysteine trimethyl chitosan (cysTMC) and carboxymethyl dextran (CMD). Studies have shown hSET1 as the main type of histone methyltransfe...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Dinarvand M,Kiani M,Mirzazadeh F,Esmaeili A,Mirzaie Z,Soleimani M,Dinarvand R,Atyabi F

    更新日期:2015-01-01 00:00:00

  • The effect of deep eutectic solvents on catalytic function and structure of bovine liver catalase.

    abstract::Aqueous solutions of reline and glyceline, the most common deep eutectic solvents, were used as a medium for Catalase reaction. By some spectroscopic methods such as UV-vis, fluorescence and circular dichroism (CD) function and structure of Catalase were investigated in aqueous solutions of reline and glyceline. These...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Harifi-Mood AR,Ghobadi R,Divsalar A

    更新日期:2017-02-01 00:00:00

  • Microwave synthesis, characterization, and zinc uptake studies of starch-graft-poly(ethylacrylate).

    abstract::Microwave synthesis of starch-graft-poly(ethylacrylate) was optimized to obtain efficient Zn(II) adsorbent that has been characterized using IR, XRD and SEM analyses. Using the sample, adsorption of Zn(II) was studied as a function of pH, sorbent dose, initial Zn(II) concentration, % grafting, temperature and ionic st...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Singh V,Maurya S

    更新日期:2010-10-01 00:00:00

  • A new water-soluble polysaccharide from Echinops pungens Trautv roots. Part I. Isolation, purification, characterization and antioxidant activity.

    abstract::EPPS, a new heteropolysaccharide from Echinops pungens roots, was extracted by warm water (70 °C) and fortuitously purified using column chromatographies on DEAE-cellulose A52 and Sephadex G-100. Total carbohydrate content, specific optical rotation and molecular mass of EPPS were 98.3%, -88.2 and 65.7 kDa, respective...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Hoseiniyan Benvidi SM,Jahanbin K

    更新日期:2020-10-15 00:00:00

  • Chitosan cross-linked docetaxel loaded EGF receptor targeted nanoparticles for lung cancer cells.

    abstract::Lung cancer, associated with the up-regulated epidermal growth factor receptor (EGFR) led to the development of EGFR targeted anticancer therapeutics. The biopolymeric nanoparticles form an outstanding system for the targeted delivery of therapeutic agents. The present work evaluated the in vitro effects of chitosan c...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Maya S,Sarmento B,Lakshmanan VK,Menon D,Seabra V,Jayakumar R

    更新日期:2014-08-01 00:00:00