Abstract：
:hRSV is the major causative agent of acute respiratory infections. Among its eleven proteins, M2-1 is a transcription antiterminator, making it an interesting target for antivirals. Quercetin is a flavonol which inhibits some virus infectivity and replication. In the present work, the M2-1 gene was cloned, expressed and the protein was purified. Thermal stability and secondary structure were analyzed by circular dichroism and the interaction with Quercetin was evaluated by fluorescence spectroscopy. Molecular docking experiments were performed to understand this mechanism of interaction. The purified protein is mainly composed of α-helix, with a melting temperature of 328.6K (≈55°C). M2-1 titration with Quercetin showed it interacts with two sites, one with a strong constant association K1 (site 1≈1.5×106M-1) by electrostatic interactions, and another with a weak constant association K2 (site 2≈1.1×105M-1) by a hydrophobic interaction. Ligand's docking shows it interacts with the N-terminus face in a more polar pocket and, between the domains of oligomerization and RNA and P protein interaction, in a more hydrophobic pocket, as predicted by experimental data. Therefore, we postulated this ligand could be interacting with important domains of the protein, avoiding viral replication and budding.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Teixeira TS,Caruso ÍP,Lopes BR,Regasini LO,Toledo KA,Fossey MA,Souza FPdoi
10.1016/j.ijbiomac.2016.11.033subject
Has Abstractpub_date
2017-02-01 00:00:00pages
63-71eissn
0141-8130issn
1879-0003pii
S0141-8130(16)32356-Xjournal_volume
95pub_type
杂志文章abstract：:A high molecular weight levan was produced by a novel levansucrase and some properties of this polymer were investigated. The levan exhibited a poroid microstructure as well as series of individual ellipsoidal or spheroidal particles. The weight-average molecular weight (M¯w) of the levan was determined to be 1.41×108...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.11.056
更新日期：2018-04-01 00:00:00
abstract：:Octenyl succinic anhydride modified inulin (In-OSA) was synthesized via chemical modification of inulin with octenyl succinic anhydride (OSA). The esterification of inulin with OSA was confirmed by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and degree of substitution (DS) calc...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.03.067
更新日期：2015-01-01 00:00:00
abstract：:Due to the problems raised by the use of animal or microbial recombinant proteases, the use of proteases from vegetable origin is becoming increasingly popular.. Among them, sulfidryl proteases have a special interest. Ficin is an outstanding example of this kind of proteases. This paper aims to be to make a comprehen...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2020.06.144
更新日期：2020-11-01 00:00:00
abstract：:Heparan sulfate (HS) is a linear anionic polysaccharide with repeating sulfated disaccharide units, which has been proven with various regulatory osteogenesis effects through multi-pathway signaling, but its impacts on receptor-activator of nuclear factor kappa beta ligand/receptor-activator of nuclear factor kappa be...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.07.308
更新日期：2020-10-15 00:00:00
abstract：:This study aimed to investigate the chemical characteristics and the effects of an orabase gel with Cashew Gum Polysaccharide (CG-P) from Anacardium occidentale L. on alveolar bone loss and relative mRNA expression of TNF-α, IL-1β, RANK, RANKL, and OPG in the periodontal tissue of Wistar rats (Rattus norvegicus) subje...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.09.087
更新日期：2018-02-01 00:00:00
abstract：:In this study, gellan polymer was conferred amphiphilic character by conjugating alkyl carbon chain (C16) to its backbone via etherification reaction. The amphiphilic copolymer self-assembled into water and formed spherical micellar structures with a mean diameter of 832 nm. Copolymer micellization caused a considerab...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.09.064
更新日期：2015-01-01 00:00:00
abstract：:Levansucrase (LS) from Gram-positive bacteria generally produces a large quantity of levan polymer, a polyfructose with glucose at the end (GFn) but a small quantity of levan-type fructooligosaccharides (LFOs). The properties of levan and LFOs depend on their chain lengths, thereby determining their potential applicat...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.08.151
更新日期：2019-11-01 00:00:00
abstract：:4-O-Methylglucuronoxylans (MGX) were isolated from chestnut wood sawdust using two different procedures: chlorite delignification followed by the classical alkaline extraction step, and an unusual green chemistry process of delignification using phthalocyanine/H2O2 followed by a simple extraction with hot water. Antio...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.07.005
更新日期：2014-09-01 00:00:00
abstract：:Laccase is one of a few enzymes that can directly reduce oxygen into water under ambient conditions, while oxidizing a variety of aromatic compounds. Its conjugation with chitosan generates a pH-sensitive functional biomaterial that changes its solubility in response to pH variation. The molecular conjugation between ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2005.01.003
更新日期：2005-03-01 00:00:00
abstract：:The ever-increasing applications of enzymes are limited by the relatively poor performance in harsh processing conditions. As a result, there are constant innovations in immobilization protocols for improving biocatalyst activity and stability. Bacterial spores are cheap to generate and highly resistant to environment...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2020.10.171
更新日期：2021-01-01 00:00:00
abstract：:The aim of this work was to evaluate the possibility of control of wilt disease caused by Fusarium andiyazi through chitosan (CS) and chitosan nanoparticles (CNPs). In the present study, the expression pattern of pathogenesis-related (PR) proteins genes such as PR-1, PR-2 (β-1,3-glucanase), PR-8 (chitinase), and PR-10...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.12.167
更新日期：2019-03-15 00:00:00
abstract：:The interaction of novel oligo(L-lysine)-shelled dendrimers (G3-PLL) with DNA was studied by means of circular dichroism spectroscopy, dynamic light scattering, and melting behavior of double-stranded DNA. G3-PLLs having various oligo(L-lysine) (PLL) segment (n = 5-40) were successfully synthesized by graft-polymeriza...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2006.01.017
更新日期：2006-03-30 00:00:00
abstract：:Whey protein isolate (WPI)-based composite films with varying proportions of oat husk nanocellulose (ONC) obtained from acid sulfuric hydrolysis were prepared using a solution casting method. The obtained material after each step of the isolating cellulose, morphological and crystallinity of the ONC were studied by Fo...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.06.077
更新日期：2016-10-01 00:00:00
abstract：:Interferon-inducible transmembrane protein 3 (IFITM3) inhibits the replication of multiple pathogenic viruses by blocking their entry. In this study, we constructed a shuttle plasmid, harboring human IFITM3. Thereafter, recombinant adenovirus rAd5-IFITM3 was obtained by co-transfection of the linearized viral backbone...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.03.161
更新日期：2019-06-15 00:00:00
abstract：:Brasenia schreberi (B. schreberi) is a rare and precious vegetable, which coat with a gelatinous mucilage. This study is aiming to investigate the relationship between structural characteristics and hypoglycemic activities of polysaccharides with different molecular weight (Mw) range. Three polysaccharides fractions, ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.05.129
更新日期：2019-08-15 00:00:00
abstract：:The effects of the incorporation of PLA-β-cyclodextrin-inclusion complex (IC) and β-cyclodextrin (β-CD) on biopolyester PLA films were investigated. Thermal stability, surface morphology, barrier, and mechanical properties of the films were measured at varying IC (1, 3, 5, and 7%) and β-CD (1 and 5%) concentrations. T...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.08.036
更新日期：2015-11-01 00:00:00
abstract：:Crosslinked chitosan beads were grafted with N-vinyl-2-pyrrolidone (NVP) using ammonium persulfate (APS) as free radical initiator. Important variables on graft copolymerization such as temperature, reaction time, concentration of initiator and concentration of monomer were optimized. The results revealed optimum cond...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.09.061
更新日期：2018-02-01 00:00:00
abstract：:In present study, HEP was successfully encapsulated into the Poly (lactic-coglycolicacid) (PLGA) to constitute the HEP-PLGA. The effects of three independent factors (the proper range of ratio of organic phase (o) to internal water phase (w1) (X1), ratio of external water phase (w2) to the primary emulsion (PE) (X2), ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.06.169
更新日期：2018-10-15 00:00:00
abstract：:In this work, amino-functionalized Santa Barbara Amorphous-15/calcium alginate (ASBA-15@CA) microspheres were fabricated and characterized by FTIR, SEM, XRD, TGA, and XPS analyses. The Pb(II) adsorption properties of the ASBA-15@CA microspheres were studied in terms of the effects of the adsorption time, adsorbent dos...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.12.112
更新日期：2019-03-15 00:00:00
abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ubiquitous enzyme involved in glycolysis. It is also referred to as a moonlighting protein as it has many diverse functions like regulation of apoptosis, iron homeostasis, cell-matrix interactions, adherence to human colon etc. apart from its principal role in glyc...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.04.041
更新日期：2016-10-01 00:00:00
abstract：:Aliquat-336 (an ionic liquid) impregnated sulphate-crosslinked chitosan (SCC) was prepared for escalating the adsorption of hexavalent chromium through concurrent interaction. The compound obtained was intensively characterized using Fourier transform infra red (FT-IR), X-ray diffraction (XRD), Scanning electron micro...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.07.035
更新日期：2015-09-01 00:00:00
abstract：:Chitooligosaccharides (COS) are hydrolytic products of chitosan that are essential in functional food, medicine, and other fields due to their biological activities. Commercial COS are often prepared by the hydrolysis of chitosan by chitosanase. In this study, a glycoside hydrolase family 46 cluster B chitosanase from...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.02.001
更新日期：2020-04-15 00:00:00
abstract：:Chitooligosaccharides (COS) display a variety of important biological activities, including antimicrobial, antitumor, anti-inflammatory, and immunoenhancing. In the present study, a COS sample (degree of polymerization 4-11, analyzed by FTIR and MALDI-TOF-MS) prepared by hydrolysis with a recombinant chitosanase was o...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.09.038
更新日期：2013-11-01 00:00:00
abstract：:In this work three dimensional biodegradable nanofiberous scaffolds containing poly(ε-caprolactone) (PCL), poly(vinyl alcohol) (PVA) and gum tragacanth (GT) were successfully fabricated through two nozzles electrospinning process. For this purpose, PVA/GT blend (Blend: B) solution (60:40wt%) was injected from one syri...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.10.042
更新日期：2017-01-01 00:00:00
abstract：:The influence of metal cations on G-quadruplex structure and peroxidase-mimicking DNAzyme activity was investigated. Experiments revealed a significant role of magnesium ion, which in the presence of potassium cation influenced DNAzyme activity. This ability has been associated with alteration of G-quadruplex topology...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.01.020
更新日期：2016-04-01 00:00:00
abstract：:A polysaccharide YCP was prepared from a marine filamentous fungus Keissleriella sp. YS4108, which exhibited as a molecular weight (Mw) of 2.4x10(3) kDa and its three sulfated derivatives (YCP-SL, YCP-SM and YCP-SH) were synthesized, the degree of substitution (DS) of which were determined to be 0.13, 0.99 and 1.3, wi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2005.06.003
更新日期：2005-09-15 00:00:00
abstract：:This study describes the use of lignin as natural adhesive for the production high density fiberboards (HDF) made from wheat straw. In the present work, this agricultural residue was used to produce thermomechanical pulp and the used lignin was obtained from the spent liquors generated in the same process. A hot press...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.11.005
更新日期：2018-03-01 00:00:00
abstract：:In this study, the cells-free and cells-loaded chitosan hydrochloride-alginate (CHC-Alg) microcapsules were firstly fabricated with polyelectrolyte complexes via an orifice-polymerization method. Scanning electron microscope images showed that the CHC-Alg microcapsules had a typical shell-core structure and the model ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.09.034
更新日期：2016-12-01 00:00:00
abstract：:The dawn of molecular biology and recombinant DNA technology arose from our ability to manipulate DNA, including the process of collapse of long extended DNA molecules into nanoparticles of approximately 100 nm diameter. This condensation process is important for the packaging of DNA in the cell and for transporting D...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2017.12.076
更新日期：2018-04-01 00:00:00
abstract：:A new green hydrogel has been synthesized by crosslinking lignosulfonate (L), sodium alginate (SA) and konjaku flour (KJ). We have optimized the ratio of the three synthesized polymers using an orthogonal design of experiments and characterized this hydrogel using SEM, BET and FTIR spectra. We have added the hydrogel ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.12.082
更新日期：2020-02-01 00:00:00
