Abstract:
:Given the excellent characteristics of alginate, it is an industrially important polysaccharide. Mannuronan C5-epimerase (MC5E) is an alginate-modifying enzyme that catalyzes the conversion of β-D-mannuronate (M) to its C5 epimer α-L-guluronate (G) in alginate. Both the biological activities and physical properties of alginate are determined by M/G ratios and distribution patterns. Therefore, MC5E is regarded as a biotechnological tool for modifying and processing alginate. Various MC5Es derived from brown algae, Pseudomonas and Azotobacter have been isolated and characterized. With the rapid development of structural biology, the crystal structures and catalytic mechanisms of several MC5Es have been elucidated. It is necessary to comprehensively understand the research status of this alginate-modifying enzyme. In this review, the properties and potential applications of MC5Es isolated from different kinds of organisms are summarized and reviewed. Moreover, future research directions of MC5Es as well as strategies to enhance their properties are elucidated, highlighted, and prospected.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Ci F,Jiang H,Zhang Z,Mao Xdoi
10.1016/j.ijbiomac.2020.11.123subject
Has Abstractpub_date
2021-01-31 00:00:00pages
663-675eissn
0141-8130issn
1879-0003pii
S0141-8130(20)35048-0journal_volume
168pub_type
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