Abstract：
:In the present study, pear polyphenol oxidase (PPO) was purified, immobilized and applied for the synthesis of theaflavin-3,3'-digallate (TF3). Firstly, PPO of pear (Pyrus bretschneideri Rehd cv. Huangguan) was purified 24.36-fold in specific activity with a recovery of 6.77% by sequential use of precipitation with 70% saturated ammonium sulfate and chromatography of DEAE-Sepharose Fast Flow column. The purified pear PPO, found to be a dimer of identical subunits of molecular mass 35kDa, was then successfully immobilized onto Fe3O4/chitosan nanoparticles via glutaraldehyde coupling reaction. Finally, TF3 was effectively synthesized by applying the immobilized PPO as biocatalyst with epicatechin gallate (ECG) and epigallocatechin-3-O-gallate (EGCG) as substrates, and the maximum yield of TF3 was 42.23% based on the amount of ECG added. In addition, the immobilized enzyme still remained 85% of its initial ability after successive usage of 8 cycles and could be stored for 30days with less loss of activity. All the results suggest that the developed procedure is quite useful and has great potential for synthesis of TF3 and other theaflavins.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Lei S,Xie M,Hu B,Zhou L,Sun Y,Saeeduddin M,Zhang H,Zeng Xdoi
10.1016/j.ijbiomac.2016.10.072subject
Has Abstractpub_date
2017-01-01 00:00:00pages
709-718issue
Pt Aeissn
0141-8130issn
1879-0003pii
S0141-8130(16)31077-7journal_volume
94pub_type
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