Abstract：
:The current study establishes the necessity of Glu-381, Glu-384, and Glu-385 in the βDELSEED-motif of Escherichia coli ATP synthase for peptide binding and inhibition. Inhibitory profiles of wild type and mutant E. coli membrane bound F1Fo ATP synthase were studied in the presence and absence of C-terminal NH2 bound melittin. Melittin-NH2 caused almost complete inhibition of wild type ATPase, while partial inhibition was observed for mutants where Glu was replaced with Ala, Arg, or Gln. Additionally, melittin-NH2 caused insignificant inhibition of a triple mutant where all three Glu residues were replaced with Ala residues, changing βDELSEED-motif to βDALSAAD-motif. Little or partial loss of oxidative phosphorylation in mutant strains corroborates their distinct location away from the catalytic site of ATP synthase. Moreover, abrogation of wild type E. coli cell growth and normal growth of mutant stains in the presence of melittin-NH2 further validated the necessity of Glu residues in the βDELSEED-motif for peptide binding. Overall, while loss of one Glu residue at a time may allow partial peptide binding, loss of three Glu residues together-βE381, βE384, and βE385-is detrimental for peptide binding and inhibition of ATP synthase.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Azim S,Ahmad Zdoi
10.1016/j.ijbiomac.2018.05.118subject
Has Abstractpub_date
2018-09-01 00:00:00pages
977-982eissn
0141-8130issn
1879-0003pii
S0141-8130(18)31703-3journal_volume
116pub_type
杂志文章abstract：:In this study, a new matrix for immobilization of acetylcholinesterase was investigated by using alginate and kappa-carrageenan. The effects of pH, temperature, storage and thermal stability on the free and immobilized acetylcholinesterase activity were examined. Maximum reaction rate (V(max)) and Michaelis-Menten con...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2005.10.003
更新日期：2005-11-15 00:00:00
abstract：:A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent. Chitosan (CS) was coupled with a hydrophobic nonanal (N) to form nonanyl chitosan (NCS) schiff base, and followed by grafting with butyl acrylate monomers (ButA). The grafted copolym...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.08.169
更新日期：2019-11-01 00:00:00
abstract：:The purpose of this work was to develop and characterize chitosan-alginate beads for the extended delivery of cefpodoxime proxetil (CFP), to understand the impact of formulation and process parameters on the critical quality attributes (CQAs) using a quality-by-design approach. For this, a study was performed with var...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.05.066
更新日期：2014-08-01 00:00:00
abstract：:Barley starch citrate (BSC), a type IV resistant starch (RS) is associated with numerous health benefits when incorporated in bakery products. Physicochemical, pasting and rheological properties of the wheat starch (WS) and wheat starch-barley starch citrate (WS-BSC) blends and textural and sensorial attributes of whe...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.03.009
更新日期：2019-06-01 00:00:00
abstract：:Colorectal cancer has an overexpression of galectin-3 that is related to cancer progression. A decreased risk of colon cancer can be related to consumption of dietary fibers, but the entire mechanism by which this protection occurs remains unclear. Pectin is a type of dietary fiber that possesses β-galactosides and ca...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.12.191
更新日期：2019-04-01 00:00:00
abstract：:An antimicrobial packaging material was developed by uniformly embedding 1, 3 and 5% chitosan (w/w) in low density polyethylene matrix using maleic anhydride grafted LDPE as a compatible agent. The materials were mixed by compounding and blown into monolayer films via blown film extrusion. The developed films showed g...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.06.016
更新日期：2015-08-01 00:00:00
abstract：:Concanavalin A (Con A) binding fraction of human seminal plasma is vital as it shows decapacitating activity and contains proteins which have critical roles in fertility related processes. Con A binding proteins were isolated by lectin affinity chromatography. These proteins form high molecular weight aggregates at ne...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.11.019
更新日期：2013-02-01 00:00:00
abstract：:Thyroid hormone receptor interactor 13 (TRIP13) has been reported to be overexpressed in serval types of human cancers, and regulate tumor cell proliferation, migration and invasion. However, the role of TRIP13 in prostate cancer was still unclear. In our study, the correlation between TRIP13 expression and clinical p...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.09.168
更新日期：2019-01-01 00:00:00
abstract：:In this paper, the electronspun Polylactic acid (PLA)/TiO2 nanofluids (nfs) fibrous membrane with good toughness, hydrophilicity and antibacterial activities are fabricated by taking full advantages of solvent-free TiO2 nfs with amphiphilicity and ionic conductivity. The resulting PLA/TiO2 nfs fibrous membrane exhibit...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.06.027
更新日期：2020-10-15 00:00:00
abstract：:In the present study, Penicillium proteolyticum lectin was purified by DEAE-Sepharose chromatography followed by Sephadex G-100 gel filtration chromatography. A sequence of ion-exchange and gel filtration chromatography resulted in 52.30 fold purified lectin with a high yield of 84.21%. The purified P. proteolyticum l...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.01.103
更新日期：2019-05-01 00:00:00
abstract：:Laccase is one of a few enzymes that can directly reduce oxygen into water under ambient conditions, while oxidizing a variety of aromatic compounds. Its conjugation with chitosan generates a pH-sensitive functional biomaterial that changes its solubility in response to pH variation. The molecular conjugation between ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2005.01.003
更新日期：2005-03-01 00:00:00
abstract：:Keeping in view the importance of polysaccharide gum in the pharmaceutical formulations, in the present work, exploration of the potential of the moringa gum in hydrogel formation for drug delivery applications has been carried out. The gum based hydrogels were prepared via radiation induced graft-copolymerization of ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.09.148
更新日期：2018-12-01 00:00:00
abstract：:Amphiphilic chitosans, which may self-assemble in aqueous solution to form nanoaggregates with different conformations depending to the environmental pH, can be used as drug transport and delivery agents, when the target pH differs from the delivery medium pH. In this study, quercetin, a bioactive flavonoid, was encap...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.08.056
更新日期：2018-01-01 00:00:00
abstract：:The current communication reports the inhibitory effect of para-benzoquinone (p-BQ) on the structure and function of bovine liver catalase (BLC), a vital antioxidant enzyme. Both BLC and p-BQ were dissolved in respective buffers and the biophysical interaction was studied at physiological concentrations. For the first...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.11.044
更新日期：2021-01-15 00:00:00
abstract：:Multienzymatic conversion of carbon dioxide (CO2) into chemicals has been extensively studied. However, regeneration and reuse of co-factor are still the main problems for the efficient conversion of CO2. In this study, a nanoscale multienzyme reactor was constructed by encapsulating simultaneously carbonic anhydrase ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.03.177
更新日期：2020-07-15 00:00:00
abstract：:The role of polyglutamine (polyQ) tract on protein stability and disease pathology remains ambiguous. We monitored the unfolding/refolding patterns of huntingtin proteins with varying polyQ lengths. In the presence of urea, minor differences in unfolding and refolding efficiencies were observed. However, in the presen...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.07.022
更新日期：2012-12-01 00:00:00
abstract：:The thermal helix-coil transition in UV irradiated collagen solution, collagen film and pieces of rat tail tendon (RTT) were compared. Their thermal stability's were determined by differential scanning calorimeter (DSC) and by viscometric measurements. The denaturation temperatures of collagen solution, film and piece...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(99)00047-1
更新日期：1999-05-01 00:00:00
abstract：:Silica nanoparticles (SiO2 NPs) have been widely used in the medical and food sciences. However, their toxic effects against bio-macromolecules and cells are not well understood. The present study was aimed to investigate the adverse effects of fabricated SiO2 NPs on the human hemoglobin (Hb) by FTIR, CD, fluorescence...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.06.128
更新日期：2018-10-15 00:00:00
abstract：:Brasenia schreberi (B. schreberi) is a rare and precious vegetable, which coat with a gelatinous mucilage. This study is aiming to investigate the relationship between structural characteristics and hypoglycemic activities of polysaccharides with different molecular weight (Mw) range. Three polysaccharides fractions, ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.05.129
更新日期：2019-08-15 00:00:00
abstract：:A fraction of low mobility group (LMG) nonhistone protein designated LMG(160) was isolated from rat liver chromatin by preparative gel electrophoresis and its interaction with DNA was studied using thermal denaturation and DNA-cellulose affinity chromatography techniques. The results showed that LMG(160) with an isoel...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(02)00084-3
更新日期：2003-01-15 00:00:00
abstract：:In this work, amino-functionalized Santa Barbara Amorphous-15/calcium alginate (ASBA-15@CA) microspheres were fabricated and characterized by FTIR, SEM, XRD, TGA, and XPS analyses. The Pb(II) adsorption properties of the ASBA-15@CA microspheres were studied in terms of the effects of the adsorption time, adsorbent dos...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.12.112
更新日期：2019-03-15 00:00:00
abstract：:In this study, the complete mitochondrial DNA (mtDNA) sequence of the crab Parasesarma affine is determined, characterized and compared with other decapod crustaceans. The P. affine mitochondrial genome (mitogenome) is 15,638 bp in size, and contains 13 protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 tra...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.06.056
更新日期：2018-10-15 00:00:00
abstract：:This study reports encapsulation-vitrification of Leydig cells. The Leydig cells were encapsulated in sodium alginate beads of different sizes and cryopreserved by vitrification or slow freezing. Physico-chemical characterization of beads was done by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (X...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.02.233
更新日期：2020-06-15 00:00:00
abstract：:Lung cancer, associated with the up-regulated epidermal growth factor receptor (EGFR) led to the development of EGFR targeted anticancer therapeutics. The biopolymeric nanoparticles form an outstanding system for the targeted delivery of therapeutic agents. The present work evaluated the in vitro effects of chitosan c...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.06.009
更新日期：2014-08-01 00:00:00
abstract：:Mishmi takin (Budorcas taxicolor taxicolor) is an endangered and least studies species. We sequenced and annotated a 16,584 bp long complete mitogenome of Mishmi takin (B. t. taxicolor). It was encoded by 37 genes: 13 Protein-Coding Genes (PCGs), two ribosomal RNA genes, 22 transfer RNA, and non-coding control region....
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.06.201
更新日期：2019-09-15 00:00:00
abstract：:Antioxidant effects of lentinan on LPS induced inflammatory response in intestine of juvenile taimen were evaluated, and its prebiotic-like efficacy on intestinal microbiota was also investigated. The results showed that LPS decreased the activities of antioxidant enzymes and increased the expression levels of inflamm...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.09.121
更新日期：2019-01-01 00:00:00
abstract：:This study aimed to reveal the effect of corn oil on formation of complex between rice starch and fatty acids, including lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0) and stearic acid (C18:0). The RVA, XRD, DSC and CLSM analysis confirmed formation of starch-fatty acid complex but the starch-trigly...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.11.072
更新日期：2020-07-15 00:00:00
abstract：:Cysteine-Rich Secretory Proteins (CRISP) are widespread in snake venoms and known to target ion channels. More recently, CRISPs have been shown to mediate inflammatory responses. Involvement of potential receptor in CRISP-induced inflammatory reactions, however, remains unknown. A CRISP protein named as Nk-CRISP, was ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.05.169
更新日期：2020-10-01 00:00:00
abstract：:Experiments were conducted on microencapsulation of garlic oleoresin by spray drying with garlic oleoresin concentration (10%, 20% and 30%) as core material, maltodextrin concentration (40%, 50% and 60%) as wall material and inlet temperature of drying air (180 °C, 200 °C and 220 °C) as process parameters. The process...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.08.011
更新日期：2015-01-01 00:00:00
abstract：:In this study, the physicochemical and in vitro digestion of millet starch with different moisture content after microwave treatment were investigated. The structure and property changes of native (NA) and microwave treated (MT) millet starches were characterized using Brabender viscometer, Fourier transforms infrared...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.05.046
更新日期：2019-08-01 00:00:00
