Extraction, purification and anti-osteoporotic activity of a polysaccharide from Epimedium brevicornum Maxim. in vitro.

Abstract:

:Osteoporosis is the most widespread metabolic bone disease characterized by decreased bone mass and bone quality, and its diagnosis and treatment remains challenging. To date, medicinal plants have received increasing attention from researchers for researching effective and less toxic therapeutic ingredients, including polysaccharide, to treat osteoporosis. The present study aims to evaluate the osteoprotective effects of a polysaccharide (EBP) from Epimedium brevicornum in glucocorticoid-induced osteoporosis in vitro and investigate the underlying mechanism. EBP (25 and 100 μg/ml) pretreatment could significantly prevent decreased cell proliferation of osteoblasts (OBs) treated only with 100 μM of dexamethasone (Dex) via induction of apoptosis. The osteoblastic differentiation of EBP pretreatment on OBs at early and later phase was further confirmed by the increased alkaline phosphatase (ALP) activity and calcium content, respectively. Meanwhile, the increased expression of cleaved caspase-3 and Bax, as well as a decrease of Bcl-2 and the phosphorylation of PI3K, Akt and mTOR protein in Dex-treated OBs were totally reversed by EBP pretreatment. Moreover, the protein expression of Lrp-5, β-catenin, Runx2 and Osx were significantly up-regulated in the presence of EBP pretreatment. In conclusion, these results demonstrated that EBP pretreatment may be a potential therapeutic agent for patients with glucocorticoid-induced osteoporosis (GIO).

journal_name

Int J Biol Macromol

authors

Zheng H,He B,Wu T,Cai J,Wei J

doi

10.1016/j.ijbiomac.2019.11.145

subject

Has Abstract

pub_date

2020-08-01 00:00:00

pages

1135-1145

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(19)36012-X

journal_volume

156

pub_type

杂志文章
  • Gene expression studies and molecular characterization of a cathepsin L-like from the Asian citrus psyllid Diaphorina citri, vector of Huanglongbing.

    abstract::Huanglongbing (HLB) is a devastating citrus disease associated with Candidatus Liberibacter asiaticus (CLas) and is transmitted by the psyllid Diaphorina citri Kuwayama. Diaphorina citri belongs to Hemiptera order, which has cysteine peptidases as the most abundant proteolytic enzymes present in digestive tract. As cy...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.04.070

    authors: Ferrara TFS,Schneider VK,Lima PS,Bronze FS,Machado MFM,Henrique-Silva F,Soares-Costa A,Carmona AK

    更新日期:2020-04-30 00:00:00

  • Anti-Parkinsonian L-Dopa can also act as anti-systemic amyloidosis-A mechanistic exploration.

    abstract::In spite of the fact that amyloid related neurodegenerative illnesses and non-neuropathic systemic amyloidosis have allured the research endeavors, as no cure has been announced yet apart from symptomatic treatment. Therapeutic agents which can reduce or disaggregate those toxic oligomers and fibrillar species have be...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.03.028

    authors: Nusrat S,Zaidi N,Siddiqi MK,Zaman M,Siddique IA,Ajmal MR,Abdelhameed AS,Khan RH

    更新日期:2017-06-01 00:00:00

  • Montmorillonite/graphene oxide/chitosan composite: Synthesis, characterization and properties.

    abstract::The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method. The formations of films were verified by Fourier transform infrared (FT-IR) ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.05.055

    authors: Yadav M,Ahmad S

    更新日期:2015-08-01 00:00:00

  • Evaluation of sodium alginate for encapsulation-vitrification of testicular Leydig cells.

    abstract::This study reports encapsulation-vitrification of Leydig cells. The Leydig cells were encapsulated in sodium alginate beads of different sizes and cryopreserved by vitrification or slow freezing. Physico-chemical characterization of beads was done by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (X...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.02.233

    authors: Patra T,Gupta MK

    更新日期:2020-06-15 00:00:00

  • Cloning, expression and characterization of β- and γ‑carbonic anhydrase from Bacillus sp. SS105 for biomimetic sequestration of CO2.

    abstract::Bacterium Bacillus sp. SS105, isolated from Free Air CO2 Enriched (FACE) soil was previously screened for carbonic anhydrase activity and CO2 sequestration. In this study, strain was selected to amplify carbonic anhydrase encoding genes. The CA genes from Bacillus sp. SS105 were found to be homologous with beta‑carbon...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.03.082

    authors: Maheshwari N,Kumar M,Thakur IS,Srivastava S

    更新日期:2019-06-15 00:00:00

  • alpha-Crystallin polymers and polymerization: the view from down under.

    abstract::Several models have been proposed for the quaternary structure of alpha-crystallin. Some suggest the subunits are arranged in concentric shells. Others propose that the subunits are in a micelle-like arrangement. However, none is able to satisfactorily account for all observations on the protein and the quaternary str...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/s0141-8130(98)00023-3

    authors: Augusteyn RC

    更新日期:1998-05-01 00:00:00

  • 3D composites based on the blends of chitosan and collagen with the addition of hyaluronic acid.

    abstract::3D porous composites based on blends of chitosan, collagen and hyaluronic acid were obtained through the lyophilization process. Mechanical properties, swelling behavior and thermal stability of the blends were studied. Moreover, SEM images were taken and the structure of the blends was studied. Biological properties ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.04.085

    authors: Sionkowska A,Kaczmarek B,Lewandowska K,Grabska S,Pokrywczyńska M,Kloskowski T,Drewa T

    更新日期:2016-08-01 00:00:00

  • Sequence-specific B-to-Z transition in self-assembled DNA: A biophysical and thermodynamic study.

    abstract::The most remarkable conformational transition in nature is the B-to-Z transition of DNA which not only contributes for epigenetic regulation but also is exploited to create several advanced nanomaterials for sensing and nanomechanics. The present communication focuses on the intrinsic factors that control the La3+/Ce3...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.06.166

    authors: Bhanjadeo MM,Baral B,Subudhi U

    更新日期:2019-09-15 00:00:00

  • Heparin based polyurethanes: A state-of-the-art review.

    abstract::Polyurethanes (PUs) are considered currently as one of the established bio compatible and blood compatible biomaterials offering tremendous structure-property relationship. But few limitations such as low resistance to micro-emboli and thrombi are still associated with these biomaterials that restricted their applicat...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审

    doi:10.1016/j.ijbiomac.2015.12.004

    authors: Zia F,Zia KM,Zuber M,Tabasum S,Rehman S

    更新日期:2016-03-01 00:00:00

  • Injectable pullulan hydrogel for the prevention of postoperative tissue adhesion.

    abstract::Methods for reducing and preventing postoperative abdominal adhesions have been researched for decades; however, despite these efforts, the formation of postoperative peritoneal adhesions is continuously reported. Adhesions cause serious complications such as postoperative pain, intestinal obstruction, and infertility...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.02.026

    authors: Bang S,Lee E,Ko YG,Kim WI,Kwon OH

    更新日期:2016-06-01 00:00:00

  • A structural study on the protection of glycation of superoxide dismutase by thymoquinone.

    abstract::Accumulation of advanced glycation end products (AGEs) in tissues and serum plays important roles in diabetes-associated complications. Therefore, the identification of antiglycating compounds is attracting considerable interest. In this study, the structural changes associated with the glycation of superoxide dismuta...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2014.06.003

    authors: Anwar S,Khan MA,Sadaf A,Younus H

    更新日期:2014-08-01 00:00:00

  • Physiometabolic effects of Agave salmiana fructans evaluated in Wistar rats.

    abstract::A prospective completely randomized experimental study was conducted using 48 animals to evaluate the physiometabolic effects of Agave salmiana fructans as a dietary supplement in healthy Wistar rats. Five fructans concentrations from 5 to 20% (w/w) and one control were used in the rats' diet and were divided into six...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.11.043

    authors: Castillo Andrade AI,Rivera Bautista C,Godínez Hernández C,Ruiz Cabrera MA,Fuentes Ahumada C,García Chávez E,Grajales Lagunes A

    更新日期:2018-03-01 00:00:00

  • Properties and behavior under environmental factors of isosorbide-plasticized starch reinforced with microcrystalline cellulose biocomposites.

    abstract::Green biocomposites based on corn starch plasticized with isosorbide and glycerol and filled with microcrystalline cellulose (MCC) were processed. The structural interactions, dynamomechanical properties, water absorption, and soil biodegradability were investigated by different techniques considering the effect of th...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.08.075

    authors: Area MR,Montero B,Rico M,Barral L,Bouza R,López J

    更新日期:2020-12-01 00:00:00

  • Effect of solution plasma process with bubbling gas on physicochemical properties of chitosan.

    abstract::In the present work, solution plasma process (SPP) with bubbling gas was used to prepare oligochitosan. The effect of SPP irradiation with bubbling gas on the degradation of chitosan was evaluated by the intrinsic viscosity reduction rate and the degradation kinetic. The formation of OH radical was studied. Changes of...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.01.049

    authors: Ma F,Li P,Zhang B,Zhao X,Fu Q,Wang Z,Gu C

    更新日期:2017-05-01 00:00:00

  • Alkaline proteases from a newly isolated Micromonospora chaiyaphumensis S103: Characterization and application as a detergent additive and for chitin extraction from shrimp shell waste.

    abstract::The present study was undertaken to characterize the extracellular thermostable serine alkaline proteases from newly actinomycete strain Micromonospora chaiyaphumensis S103 and to describe their evaluation in commercial detergents and shrimp waste deproteinization. This proteolytic crude extract was active and stable ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.10.036

    authors: Mhamdi S,Ktari N,Hajji S,Nasri M,Sellami Kamoun A

    更新日期:2017-01-01 00:00:00

  • Exploration of DNA binding mode, chemical nuclease, cytotoxic and apoptotic potentials of diketone based oxovanadium(IV) complexes.

    abstract::Two diketone based oxovanadium complexes, viz., bis(4,4,4-trifluoro-1-phenylbutane-1,3-dionato)oxovanadium(IV) (1) and bis(1,1,1-trifluoropentane-2,4-dionato)oxovanadium(IV) (2), have been synthesized and characterized by spectroscopic and analytical techniques. The DNA binding and the cleaving ability of the complexe...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.02.027

    authors: Inamdar PR,Sheela A

    更新日期:2015-05-01 00:00:00

  • Light-induced phosphorylation of crystallins in the retinal pigment epithelium.

    abstract::Protein phosphorylations have essential regulatory roles in visual signaling. Previously, we found that phosphorylation of several proteins in the retina and retinal pigment epithelium (RPE) is involved in anti-apoptotic signaling under oxidative stress conditions, including light exposure. In this study, we used a ph...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2010.11.006

    authors: Lee H,Chung H,Lee SH,Jahng WJ

    更新日期:2011-01-01 00:00:00

  • Effect of Ca(2+) on Aß40 fibrillation is characteristically different.

    abstract::Alzheimer's disease (AD) is the only one among top ten diseases in USA that cannot be cured, prevented or slowed down. At molecular level the mechanism of onset has been closely associated with mis-folding of Aβ40 and Aβ42 and is well supported by the genetic data for AD. Extensive research efforts have led to identif...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.04.082

    authors: Ahmad A,Stratton CM,Scemama JL,Muzaffar M

    更新日期:2016-08-01 00:00:00

  • Inhibition of methemoglobin formation in aqueous solutions under aerobic conditions by the addition of amino acids.

    abstract::Hemoglobin (Hb) as an important iron-containing oxygen-transport protein is easily oxidized to the ferric met-form, methemoglobin (metHb), and loses the capacity of binding oxygen during storage. In this study, the experimental data indicate that the presence of Tyr and Glu significantly suppress the metHb formation i...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2013.12.010

    authors: Wei Y,Li C,Zhang L,Su Z,Xu X

    更新日期:2014-03-01 00:00:00

  • Characterization of a d-tagatose 3-epimerase from Caballeronia fortuita and its application in rare sugar production.

    abstract::Recently, rare sugars have caused extensively attention due to their beneficial physiological functions and potential applications in food systems and medical fields. Ketose 3-epimerase (KEase) can catalyze reversibly the epimerization between ketoses which is the pivotal enzyme in Izumoring strategy and an effective ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.07.112

    authors: Li S,Chen Z,Zhang W,Guang C,Mu W

    更新日期:2019-10-01 00:00:00

  • Chromium (VI) detoxification by oxidation and flocculation of exopolysaccharides from Arthrobacter sp. B4.

    abstract::The exopolysaccharides from Arthrobacter sp. B4 (B4-EPS) exhibited an excellent chromium (VI) (Cr(VI)) removal capability without any pH adjustment, whereby 50mgL(-1) of Cr(VI) could be completely removed by 4gL(-1) of B4-EPS. The kinetics tests revealed that the first-order rate constant was 8.3×10(-5)s(-1) and the o...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.07.013

    authors: Li Y,Li Q,Fengying Y,Bao J,Hu Z,Zhu W,Zhao Y,Lin Z,Dong Q

    更新日期:2015-11-01 00:00:00

  • Hirudin protects against isoproternol-induced myocardial infraction by alleviating oxidative via an Nrf2 dependent manner.

    abstract::Oxidative stress plays a critical role in the progression of myocardial injury. Increasing evidence suggests that hiruidin can treat patients with cardio-injury. However, the mechanism of hirudin against myocardial infraction remains unknown. In the present study, we evaluated the potential role and mechanism of hirud...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.06.097

    authors: Zhang H,Chen H,Li J,Bian Y,Song Y,Li Z,He F,Liu S,Tsai Y

    更新日期:2020-11-01 00:00:00

  • Observation of in vitro cellulose synthesis by bacterial cellulose synthase with time-resolved small angle X-ray scattering.

    abstract::Cellulose synthase is the enzyme that produces cellulose in the living organisms like plant, and has two functions: polymerizing glucose residues (polymerization) and assembling these polymerized molecules into a crystalline microfibril with a "cellulose I" crystallographic structure (crystallization). Many studies, h...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.02.167

    authors: Tajima H,Penttilä PA,Imai T,Yamamoto K,Yuguchi Y

    更新日期:2019-06-01 00:00:00

  • Investigation of inulins from the roots of Morinda officinalis for potential therapeutic application as anti-osteoporosis agent.

    abstract:SCOPE:In China, the root of M. officinalis has been widely used over thousands of years against a wide range of bone disease such as lumbago, limb-ache, sciatica and rheumatic arthralgia, and has tremendous medicinal value. But the bioactive constituents responsible for the osteoprotective effects in M. officinalis rem...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.08.082

    authors: Jiang K,Huang D,Zhang D,Wang X,Cao H,Zhang Q,Yan C

    更新日期:2018-12-01 00:00:00

  • Extraction and characterization of gelatin from the feet of Pekin duck (Anas platyrhynchos domestica) as affected by acid, alkaline, and enzyme pretreatment.

    abstract::The effects of different pretreatments on yield and composition of extraction, physicochemical, and rheological properties of duck feet gelatin (DFG) were investigated. Gelatins were extracted from the whole feet of Pekin duck with an average yield of 4.09%, 3.65%, and 5.75% for acidic (Ac-DFG), alkaline (Al-DFG), and...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.01.139

    authors: Abedinia A,Ariffin F,Huda N,Nafchi AM

    更新日期:2017-05-01 00:00:00

  • Structural and thermodynamic properties of rice starches with different genetic background Part 1. Differentiation of amylopectin and amylose defects.

    abstract::A combined DSC - HPAEC-PAD approach, gel permeation chromatography and mild long-term acidic hydrolysis were employed to study the effects of amylopectin chain-length distributional and amylose defects on the assembly structures of amylopectin (crystalline lamellae, amylopectin clusters) in A-type polymorphic starches...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2007.05.010

    authors: Koroteeva DA,Kiseleva VI,Sriroth K,Piyachomkwan K,Bertoft E,Yuryev PV,Yuryev VP

    更新日期:2007-10-01 00:00:00

  • Effects of electric field on the maximum electro-spinning rate of silk fibroin solutions.

    abstract::Owing to the excellent cyto-compatibility of silk fibroin (SF) and the simple fabrication of nano-fibrous webs, electro-spun SF webs have attracted much research attention in numerous biomedical fields. Because the production rate of electro-spun webs is strongly dependent on the electro-spinning rate used, the electr...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.11.026

    authors: Park BK,Um IC

    更新日期:2017-02-01 00:00:00

  • Physicochemical functionality of 4-α-glucanotransferase-treated rice flour in food application.

    abstract::The physicochemical properties of 4-α-glucanotransferase (4αGTase)-modified rice flours were examined by measuring the molecular weight distribution, moisture sorption isotherm, and melting enthalpy of ice crystals. The results obtained by measuring the moisture sorption isotherm and melting enthalpy of ice crystals r...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2013.04.032

    authors: Kim YL,Mun S,Park KH,Shim JY,Kim YR

    更新日期:2013-09-01 00:00:00

  • Inhibition of amyloid fibril formation and cytotoxicity by a chemical analog of Curcumin as a stable inhibitor.

    abstract::Clinical application of curcumin for Alzheimer's disease treatment is severely limited with regard to its poor bioavailability, high rate of metabolism, and instability under neutral condition. In the current study, we designed three compounds in which the diketone moiety of curcumin was replaced by cyclohexanone. In ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.04.038

    authors: Ramshini H,mohammad-zadeh M,Ebrahim-Habibi A

    更新日期:2015-01-01 00:00:00

  • Molecular cloning, prokaryotic expression, purification, structural studies and functional implications of Heat Shock Protein 70 (Hsp70) from Rutilus frisii kutum.

    abstract::A novel Hsp70 chaperone from Rutilus frisii kutum was identified, cloned, expressed, purified and its functional characteristics revealed. The 3D structure of Hsp70 from Rutilus kutum was constructed using the crystal structure of E. coli Hsp70 as the template, with 47% sequence identity. The in vitro ATPase activity ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.10.174

    authors: Jahangirizadeh Z,Ghafouri H,Sajedi RH,Sarikhan S,Taghdir M,Sariri R

    更新日期:2018-03-01 00:00:00