Abstract:
:Double hydrophilic thermo-responsive pullulan-g-poly(N-isopropylacrylamide) (P-g-pNIPAM) copolymers with two different molecular weight of thermosensitive grafts were synthesized and used for preparation of indomethacin-loaded nanoparticles by dialysis and nanoprecipitation method. The polymers form aggregates in aqueous solution at a concentration of 10g/L, above their critical aggregation concentration (3.36g/L) and below the lower critical solution temperature (LCST). After indomethacin loading, nanoparticles with compact and uniform structure were formed below the LCST. The effects of copolymer composition, concentration, and the feed polymer/drug ratio on the particle size, drug loading content (DLC) and entrapment efficiency (EE) were investigated. DLC increased with drug feeding, reaching a maximum value of 40% at the ratios of 1/1. Smaller particles (145nm) with narrower size distribution were obtained from polymer with a higher molecular weight of pNIPAM grafts. FT-IR and 1H NMR spectra proved that the main driven force for the aggregation was the hydrogen bonding between indomethacin and the pNIPAM side chains of copolymer. The indomethacin release rate from nanoparticles was influenced by temperature, because of the dissociation of the hydrogen bonds at high temperatures, the degree of drug loading, and the pH of the release media.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Constantin M,Bucătariu S,Stoica I,Fundueanu Gdoi
10.1016/j.ijbiomac.2016.10.064subject
Has Abstractpub_date
2017-01-01 00:00:00pages
698-708issue
Pt Aeissn
0141-8130issn
1879-0003pii
S0141-8130(16)30823-6journal_volume
94pub_type
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