Abstract:
:From a set of mixed carbon sources, 5-phenylvaleric acid (PV) and octanoic acid (OA), polyhydroxyalkanoic acid (PHA) was separately accumulated in the two pseudomonads Pseudomonas putida BM01 and Pseudomonas citronellolis (ATCC 13674) to investigate any structural difference between the two PHA accumulated under a similar culture condition using one-step culture technique. The resulting polymers were isolated by chloroform solvent extraction and characterized by fractional precipitation and differential scanning calorimetry. The solvent fractionation analysis showed that the PHA synthesized by P. putida was separated into two fractions, 3-hydroxy-5-phenylvalerate (3HPV))-rich PHA fraction in the precipitate phase and 3-hydroxyoctanoate (3HO)-rich PHA fraction in the solution phase whereas the PHA produced by P. citronellolis exhibited a rather little compositional separation into the two phases. According to the thermal analysis, the P. putida PHA exhibited two glass transitions indicative of the PHA not being homogeneous whereas the P. citronellolis PHA exhibited only one glass transition. It was found that the structural heterogeneity of the P. putida PHA was caused by a significant difference in the assimilation rate between PV and OA. The structural heterogeneity present in the P. putida PHA was also confirmed by a first order degradation kinetics analysis of the PHA in the cells. The two different first-order degradation rate constants (k(1)), 0.087 and 0.015/h for 3HO- and 3HPV-unit, respectively, were observed in a polymer system over the first 20 h of degradation. In the later degradation period, the disappearance rate of 3HO-unit was calculated to be 0.020 h. The k(1) value of 0.083/h, almost the same as for the 3HO-unit in the P. putida PHA, was obtained for the P(3HO) accumulated in P. putida BM01 grown on OA as the only carbon source. In addition, the k(1) value of 0.015/h for the 3HPV-unit in the P. putida PHA, was also close to 0.019/h for the P(3HPV) homopolymer accumulated in P. putida BM01 grown on PV plus butyric acid. On the contrary, the k(1) values for the P. citronellolis PHA were determined to be 0.035 and 0.029/h for 3HO- and 3HPV-unit, respectively, thus these two relatively close values implying a random copolymer nature of the P. citronellolis PHA. In addition, the faster degradation of P(3HO) than P(3HPV) by the intracellular P. putida PHA depolymerase indicates that the enzyme is more specific against the aliphatic PHA than the aromatic PHA.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Chung DM,Choi MH,Song JJ,Yoon SC,Kang IK,Huh NEdoi
10.1016/s0141-8130(01)00172-6keywords:
subject
Has Abstractpub_date
2001-12-10 00:00:00pages
243-50issue
4-5eissn
0141-8130issn
1879-0003pii
S0141813001001726journal_volume
29pub_type
杂志文章abstract::The alpha chitin and chitosan used in the experiments came from crab shell waste (Sigma). This was treated to form a colloidal suspension of chitin or chitosan crystallites. The electrostatic 'charge coat' surrounding the chitin was then manipulated. This was achieved by alteration of the pH of the chitin or chitosan ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/s0141-8130(98)00002-6
更新日期:1998-04-01 00:00:00
abstract::Carboxymethyl cellulose (CMC)/pPoly-N-isopropylacrylamide (PNIPAM) hydrogels were prepared using nanoclay as physical cross-linker by UV irradiation. PNIPAM/CMC hydrogels exhibit 3D network structures with high porosity. PNIPAM/CMC hydrogels exhibit not only high water absorption capacity, but also temperature, pH and...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.06.094
更新日期:2020-10-15 00:00:00
abstract::In our earlier communication on urea denaturation of bovine serum albumin (BSA), we showed significant unfolding of domain III along with domain I prior to intermediate formation around 4.6-5.2 M urea based on the binding results of domain specific ligands:chloroform, bilirubin and diazepam for domains I, II and III, ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/s0141-8130(01)00185-4
更新日期:2002-03-08 00:00:00
abstract::Basa acellular dermal matrix (BADM) has advantages in the preparation of oral prosthetic membranes. In order to prepare high-quality BADM, a suitable cross-linking agent is necessary. In this study, acellular dermal matrix was prepared from basa fish skin and then cross-linked with carbodiimide (EDC), oxidized chitosa...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.07.019
更新日期:2020-12-01 00:00:00
abstract::A newly isolated bacterium producing 55.5 U/mL keratinase on feather meal minimal medium was identified as Ochrobactrum intermedium. Optimization of process parameters by one-variable-at-a-time (OVAT) approach (substrate concentration 0.5% w/v, inoculum size 5% w/v, pH 7.0, 200 rpm for 96 h at 40 °C) resulted in 2.1-f...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.10.260
更新日期:2021-01-01 00:00:00
abstract::In the present work, N,N,N-trimethyl chitosan (TMC) fibers were synthesized successfully and the resulting quaternized materials were characterized by FTIR. The designed TMC fibers with different degree of quaternization achieved high water absorption capability. In antibacterial activity study, TMC fibers showed high...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.02.036
更新日期:2016-08-01 00:00:00
abstract::In this study, a class of interpenetrating network (IPN) gels were successfully fabricated based on glucono-δ-lactone (GDL) induced gelation of mixed alginate (Alg) and deacylated gellan (gellan) systems. The IPN gels were prepared by co-hydrating a mixed Alg and gellan powder, followed by adding GDL and calcium carbo...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.02.107
更新日期:2020-05-15 00:00:00
abstract::Novel α-aminophosphonates 4 were synthesized via one pot three-component reaction of 4-aminoantipyrine, aldehydes, triphenylphosphite and Lewis acid catalyst. The chemical structures of all the synthesized compounds were elucidated by IR, NMR and MS spectral analysis. The antimicrobial activity of 4 was tested in vitr...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.06.251
更新日期:2020-11-15 00:00:00
abstract::In this paper, the binding of flavonoids (quercetin and rutin) to hemoglobin (Hb) have been investigated by fluorescence, absorption spectroscopy and circular dichroism (CD) spectroscopy. The binding parameters and binding mode between flavonoids and Hb are determined and the results of CD and synchronous fluorescence...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2006.08.011
更新日期:2007-03-10 00:00:00
abstract::Aggregative protein-polyelectrolyte complex (PPC) has been proposed as a concentrated state of protein with a great potential for biopharmaceutical application. In this review article, we introduce a unique concentration method of protein formulation using PPC for a dozen types of pharmaceutical antibodies, hormones, ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
doi:10.1016/j.ijbiomac.2016.06.016
更新日期:2017-07-01 00:00:00
abstract::Tremella fuciformis is an important edible mushroom that has been widely cultivated and used as food and medicinal ingredient in traditional Chinese medicine. In the past decades, many researchers have reported that T. fuciformis polysaccharides (TPS) possess various bioactivities, including anti-tumor, immunomodulato...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
doi:10.1016/j.ijbiomac.2018.10.117
更新日期:2019-01-01 00:00:00
abstract::A novel GH1 β-glucosidase (EaBgl1A) from a bacterium isolated from Antarctica soil samples was recombinantly overexpressed in Escherichia coli cells and characterized. The enzyme showed unusual pH dependence with maximum activity at neutral pH and retention of high catalytic activity in the pH range 6 to 9, indicating...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.09.018
更新日期:2016-01-01 00:00:00
abstract::The aim a present study was developed methylcellulose (MC) active edible films with extracts of green apple skin, as model systems of edible coating. Active edible films were developed by incorporation of ethanolic extract of freeze-dried apple skin (EEFD) and aqueous extract of apple skin (AES) at 10, 20 and 25% (v/v...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.12.114
更新日期:2019-03-01 00:00:00
abstract::In the food packaging sector many efforts have been (and are) devoted to the development of new materials in order to reply to an urgent market demand for green and eco-sustainable products. Particularly a lot of attention is currently devoted both to the use of compostable and biobased polymers as innovative and prom...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.02.018
更新日期:2018-06-01 00:00:00
abstract::This study investigated the effect of Lactobacillus fermentum fermentation treatment on the gastrointestinal digestion and fermentation in vitro of polysaccharides from longan pulp. Polysaccharide isolated from unfermented and fermented longan pulp named LP and LP-F, respectively. The molecular weight of LP-F declined...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.01.059
更新日期:2020-03-15 00:00:00
abstract::1,4-α-Glucan branching enzyme (GBE, EC. 2.4.1.18), which plays a key role in the synthesis of starch and glycogen, has been overexpressed in E. coli as an intracellular enzyme by many researchers. In this study, it was found that the GBEs from Geobacillus thermoglucosidans and Rhodothermus obamensis were secreted into...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.04.002
更新日期:2019-07-01 00:00:00
abstract::Selenium-enriched polysaccharides have become a major research focus owing to their high anti-oxidant activities. Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium. ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.12.152
更新日期:2018-05-01 00:00:00
abstract::A new class of sequential oligopeptide carriers (SOCn), namely (Lys-Aib-Gly)n (n = 2-7), for anchoring antigenic peptides, is presented. These SOCn have been designed in order to assume a determined structural motif, exhibiting defined spatial orientations of the Lys-N epsilon H2 anchoring groups. The NMR study showed...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/0141-8130(96)01128-2
更新日期:1996-10-01 00:00:00
abstract::In this study, the complete mitochondrial DNA (mtDNA) sequence of the crab Parasesarma affine is determined, characterized and compared with other decapod crustaceans. The P. affine mitochondrial genome (mitogenome) is 15,638 bp in size, and contains 13 protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 tra...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.06.056
更新日期:2018-10-15 00:00:00
abstract::Trace elements regulate many biological reactions in the body. Copper(II) is known as one of trace elements and capable of binding to proteins. Insulin is a blood glucose-lowering peptide hormone and it is secreted by the pancreatic β-cells. In this study, Cu(II)-insulin complexes were investigated by using ESI-MS met...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.01.150
更新日期:2018-06-01 00:00:00
abstract::EPPS, a new heteropolysaccharide from Echinops pungens roots, was extracted by warm water (70 °C) and fortuitously purified using column chromatographies on DEAE-cellulose A52 and Sephadex G-100. Total carbohydrate content, specific optical rotation and molecular mass of EPPS were 98.3%, -88.2 and 65.7 kDa, respective...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.06.128
更新日期:2020-10-15 00:00:00
abstract::Glycosylated flagellin of the polar flagellum of the plant growth-promoting rhizobacteria Azospirillum brasilense Sp245 was for the first time isolated and characterized by biochemical and bioinformatics methods. Using the amino acid sequence taken from the NCBI database of bacterial whole-genome DNA sequencing, the s...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.10.092
更新日期:2020-03-15 00:00:00
abstract::In this study, ginger polysaccharide (GP) was obtained from ginger by enzymatic method, its chemical properties and antitumor activity were investigated. The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.10.169
更新日期:2019-02-15 00:00:00
abstract::Dendrobium Taiseed Tosnobile, a new Dendrobium species developed by crossbreeding Dendrobium tosaense and Dendrobium nobile, exhibits the characteristics of high mass production and high polysaccharide content. This study investigated the structural characterization and immunostimulating effects of a polysaccharide is...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.05.185
更新日期:2017-10-01 00:00:00
abstract::Two guest molecules (eugenol and (-)-menthol) were investigated on inclusion complex formation with water-soluble quaternized β-CD grafted with chitosan (QCD-g-CS). The inclusion complexes were prepared at varying mole ratios between eugenol or (-)-menthol and β-CD (substituted on QCD-g-CS) by a conventional shaking m...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.11.006
更新日期:2016-03-01 00:00:00
abstract::Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods. Structural analyses of these purified polysaccharides indicated that they were ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.06.177
更新日期:2019-09-15 00:00:00
abstract::Myeloid-derived suppressor cells (MDSCs) accumulate in tumor-bearing hosts and play a major role in tumor-induced immunosuppression. The potent modulatory effects of polysaccharides on the innate and adaptive immune system stimulate antitumor responses. In this study, a polysaccharide with an apparent molecular weight...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.04.153
更新日期:2018-08-01 00:00:00
abstract::An improved amperometric biosensor was fabricated by immobilizing glycerol kinase (GK) and glycerol-3-phosphate oxidase (GPO) nanoparticles (NPs) onto graphene oxide nanoparticles (GrONPs) modified pencil graphite (PG) electrode. The GKNPs, GPONPs and GrONPs were characterized by UV spectroscopy, and transmission elec...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.12.253
更新日期:2019-04-15 00:00:00
abstract::The present study deals with development and evaluation of the safety profile of chitosan/alginate nanoparticles as a platform for delivery of a natural antioxidant quercetin. The nanoparticles were prepared by varying the ratios between both biopolymers giving different size and charge of the formulations. The biocom...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.05.062
更新日期:2017-10-01 00:00:00
abstract::Therapeutic value of allelochemicals in inflammatory disorders and the potential drug targets need to be elucidated to alleviate tissue and vascular injury. Natural anti-inflammatory agents are known to cause minimal adverse effects. Presence of different secondary metabolites (allelochemicals), protease inhibitors li...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.03.139
更新日期:2018-07-15 00:00:00