Abstract:
:Aggregative protein-polyelectrolyte complex (PPC) has been proposed as a concentrated state of protein with a great potential for biopharmaceutical application. In this review article, we introduce a unique concentration method of protein formulation using PPC for a dozen types of pharmaceutical antibodies, hormones, and enzymes. Aggregative PPC can be obtained only by mixing poly(amino acid)s with proteins under low salt concentration conditions at an ambient temperature. The aggregative PPC is in a stabilized state against shaking, heating, and oxidation. More importantly, the aggregative PPC can be fully redissolved by the addition of physiological saline without denaturation and activity loss for many proteins. In addition, the general toxicity and pharmacokinetic profiles of the aggregative PPC are identical to those of the control antibody formulation. Thus, the protein formulation produced by aggregative PPC would be applicable for biomedical use as a kind of concentrated-state protein.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Kurinomaru T,Shiraki Kdoi
10.1016/j.ijbiomac.2016.06.016subject
Has Abstractpub_date
2017-07-01 00:00:00pages
11-17eissn
0141-8130issn
1879-0003pii
S0141-8130(16)30542-6journal_volume
100pub_type
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