Abstract:
:Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30-50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods. Structural analyses of these purified polysaccharides indicated that they were all branched, with a degree of branching (DB) ranging from 0.2 to 0.4. The polysaccharides exhibited strong scavenging activities for 2,2‑diphenyl‑1‑picrylhydrazyl and hydroxyl radicals. Their antioxidant activities were correlated to some extent with their composition but significantly with the DB. The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2-0.3) had higher antioxidant activities. Additionally, the correlation between the extraction yield and antioxidant activities of the polysaccharides was assessed. The low-MW polysaccharides extracted by alkali treatments had both high yield and antioxidant activities. Additionally, both the yield and antioxidant activities were found to be influenced by the size, extraction method, and source (fermentation liquor, mycelia, or basidiomata) of the polysaccharides. These results indicate that polysaccharides isolated from S. latifolia have strong antioxidant activities closely associated with the polysaccharide structure and extraction method, thus providing a scientific basis for further use of these polysaccharides.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Duan GL,Yu XBdoi
10.1016/j.ijbiomac.2019.06.177subject
Has Abstractpub_date
2019-09-15 00:00:00pages
1112-1120eissn
0141-8130issn
1879-0003pii
S0141-8130(19)30819-0journal_volume
137pub_type
杂志文章abstract::Functional and conformational transitions of the Sophora japonica seed lectin (SJL) were studied in detail using bioinformatics and biophysical tools. Homology model of the lectin displayed all the characteristics of the legume lectin monomer and the experimental observations correlated well with the structural inform...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.05.047
更新日期:2016-10-01 00:00:00
abstract::The polar microenvironment around the reactive Cys283 of rabbit muscle creatine kinase was explored using kinetic analysis of substrates reaction in the presence of modifiers. In the present study, three specific sulphydryl reagents, 5,5'-dithiobis(2-nitrobenzoic acid), 6,6'-dithiodinicotinic acid and 2,2'-dithiodipyr...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2007.05.004
更新日期:2007-10-01 00:00:00
abstract::This work aimed to study the potential of 155 strains of Bacillus sp., isolated from a collection of Tunisian microorganisms, for polyhydroxyalcanoates production. The strains were submitted to a battery of standard tests commonly used for determining bioplastic properties. The findings revealed that two of the isolat...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2013.06.043
更新日期:2013-10-01 00:00:00
abstract::Microcrystalline cellulose and Bombyx mori silk blended biocomposite films were regenerated using various imidazolium-based ionic liquids. The films were characterized to understand the effect of the inter- and intra-molecular interactions upon the morphology and thermal properties. Various techniques were implemented...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.11.137
更新日期:2018-03-01 00:00:00
abstract::Lesser usages of native starch has attracted attention in the modification of starch to incorporate its benefits for pharmaceutical application. A work has been carried out to study the influence of carboxymethylation on the morphological, physico-chemical, powder, spectroscopical and drug discharge characteristics of...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.07.039
更新日期:2016-11-01 00:00:00
abstract::Overconsumption of fat-rich foods has caused increasing incidences of human chronic diseases. Thus, it is promising to fabricate reduced-fat foods, but the reduction in fat content often causes undesired textural changes in foods. In this study, we showed a facile method to structure oil-in-water emulsions via the ele...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.01.106
更新日期:2020-03-15 00:00:00
abstract::A neutral polysaccharide, SPW-2, was purified from the leaves of Sambucus adnata Wall. using water extraction and alcohol precipitation, Sevage deproteination, ion exchange chromatography and gel filtration chromatography. This molecule had an average molecular weight of 7040 Da and was composed of arabinose, xylose, ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.11.021
更新日期:2020-07-01 00:00:00
abstract::The present study deals with mass spectrometric investigation to characterize the nature of proinsulin in inclusion bodies. Various derivatives of human proinsulin were cloned, expressed in E. coli and inclusion bodies prepared under weak acidic conditions (pH 6.5), which protected the native thiols. Non-reductive PAG...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.07.131
更新日期:2019-10-15 00:00:00
abstract::We report the preparation of both control chitosan and magnetic chitosan beads as biosorbents using chitosan as matrix and magnetite (Fe3O4) nanoparticles as reinforcement followed by detailed advanced characterization. The batch trials were performed to study the adsorption kinetics of biosorbents by removing As(III)...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.06.287
更新日期:2020-11-15 00:00:00
abstract::An effective antibacterial nanofiber film was prepared through the incorporation of ε-polylysine (ε-PL) into gelatin/chitosan-based polymers. All nanofiber films had uniformly disordered fibrous structure with good diameter distribution. The weight ratio of the gelatin/chitosan/ε-PL (G/C/P) influenced the solution pro...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.152
更新日期:2020-12-01 00:00:00
abstract::Pumpkin polysaccharide was extracted by hot water extraction method. It was modified with chlorosulfonic acid-pyridine to obtain sulfated pumpkin polysaccharides (SP1, SP2) with different degrees of substitution, which was 0.35 and 0.65, respectively. The total sugar contents of pumpkin polysaccharide and its sulfated...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.12.261
更新日期:2019-04-01 00:00:00
abstract::The water-soluble crude polysaccharides, extracted from the rhizome of Smilax glabra, were fractionated using an anion exchange chromatography, yielding two fractions, F1 and F2. The crude and fractions (F1 and F2) mainly consisted of carbohydrates (66.7%-91.1%), proteins (7.30%-23.9%) and minor amount of sulfates (1....
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.09.138
更新日期:2018-12-01 00:00:00
abstract::A novel GH1 β-glucosidase (EaBgl1A) from a bacterium isolated from Antarctica soil samples was recombinantly overexpressed in Escherichia coli cells and characterized. The enzyme showed unusual pH dependence with maximum activity at neutral pH and retention of high catalytic activity in the pH range 6 to 9, indicating...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.09.018
更新日期:2016-01-01 00:00:00
abstract::Silk sericin (SS) possessing moisture-retaining property was reacted with activated poly(ethylene glycol) (PEG) to obtain self-assembled SS nanoparticles. The aliphatic and aromatic hydroxyl groups of serine and tyrosine residues as the reaction sites in SS were clarified by amino acid analysis and 1H NMR spectroscopy...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/s0141-8130(03)00023-0
更新日期:2003-03-01 00:00:00
abstract::A putative cDNA of α-l-rhamnosidase was PCR-cloned from Aspergillus niger JMU-TS528 and further extracellular over-expressed in Pichia pastoris GS115. The activity of the recombinant α-l-rhamnosidase r-Rha1 was 711.9U/mL, eightfold higher than the native α-l-rhamnosidase from A. niger JMU-TS528. r-Rha1 is a N-glycosyl...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.12.093
更新日期:2016-04-01 00:00:00
abstract::Modified starches play a crucial role in the pharmaceutical industries in controlling the drug release at a pre-determined rate. The effect of acetylation on the physicochemical and drug release characteristics of the starches from five different Indian L. siceraria cultivars was investigated. Starches isolated from t...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2014.10.007
更新日期:2015-01-01 00:00:00
abstract::Pullulan (PU) is an exo-polysaccharide used in the food and pharmaceutical industries. However, the use of PU in different industries is limited due to its highly hydrophilic nature and consequently weakness in surface properties which can be remedied by its chemical modification with octenyl succinic anhydride (OSA)....
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.158
更新日期:2020-12-01 00:00:00
abstract::Silk fibroin (SF), is a very attractive protein-polymer, being processed into a variety of formats to match structural and morphological features for specific biomedical applications. The aim of the present work is to investigate blood compatibility of two forms, films and scaffolds, of silk fibroin-N,N' methylene bis...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.01.088
更新日期:2016-09-01 00:00:00
abstract::Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) was used to study the external regions of starch granules. Native starches (wheat, potato, maize, waxy maize and amylomaize) were analysed and compared to gelatinised and acid-hydrolysed starches. The IR spectra of potato and amylomaize starches were c...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/s0141-8130(02)00067-3
更新日期:2002-12-20 00:00:00
abstract::Sodium alginate, a naturally occurring macromolecule, in its granular form and without any post-modification was found to be an efficient and recoverable bifunctional heterogeneous organocatalyst for the domino synthesis of various 2-amino-3-cyano-4H-pyran annulated derivatives through three-component condensation of ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.01.080
更新日期:2016-06-01 00:00:00
abstract::Cancer is a major health issue concerning to all of us. Current treatment options are still limited due to not-selective action. Encapsulation is contemplated as an innovative approach to address systemic toxicity and tumor resistance caused by traditional therapies, while increasing encapsulated compounds bioavailabi...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
doi:10.1016/j.ijbiomac.2020.12.161
更新日期:2021-02-15 00:00:00
abstract::It is of great clinical significance to design wound dressing materials with combined excellent wound healing properties and superior capability to suppress hypertrophic scar formation. This study aimed to examine if and how the cationicity of chitosan would affect the hypertrophic scar-related outcomes, through prepa...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.03.161
更新日期:2020-07-01 00:00:00
abstract::Time-dependent intermolecular sulphydryl-disulphide interchange involving beta-lactoglobulin adsorbed at the oil-water interface in n-tetradecane-in-water emulsions (10 wt% oil, 0.5 wt% protein, pH 7.0) has been investigated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). While only monome...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/0141-8130(91)90006-g
更新日期:1991-02-01 00:00:00
abstract::Novel ternary deep eutectic solvents (DESs) were designed to efficiently dissolve the lignin for synthesizing the lignin-containing rigid polyurethane foam (LRPUF). These ternary DESs were constituted by choline chloride (ChCl), two hydrogen bond donors - glycerol (Gly) and polyethylene glycol (PEG-400). The maximum s...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.07.153
更新日期:2020-12-01 00:00:00
abstract::The shear flow of hyaluronate solutions prepared from mixtures of hyaluronates with different molecular weights has been studied. This paper shows that prediction of the rheological behaviour of polymer mixtures at a given polymer concentration is possible knowing the intrinsic viscosity of the different mixed samples...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/0141-8130(94)90040-x
更新日期:1994-06-01 00:00:00
abstract::Creatine kinase (CK) is a key enzyme involved in intracellular energy homeostasis. The distinct tissue distribution of muscle CK (MMCK) and brain CK (BBCK) implies that they function under conditions facing dissimilar environmental stresses. We found that MMCK and BBCK were significantly different in their stability a...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2010.03.025
更新日期:2010-07-01 00:00:00
abstract::A novel approach in fabricating anionic inter-polymeric complex polysaccharide bio-platform with enhanced mechanical, structural and chemical properties for burn wound dressings. This approach implies a facile, insitu method to design soft nano-floral inter-polymeric complexes. In particular, sodium alginate (SA) and ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.04.157
更新日期:2020-08-15 00:00:00
abstract::The process of recombinant protein production in E. coli system is often hampered by the formation of insoluble aggregates. Human Dihydrofolate reductase (hDHFR), an enzyme involved in the synthesis of purine, thymidilate and several other amino acids like glycine, methionine and serine is highly aggregation prone. It...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.05.143
更新日期:2017-10-01 00:00:00
abstract::In this study, we aimed to optimize the cultural and nutritional conditions for protease production by Lysinibacillus fusiformis strain C250R in submerged fermentation process using statistical methodology. The most significant factors (gruel, wheat bran, yeast extract, and FeSO4) were identified by Plackett-Burman de...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.03.051
更新日期:2017-08-01 00:00:00
abstract::Antioxidant effects of lentinan on LPS induced inflammatory response in intestine of juvenile taimen were evaluated, and its prebiotic-like efficacy on intestinal microbiota was also investigated. The results showed that LPS decreased the activities of antioxidant enzymes and increased the expression levels of inflamm...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.09.121
更新日期:2019-01-01 00:00:00