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Purification and characterization of two polyhydroxyalcanoates from Bacillus cereus.

Abstract：

:This work aimed to study the potential of 155 strains of Bacillus sp., isolated from a collection of Tunisian microorganisms, for polyhydroxyalcanoates production. The strains were submitted to a battery of standard tests commonly used for determining bioplastic properties. The findings revealed that two of the isolates, namely Bacillus US 163 and US 177, provided red excitations at a wavelength of approximately 543 nm. The polyhydroxyalcanoates produced by the two strains were purified. Gas chromatography-mass spectroscopy (GC-MS), Fourier transformed infrared spectroscopy (FTIR), and gel permeation chromatography (GPC) were used to characterize the two biopolymers. Bacillus US 163 was noted to produce a poly methyl-3-hydroxy tetradecanoic acid (P-3HTD) with an average molecular weight of 455 kDa, a completely amorphous homopolymer without crystallinity. The US 177 strain produced a homopolymer of methyl-3-hydroxy octadecanoic acid (P3-HOD) with an average molecular weight of 555 kDa. Exhibiting the highest performance, US 163 and US 177 were submitted to 16S rRNA gene sequencing, and the results revealed that they belonged to the Bacillus cereus species. Overall, the findings indicated that the Bacilli from petroleum soil have a number of promising properties that make them promising candidates for bioplastic production.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Zribi-Maaloul E,Trabelsi I,Elleuch L,Chouayekh H,Ben Salah R

doi

10.1016/j.ijbiomac.2013.06.043

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

82-8

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(13)00371-1

journal_volume

61

pub_type

杂志文章

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