当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Understanding the adhesion mechanism of a mucin binding domain from Lactobacillus fermentum and its role in enteropathogen exclusion.

Understanding the adhesion mechanism of a mucin binding domain from Lactobacillus fermentum and its role in enteropathogen exclusion.

Abstract：

:Lactobacillus species possesses surface exposed Mucin Binding Protein (MucBP) which plays a role in adhesion to gastrointestinal mucin. MucBP contains one or more mucin binding domain (MBD), the functionality of which has yet not been characterized thoroughly. Here, we have characterized a 93-amino acid MBD (MBD93) of MucBP (LAF_0673) from Lactobacillus fermentum. Multiple sequence alignment of L. fermentum MBD93 exhibited ∼60% sequence homology with MBDs from other Lactobacillus species. Further, we cloned, expressed and purified MBD93 from Escherichia coli as N-terminal histidine-tagged protein (6X His-MBD93). The purified MBD93 was able to bind to mucin and showed strong affinity towards the terminally expressed mucin glycans viz. N-acetylgalactosamine (GalNAc), N-acetylglucosamine (GlcNAc), Galactose (Gal), and Sialic acid (N-acetylneuraminic acid; Neu5Ac). In silico experiments further confirmed the interaction between homology modeled MBD93 to mucin glycans through hydrogen-bonding with its surface amino acid residues Ser57, Pro58, Ile60, Tyr63 and Ala65. We also have demonstrated that MBD93 was able to inhibit the adhesion of enteric pathogens, including E. coli, Salmonella Paratyphi A, Shigella sonnei and Proteus vulgaris to mucin. Our results suggested that L. fermentum MBD93 is a functionally sufficient unit to act as an adhesin and to protect from invading enteric pathogens.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Chatterjee M,Pushkaran AC,Vasudevan AK,Menon KKN,Biswas R,Mohan CG

doi

10.1016/j.ijbiomac.2017.10.107

subject

Has Abstract

pub_date

2018-04-15 00:00:00

pages

598-607

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(17)33078-7

journal_volume

110

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Alginate-chitosan/hydroxyapatite polyelectrolyte complex porous scaffolds: preparation and characterization.

    abstract：:Porous scaffolds composed of alginate (AG) and chitosan (CS) were fabricated by combining the formation of polyelectrolyte complex (PEC) with freeze-drying. The AG scaffold was used as a framework with uniformly distributed and interconnected pore structure. The chitosan or chitosan/hydroxyapatite (CS/HA) composite so...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2009.11.004

    authors： Han J,Zhou Z,Yin R,Yang D,Nie J

    更新日期：2010-03-01 00:00:00

  • Novel platinum complexes as efficient G-quadruplex DNA binders and telomerase inhibitors.

    abstract：:Three 4-(1H-imidazo[4,5-f]-1,10-phenanthrolin-2-yl)phenol derivatives-based platinum (II) complexes have been synthesized, and their G-quadruplex DNAs-binding interactions, telomerase inhibition, antiproliferative activity, and cell cycle arrest were studied. Three complexes show the preference for stabilizing h-telo,...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.01.004

    authors： Wei C,Wen Y,Wang J

    更新日期：2013-04-01 00:00:00

  • Electrochemical, spectroscopic, and theoretical studies on the interaction between azathioprine and DNA.

    abstract：:Possible interaction between immunosuppressive drug, azathioprine, and calf thymus DNA was explored by cyclic voltammetry, spectrophotometry, competitive spectrofluorimetry, circular dichroism spectroscopy (CD), and viscosity measurements. Cyclic voltammetry showed negative shift in the reduction peak of azathioprine ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.08.025

    authors： Jalali F,Rasaee G

    更新日期：2015-11-01 00:00:00

  • Synthesis and characterization of carboxymethyl starch-g-polyacrylic acids and their properties as adsorbents for ammonia and phenol.

    abstract：:Cigarette smoke contains a lot of harmful chemicals which cause different diseases like cancer, heart disease, bronchitis and ulcer etc. Ammonia and phenol are among those chemicals which cause cancer, fibrosis, respiratory disorder and pneumonia. So, to remove ammonia and phenol from cigarette smoke, five different t...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.07.046

    authors： Haroon M,Yu H,Wang L,Ullah RS,Haq F,Teng L

    更新日期：2019-10-01 00:00:00

  • Chitosan coated synergistically engineered nanoemulsion of Ropinirole and nigella oil in the management of Parkinson's disease: Formulation perspective and In vitro and In vivo assessment.

    abstract：:The research presented aims at developing Ropinirole hydrochloride (RHCl) nanoemulsion (NE) with nigella oil for Parkinson's disease (PD). In silico study was done to explore interactions of ropinirole and thymoquinone at receptor site (TNF-α and NFK-β). Ropinirole and Thymoquinone forms a hydrogen bond with residue A...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.11.207

    authors： Nehal N,Nabi B,Rehman S,Pathak A,Iqubal A,Khan SA,Yar MS,Parvez S,Baboota S,Ali J

    更新日期：2021-01-15 00:00:00

  • In vivo and ex vivo EPR detection of spin-labelled ovalbumin in mice.

    abstract：:In this study, spin-labelled ovalbumin (SL-OVA), free or entrapped in liposomes, was administered to mice subcutaneously (s.c.) or intravenously (i.v.) with the aim to determine the conditions for pharmacokinetic studies of spin-labelled proteins by EPR and to measure the time course of SL-OVA distribution in vivo in ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2010.06.006

    authors： Abramović Z,Brgles M,Habjanec L,Tomasić J,Sentjurc M,Frkanec R

    更新日期：2010-10-01 00:00:00

  • Characterization of a novel oligomeric SGNH-arylesterase from Sinorhizobium meliloti 1021.

    abstract：:A novel oligomeric SGNH-arylesterase (Sm23) from Sinorhizobium meliloti 1021 was characterized using biochemical and biophysical methods. A sequence comparison of Sm23 with other SGNH members confirmed the presence of catalytic triad (Ser(10), Asp(187), and His(190)) and oxyanion holes (Ser(10)-Gly(50)-Asn(90)). The w...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2009.12.010

    authors： Hwang H,Kim S,Yoon S,Ryu Y,Lee SY,Kim TD

    更新日期：2010-03-01 00:00:00

  • A new GH13 α-glucosidase from alkaliphilic Bacillus pseudofirmus 703 with both exo-α-l, 4-glucosidase and oligo-l, 6-glucosidase activities toward amylopectin.

    abstract：:Debranching of (1, 6)-α-linkages in starch is of great significance as it is widely used in different industries. In recent years, identifying a single potential enzyme that could debranch (1, 6)-α-linkages and cleave (1, 4)-α-linkages brought great interest as amylopectin possessed both (1, 4)-α- and (1, 6)-α-linkage...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.03.129

    authors： Wang S,Wang Q,Zhou Y,Lu Z,Zhang G,Ma Y

    更新日期：2017-08-01 00:00:00

  • Inhibitory effect on HT-29 colon cancer cells of a water-soluble polysaccharide obtained from highland barley.

    abstract：:A water-soluble polysaccharide (BP-1) was obtained from highland barley (Hordeum vulgare L.) by hot water extraction and purification of sepharose column chromatography. BP-1 had an average molecular weight of about 6.7×104Da and was composed of glucose (Glc), xylose (Xyl), arabinose (Ara) and rhamnose (Rha) with a re...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.06.099

    authors： Cheng D,Zhang X,Meng M,Han L,Li C,Hou L,Qi W,Wang C

    更新日期：2016-11-01 00:00:00

  • Binding mechanism of kinase inhibitors to EGFR and T790M, L858R and L858R/T790M mutants through structural and energetic analysis.

    abstract：:Experimental studies have demonstrated that L858R mutation in the EGF receptor (EGFR) confers tumor sensitivity whereas T790M and L858R/T790M mutations cause resistance to tyrosine kinase inhibitors in patients with non-small cell lung cancer. Theoretical studies have been carried out to try to clarify the structural ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.07.042

    authors： Bello M

    更新日期：2018-10-15 00:00:00

  • Chitosan nanoparticle hydrogel based sebacoyl moiety with remarkable capability for metal ion removal from aqueous systems.

    abstract：:In this present study, a new modified chitosan hydrogel, Cts-SC NPs, was prepared from a one pot reaction of sebacoyl chloride (SC) with chitosan in the presence of 1% v/v glacial acetic acid and 1% w/v sodium tripolyphosphate (TPP) using an ionotropic gelation technique. The modified chitosan hydrogel, Cts-SC NPs, wa...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.10.198

    authors： Kandile NG,Mohamed HM

    更新日期：2019-02-01 00:00:00

  • Natural and synthetic polymers/bioceramics/bioactive compounds-mediated cell signalling in bone tissue engineering.

    abstract：:Bone is a highly integrative and dynamic tissue of the human body. It is continually remodeled by bone cells such as osteoblasts, osteoclasts. When a fraction of a bone is damaged or deformed, stem cells and bone cells under the influence of several signaling pathways regulate bone regeneration at the particular local...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2017.09.029

    authors： Rao SH,Harini B,Shadamarshan RPK,Balagangadharan K,Selvamurugan N

    更新日期：2018-04-15 00:00:00

  • The attenuation of HIV-1 Tat-induced neurotoxicity by Salvianic acid A and Danshen granule.

    abstract：:The neurotoxicity of HIV-1 Tat protein contributes significantly to the pathogenesis of HAND, and hence the attractive therapeutic strategies focusing on Tat-induced neurotoxicity are warranted. Salvia miltiorrhiza have been known to antioxidant property and neuroprotective effects. The Danshen granule is the pharmace...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.11.146

    authors： Bao D,Wang J,Liu J,Qin T,Liu H

    更新日期：2019-03-01 00:00:00

  • Effect of organic solvent on enzymatic degradation of cyclic PBS-based polymers by lipase N435.

    abstract：:Poly(butylene succinate-co-cyclohexane dimethanol succinate) (P(BS-co-CHDMS)) and poly(butylene succinate-co-butanediol cyclohexanedicarboxylic acid) (P(BS-co-BCHDA)) were catalytically degraded by Candida antarctica lipase Novozyme 435 (N435) in CHCl3 and THF. The results indicated that the degradation rate was P(BS-...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.06.216

    authors： Li CT,Zhang M,Weng YX,Zhao D

    更新日期：2019-09-15 00:00:00

  • Ultrafiltration isolation, hypoglycemic activity analysis and structural characterization of polysaccharides from Brasenia schreberi.

    abstract：:Brasenia schreberi (B. schreberi) is a rare and precious vegetable, which coat with a gelatinous mucilage. This study is aiming to investigate the relationship between structural characteristics and hypoglycemic activities of polysaccharides with different molecular weight (Mw) range. Three polysaccharides fractions, ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.05.129

    authors： Feng S,Luan D,Ning K,Shao P,Sun P

    更新日期：2019-08-15 00:00:00

  • Preparation of bioactive functional poly(lactic acid)/curcumin composite film for food packaging application.

    abstract：:Poly (lactic acid)-based functional films incorporated with curcumin have been prepared using a solution casting method. The PLA/curcumin composite film was flexible and highly transparent with bright yellow color. Curcumin was uniformly dispersed in the PLA matrix and showed excellent compatibility with PLA, as evide...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.08.094

    authors： Roy S,Rhim JW

    更新日期：2020-11-01 00:00:00

  • Characterization of raw and alkali treated new natural cellulosic fibers from Tridax procumbens.

    abstract：:The aim of this study is to investigate natural cellulosic fibers extracted from Tridax procumbens plants. The obtained fibers were alkali treated for their effective usage as reinforcement in composites. The physical, chemical, crystallinity, thermal, wettability and surface characteristics were analyzed for raw, and...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.12.056

    authors： Vijay R,Lenin Singaravelu D,Vinod A,Sanjay MR,Siengchin S,Jawaid M,Khan A,Parameswaranpillai J

    更新日期：2019-03-15 00:00:00

  • Preparation of a novel Grifola frondosa polysaccharide-chromium (III) complex and its hypoglycemic and hypolipidemic activities in high fat diet and streptozotocin-induced diabetic mice.

    abstract：:Polysaccharide from Grifola frondosa is one of the best metal-ion chelating agents because of its structural characteristics and excellent functional activities. In this study, we synthesized and characterized a novel Grifola frondosa polysaccharide-chromium (III) [GFP-Cr(III)] complex. Response surface methodology (R...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.03.042

    authors： Guo WL,Shi FF,Li L,Xu JX,Chen M,Wu L,Hong JL,Qian M,Bai WD,Liu B,Zhang YY,Ni L,Rao PF,Lv XC

    更新日期：2019-06-15 00:00:00

  • Improving properties of Hanji by coating chitosan-silver nanoparticle solution.

    abstract：:A chitosan-silver nanoparticle solution (CSNS) was applied as a coating material to Hanji (Korean traditional paper), and the properties of the coated paper were investigated as a function of the dilution ratio. The required CSNS was first prepared from AgNO3 (30mmol) by utilizing chitosan as a reducing and stabilizin...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.09.067

    authors： Jung J,Raghavendra GM,Kim D,Seo J

    更新日期：2016-12-01 00:00:00

  • Study the effect of trypsin enzyme activity on the screening of applying frontal affinity chromatography.

    abstract：:Frontal affinity chromatography (FAC) combined with enzyme has been widely used for drug screening. In this paper, the effect of target enzyme activity on screening of bioactive compounds was studied through applying FAC. Trypsin with different degree of inactivation were prepared as target enzyme by thermal denaturat...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.07.218

    authors： Qian J,Zhao C,Tong J,Jiang S,Zhang Z,Lu S,Guo H

    更新日期：2019-10-15 00:00:00

  • Multi-analysis of chemical transformations of lignin macromolecules from waterlogged archaeological wood.

    abstract：:A large number of archaeological wooden building poles have been excavated from the Hai Menkou site (Yunnan province, China). Lignin can be transformed and altered accompanied with significant loss of carbohydrates during this process. Elucidation of chemical and structural transformations of lignin is of primary impo...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.12.114

    authors： Xia Y,Chen TY,Wen JL,Zhao YL,Qiu J,Sun RC

    更新日期：2018-04-01 00:00:00

  • Characterization of disintegrated bacterial cellulose nanofibers/PVA bionanocomposites prepared via ultrasonication.

    abstract：:With the increasing demand for simple, efficient, environmentally friendly preparation methods to produce cellulose nanofibers for reinforcing a biodegradable film is increased, the role of nanofibers from the pure cellulose produced by bacteria becomes more important. This work characterized bacterial cellulose nanof...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.05.178

    authors： Abral H,Kadriadi,Mahardika M,Handayani D,Sugiarti E,Muslimin AN

    更新日期：2019-08-15 00:00:00

  • Improvement of chitosan solubility and bactericidity by synthesis of N-benzimidazole-O-acetyl-chitosan and its electrodeposition.

    abstract：:Chitosan solubility and its antibacterial activity have been improved first of all with a chemical synthesis of N-benzimidazole-O-acetyl-chitosan in acetic medium and foremost through a potentiodynamic electrodeposition of this derivative. An association of one benzimidazole and three acetyl moieties per two units of ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.02.121

    authors： Rahmouni N,Tahri W,Sbihi HM,Nehdi IA,Desbrieres J,Besbes-Hentati S

    更新日期：2018-07-01 00:00:00

  • Tantalizing role of p53 molecular pathways and its coherent medications in neurodegenerative diseases.

    abstract：:Neurodegenerative diseases are incongruous, commonly age-related disorders characterized by progressive neuronal loss, comprising the most prevalent being Alzheimer's disease, Parkinson's disease, and Huntington's disease. Perilous health states are anticipated following the neurodegeneration. Their etiology remains l...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2021.01.042

    authors： Talebi M,Talebi M,Kakouri E,Farkhondeh T,Pourbagher-Shahri AM,Tarantilis PA,Samarghandian S

    更新日期：2021-01-10 00:00:00

  • Purification, characterization and end product analysis of dextran degrading endodextranase from Bacillus licheniformis KIBGE-IB25.

    abstract：:Degradation of high molecular weight dextran for obtaining low molecular weight dextran is based on the hydrolysis using chemical and enzymatic methods. Current research study focused on production, purification and characterization of dextranase from a newly isolated strain of Bacillus licheniformis KIBGE-IB25. Dextr...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.04.007

    authors： Zohra RR,Aman A,Ansari A,Haider MS,Qader SA

    更新日期：2015-07-01 00:00:00

  • Physicochemical properties of a high molecular weight levan from Brenneria sp. EniD312.

    abstract：:A high molecular weight levan was produced by a novel levansucrase and some properties of this polymer were investigated. The levan exhibited a poroid microstructure as well as series of individual ellipsoidal or spheroidal particles. The weight-average molecular weight (M¯w) of the levan was determined to be 1.41×108...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.11.056

    authors： Xu W,Liu Q,Bai Y,Yu S,Zhang T,Jiang B,Mu W

    更新日期：2018-04-01 00:00:00

  • Time-dependent polymerization of beta-lactoglobulin through disulphide bonds at the oil-water interface in emulsions.

    abstract：:Time-dependent intermolecular sulphydryl-disulphide interchange involving beta-lactoglobulin adsorbed at the oil-water interface in n-tetradecane-in-water emulsions (10 wt% oil, 0.5 wt% protein, pH 7.0) has been investigated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). While only monome...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/0141-8130(91)90006-g

    authors： Dickinson E,Matsumura Y

    更新日期：1991-02-01 00:00:00

  • Bioconversion of crude glycerol to polyhydroxyalkanoate by Bacillus thuringiensis under non-limiting nitrogen conditions.

    abstract：:Glycerol has emerged as a cheap waste material due to blooming biodiesel manufacturing units worldwide. The need is to exploit the crude glycerol (CG) to produce useful products such as polyhydroxyalkanoate (PHA). Bacillus thuringiensis EGU45 was found to produce 1.5-3.5 gP HA L(-1) from feed containing 1-10% CG (vv(-...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.03.046

    authors： Kumar P,Ray S,Patel SK,Lee JK,Kalia VC

    更新日期：2015-01-01 00:00:00

  • Modulation of interfacial phenolic antioxidant distribution in Pickering emulsions via interactions between zein nanoparticles and gallic acid.

    abstract：:The impacts of protein nanoparticles on the interfacial distribution of antioxidants and the oxidative stability in Pickering emulsions are attracting increasing research interests. In the present work, the distribution of gallic acid (GA) in zein nanoparticles-stabilized Pickering emulsions (ZPE) was determined by em...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.02.136

    authors： Zhao Z,Lu M,Mao Z,Xiao J,Huang Q,Lin X,Cao Y

    更新日期：2020-06-01 00:00:00

  • Ginsenoside Rb1 ameliorates autophagy via the AMPK/mTOR pathway in renal tubular epithelial cells in vitro and in vivo.

    abstract：:Although ginsenoside Rb1 (G-Rb1) has exerted an inhibitory effect on renal fibrosis and progression of chronic kidney disease (CKD), its mechanism remains unknown. This study aims to explore the anti-fibrosis effect of G-Rb1 in unilateral ureter obstruction (UUO) mouse model and underlying mechanisms in HBSS-induced H...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.07.060

    authors： Liu X,Chen J,Sun N,Li N,Zhang Z,Zheng T,Li Z

    更新日期：2020-11-15 00:00:00