Abstract:
:Degradation of high molecular weight dextran for obtaining low molecular weight dextran is based on the hydrolysis using chemical and enzymatic methods. Current research study focused on production, purification and characterization of dextranase from a newly isolated strain of Bacillus licheniformis KIBGE-IB25. Dextranase was purified up to 36 folds with specific activity of 1405 U/mg and molecular weight of 158 kDa. It was found that enzyme performs optimum cleavage of dextran (5000 Da, 0.5%) at 35 °C in 15 min at pH 4.5 with a Km and Vmax of 0.374 mg/ml and 182 μmol/min, respectively. Relative amino acid composition analysis of purified enzyme suggested the presence of higher number of hydrophobic, acidic and glycosylation promoting amino acids. The N-terminal sequence of dextranase KIBGE-IB25 was AYTVTLYLQG. It exhibited distinct amino acid sequence yet shared some inherent characteristics with glycosyl hydrolases (GH) family 49 and also testified the presence of O-glycosylation at N-terminal end.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Zohra RR,Aman A,Ansari A,Haider MS,Qader SAdoi
10.1016/j.ijbiomac.2015.04.007subject
Has Abstractpub_date
2015-07-01 00:00:00pages
243-8eissn
0141-8130issn
1879-0003pii
S0141-8130(15)00242-1journal_volume
78pub_type
杂志文章abstract::Collagen, a unique connective tissue protein finds extensive application as biocompatible biomaterial in wound healing, as drug carriers, cosmetics, etc. A work has been undertaken to study the stabilization of type I collagen using the plant polyphenol catechin. Catechin treated collagen fibres showed a shrinkage tem...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2005.08.005
更新日期:2005-10-30 00:00:00
abstract::Previous investigations on the role of the polymerase in the synthesis of poly-3-hydroxybutyrate (PHB) are reviewed, and the results from earlier in vitro studies on the activity and selectivity of the polymerase of Alcaligenes eutrophus are discussed. In the present study the effect of glycerol on stabilizing the pol...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/s0141-8130(99)00015-x
更新日期:1999-06-01 00:00:00
abstract::In this study, hetero-exopolysaccharide of Lactobacillus gasseri FR4 isolated from native chicken was produced and purified. The molecular weight of LgEPS was found to be 1.86×105Da. The gas chromatographic analysis revealed that the LgEPS was majorly composed of glucose (65.31%), mannose (16.51%), galactose (8.45%), ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.11.062
更新日期:2018-04-01 00:00:00
abstract::Chitosan (Chi) and gelatine (Gel) polymer blends with ZnO nanoparticles loaded on silica were synthesized through a simple solution method under ultrasonic irradiation at different concentration ratios (5:3, 1:3 and 3:5). The viscosity characteristics of the nanostructure were investigated by using ultrasound irradiat...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.06.231
更新日期:2019-09-15 00:00:00
abstract::Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method. The biomimetic strategy is inspired by natural mineralization processes, where the synthesized m...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.12.083
更新日期:2019-03-15 00:00:00
abstract::Concanavalin A (Con A) binding fraction of human seminal plasma is vital as it shows decapacitating activity and contains proteins which have critical roles in fertility related processes. Con A binding proteins were isolated by lectin affinity chromatography. These proteins form high molecular weight aggregates at ne...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2012.11.019
更新日期:2013-02-01 00:00:00
abstract::This study investigated the effect of Lactobacillus fermentum fermentation treatment on the gastrointestinal digestion and fermentation in vitro of polysaccharides from longan pulp. Polysaccharide isolated from unfermented and fermented longan pulp named LP and LP-F, respectively. The molecular weight of LP-F declined...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.01.059
更新日期:2020-03-15 00:00:00
abstract::Accommodation in the mammalian lens requires flexure of lens fibres and some redistribution of their contents involving limited viscous flow. Shear-dependent viscosity of bovine alpha-crystallin solutions was determined with the Contraves Low-Shear Rheometer between 4.4 and 347 mg ml(-1), and at 15.5, 25, 30, and 37 d...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/s0141-8130(02)00018-1
更新日期:2002-06-18 00:00:00
abstract::Gelatinization and retrogradation characteristics of starches from tigernut (Cyperus esculentus) tuber before and after various oil extraction processes were studied in this investigation. The results indicated that starches isolated from tigernut tuber after the various oil extraction processes varied significantly i...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.03.252
更新日期:2020-08-01 00:00:00
abstract::Biocompatible and biodegradable gelatin is a good candidate bioink for use in 3D bioprinting technologies, but viscous gelatin solution has a low printability. In order to improve the poor printability of gelatin, we optimized the rheological properties of gelatin solution. 3D gelatin scaffolds were then cross-linked ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.05.207
更新日期:2019-08-15 00:00:00
abstract::β-N-Acetylhexosaminidases (CAZy GH20, EC 3.2.1.52) are exo-glycosidases specific for cleaving N-acetylglucosamine and N-acetylgalactosamine moieties of various substrates. The β-N-acetylhexosaminidase from the filamentous fungus Talaromyces flavus (TfHex), a model enzyme in this study, has a broad substrate flexibilit...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.05.273
更新日期:2020-10-15 00:00:00
abstract::Non-mammalian collagens have attracted increasing attention for industrial and biomedical use. We have therefore evaluated extraction conditions and the biochemical properties of collagens from aquacultured sturgeon. Pepsin-soluble type I and type II collagen were respectively extracted from the skin and notochord of ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.03.091
更新日期:2019-06-15 00:00:00
abstract::Histone H1 is a basic chromosomal protein which links adjacent nucleosomes in chromatin structure. Valproic acid (VPA), a histone deacetylase inhibitor, is widely used as an antiepileptic drug for the treatment of various cancers. In this study the interaction between VPA and histone H1, chromatin and DNA in solution ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.02.098
更新日期:2017-06-01 00:00:00
abstract::Silk sericin (SS) possessing moisture-retaining property was reacted with activated poly(ethylene glycol) (PEG) to obtain self-assembled SS nanoparticles. The aliphatic and aromatic hydroxyl groups of serine and tyrosine residues as the reaction sites in SS were clarified by amino acid analysis and 1H NMR spectroscopy...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/s0141-8130(03)00023-0
更新日期:2003-03-01 00:00:00
abstract::Polysaccharides are an abundant resource in coffee beans and have proved to show numerous bioactivities. Despite their abundance, their activities are not always satisfactory mostly due to their structure and large molecular size. Molecular modifications of native polysaccharides can overcome this problem. In this stu...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.03.034
更新日期:2017-06-01 00:00:00
abstract::We have earlier in silico designed the 3-dimensional structure of a protein enriched with branched chain amino acids (BCAA, 56.4%), having only α-helical coiled-coil structure. Here, homology modeling was used to improve the in silico designed protein model. The secondary and tertiary structures of improved protein mo...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.10.133
更新日期:2020-03-01 00:00:00
abstract::Starch-based polyelectrolyte that contained azo and carboxyl groups was prepared and applied to performed layer-by-layer (LbL) assembly with amino starch. The structure of the starch-based polyelectrolyte was characterized with UV-visible spectroscopy and 1HNMR. UV-visible spectroscopy was also utilized to confirm the...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.03.106
更新日期:2019-06-15 00:00:00
abstract::Breast cancer (BC) is among the leading causes of mortality from cancer in women. Many of the available anticancer drugs have various side effects. Therefore, researchers are seeking novel anticancer agents particularly from natural compounds and in this regard, snake venom is still one of the main sources of drug dis...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.06.130
更新日期:2020-11-01 00:00:00
abstract::Forensic Science Laboratories usually receive numerous cases of suicidal, accidental, and homicidal poisoning most often involving organophosphorus (OP) pesticides. The toxicity of the OP pesticides arises due to their ability to inhibit the activity of acetylcholinesterase (AChE), a cholinergic enzyme that is essenti...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.215
更新日期:2020-12-01 00:00:00
abstract::Bacterium Bacillus sp. SS105, isolated from Free Air CO2 Enriched (FACE) soil was previously screened for carbonic anhydrase activity and CO2 sequestration. In this study, strain was selected to amplify carbonic anhydrase encoding genes. The CA genes from Bacillus sp. SS105 were found to be homologous with beta‑carbon...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.03.082
更新日期:2019-06-15 00:00:00
abstract::Seeds from avocado (Persea americana Miller) fruit are a waste byproduct of fruit processing. Starch from avocado seed is a potential alternative starch source. Two different extraction solvents were used to isolate starch from avocado seeds, functional and rheological characteristics measured for these starches, and ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.01.052
更新日期:2016-05-01 00:00:00
abstract::In this study, lipase (LIP) was isolated from Aspergillus crevinus, statistically optimized and purified via ammonium sulfate fractionation (ASF), and Sephadex G-100 gel permeation chromatography. LIP was 2.26-folds purified with a specific activity of 223.60 U/mg. The molecular mass was estimated to be 60 kDa on sodi...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.07.192
更新日期:2018-11-01 00:00:00
abstract::Here, we investigated the effect of preparation temperature and alginate-coating on L929 fibroblast behavior on lyophilized microporous PLLA/PLGA (95:5, w/w) scaffolds. The lower freezing temperature used during lyophilization (-80 °C) resulted in smaller pores (around 50 μm) and higher compressive modulus (1500 kPa) ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.11.169
更新日期:2019-03-01 00:00:00
abstract::Sodium houttuyfonate analogs (SHAs), CH(3)-(CH(2))(n)-CO-CH(2)-CH(OH)SO(3)Na, (n=6-14) were synthesized and their molecular interactions with renin and angiotensin I converting enzyme (ACE) studied using fluorescence quenching techniques. Unlike renin, inhibition of ACE activity was not directly proportional to the al...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2007.03.004
更新日期:2007-08-01 00:00:00
abstract::An efficient method was developed to prepare oxidized potato starch under the pasting point by Electro-Fenton system, in which a complex of sodium citrate and Fe2+ (SC-Fe2+) was used as catalyst, and H2O2 was used as oxidant. In the preparation process the oxidation degree of starch was controlled by the molar ratios ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.10.012
更新日期:2019-01-01 00:00:00
abstract::Bifunctional biotinylated thiosemicarbazone dextran-coated iron oxide Nanoparticles (NPs) were fabricated. Aldehyde groups of the oxidized dextran-coating layer were utilized to conjugate biotin as a tumor-targeting agent and thiosemicarbazide as a cation chelator on the surface of NPs. The final product was character...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.01.208
更新日期:2020-04-01 00:00:00
abstract::A novel glucose-sensitive drug delivery system with controlled release of metronidazole was synthesized by using biocompatible photo-crosslinked chitosan hydrogel film. Specifically, methacrylic anhydride, as photosensitive substance, was firstly grafted on chitosan molecular chains and then UV irradiation was applied...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.09.202
更新日期:2019-02-01 00:00:00
abstract::Arginine kinase (AK) plays a key role in ATP buffering systems of tissues and nerves that display high and variable rates of ATP turnover and is widely distributed in invertebrate animals. The enzyme is also found in unicellular organisms, protists and bacteria, but its occurrence is intermittent among species. The AK...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2013.02.023
更新日期:2013-06-01 00:00:00
abstract::Due to the incongruence of morphology-based hypotheses and scarcity of molecular data, validity of the order Tetraonchidea remains contentious. The only complete mitogenome currently available for the entire order is that of Paratetraonchoides inermis (Tetraonchoididae). To study the phylogeny of Tetraonchidea from mi...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.09.150
更新日期:2020-01-15 00:00:00
abstract::Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and Sephadex G-100 size-exclusion chromatography in sequence. Three main fractions MP-1, MP-3 and MP-4 were obtained during the purification steps. The recovery rates bas...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.12.007
更新日期:2017-03-01 00:00:00