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Preparation of self-assembled silk sericin nanoparticles.

Abstract：

:Silk sericin (SS) possessing moisture-retaining property was reacted with activated poly(ethylene glycol) (PEG) to obtain self-assembled SS nanoparticles. The aliphatic and aromatic hydroxyl groups of serine and tyrosine residues as the reaction sites in SS were clarified by amino acid analysis and 1H NMR spectroscopy, respectively. From IR and circular dichroism (CD) measurements, introduction of PEG into SS induced the conformational change from random coil to beta-sheet. DSC thermogram of sericin-PEG conjugate suggests that mutual miscibility between PEG and SS chains was poor. Nanoparticles of sericin-PEG conjugate with sizes measured by dynamic light scattering ranging about 200-400 nm in diameter, were prepared by the diafiltration method. Shape of sericin-PEG conjugate nanoparticles observed by scanning and transmission electron microscopes was spherical. The results suggest that sericin-PEG conjugates are self-associated to form spherical nanoparticles through hydrophobic interaction.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Cho KY,Moon JY,Lee YW,Lee KG,Yeo JH,Kweon HY,Kim KH,Cho CS

doi

10.1016/s0141-8130(03)00023-0

keywords:

subject

Has Abstract

pub_date

2003-03-01 00:00:00

pages

36-42

issue

1-2

eissn

0141-8130

issn

1879-0003

pii

S0141813003000230

journal_volume

32

pub_type

杂志文章

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