当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Comparative proteomics analysis of whitetop (Lepidium draba L.) seedlings in response to exogenous glucose.

Comparative proteomics analysis of whitetop (Lepidium draba L.) seedlings in response to exogenous glucose.

Abstract：

:In this research, a comparative proteomics approach was conducted to understand the physiological processes behind the sulforaphane formation in whitetop seedlings in response to exogenous glucose. Initially, 5-day-old whitetop seedlings were elicited by different concentrations (0, 166, 250, 277, 360 mM) of glucose for 72 h. According to the results, sulforaphane formation was influenced in a dose-dependent manner by glucose, and was maximized with the concentrations of 166 and 250 mM. Consequently, 2-dimensional gel electrophoresis was performed on the 166 mM glucose-elicited seedlings and it was shown that 25 protein spots were differentially expressed between glucose-elicited seedlings and control. Two hypothetical (were down-regulated) and 9 unique proteins (44% and 56% up- and down-regulated, respectively) were identified based on the Mass spectrometry analysis. According to the functional classification of the unique proteins, photosynthetic, chaperone, energy metabolism, signaling and sorting related proteins are marked in response to the glucose elicitation. This is the first report to successfully identify the Abscisic acid receptor PYR1-like and sorting nexin 1 isoform X1 by proteomics technique. In addition, the role of the sorting nexin 1 isoform X1 in the glucose-elicited whitetop seedling is reported for the first time.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Rezaee F,Lahouti M,Maleki M,Ganjeali A

doi

10.1016/j.ijbiomac.2018.09.016

subject

Has Abstract

pub_date

2018-12-01 00:00:00

pages

2458-2465

issue

Pt B

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(18)33951-5

journal_volume

120

pub_type

杂志文章

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