Abstract:
:To explore new antioxidant resource from food, a water-soluble polysaccharide (ALP1) was extracted and purified from the roots of Arctium lappa L. (A. lappa L.) through hot water extraction followed by ethanol precipitation, ion-exchange chromatography and gel filtration. The antioxidant activity of ALP1 was then evaluated in vitro and in vivo. ALP1 was characterized as a fructan composed of fructose and glucose in the ratio of 13.0:1.0, with an average molecular weight of 4600 Da. The linkages in ALP1 were →1)-Fruf-(2→, Fruf-(2→ and Glcp-(1→. In vitro antioxidant assays demonstrated that ALP1 possessed moderate ABTS(+) scavenging activity, strong hydroxyl radical scavenging activity and strong ferrous ion chelating activity. In in vivo antioxidant assays, ALP1 administration significantly enhanced antioxidant enzyme activities and total antioxidant capacity, as well as decreased the levels of malondialdehyde (MDA) in both the serum and liver of aging mice. These results suggest that ALP1 has potential as a novel natural antioxidant in food industry and pharmaceuticals.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Liu W,Wang J,Zhang Z,Xu J,Xie Z,Slavin M,Gao Xdoi
10.1016/j.ijbiomac.2014.01.062subject
Has Abstractpub_date
2014-04-01 00:00:00pages
446-53eissn
0141-8130issn
1879-0003pii
S0141-8130(14)00072-5journal_volume
65pub_type
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