Abstract:
:Lignin-carbohydrate complexes, i.e. LCC-48 and LCC-72 were isolated with vibrational ball milling for 48 h and 72 h, respectively. Composite tubular carriers were prepared by casting film formation and crimping with the LCCs and cellulose nanofibrils (CNFs) as raw materials. The structures and chemical properties of different milling time LCCs were analyzed. The carriers were used to culture human coronary artery endothelial cells (HCAEC), and the activities of these cells were examined in vitro. The FT-IR and chemical composition results showed that the biocarriers were composed of lignin moieties and polysaccharides. The SEM and inverted microscope studies revealed that a large number of cells adhered to the porous carriers. HCAEC grown on the LCC-72/CNF carriers outperformed the LCC-48/CNF and control groups in every observed category, including cell proliferation rate and metabolic activity. In summary, tubular carriers prepared from LCC/CNF composite had high biocompatibility and have potential applications in heart tissue engineering.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Zhao HK,Wei XY,Xie YM,Feng QHdoi
10.1016/j.ijbiomac.2019.07.062subject
Has Abstractpub_date
2019-09-15 00:00:00pages
1161-1168eissn
0141-8130issn
1879-0003pii
S0141-8130(19)34024-3journal_volume
137pub_type
杂志文章abstract::Organic solvents tend to strip water from protein and thereby disrupt non-covalent forces and decrease enzyme activity and stability. In the present study, we have replaced the surface charge residues in Salinivibrio zinc-metalloprotease (SVP) with hydrophobic ones (E12V, D22I, D24A and D310I) in order to study the ef...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.07.093
更新日期:2018-12-01 00:00:00
abstract::A novel composite hydrogel was prepared as a dual drug delivery carrier. Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) microparticles were prepared to encapsulate simultaneously ketoprofen and mupirocin, as hydrophobic drug models. These microparticles were embedded in a physically crosslinked hydrogel of κ-carragee...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.12.193
更新日期:2020-03-01 00:00:00
abstract::In the present study, high-methoxyl pectin (HMP) was extracted from Hylocereus polyrhizus peel's using physico-chemical process. In addition, the hypolipidemic activity of HMP was investigated at different concentration and time corresponding to its adsorption ability. FTIR and contact angle analysis were used to dete...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.03.143
更新日期:2019-08-01 00:00:00
abstract::Structural roles of loop regions are frequently overlooked in proteins. Nevertheless, they may be key players in the definition of protein topology and in the self-assembly processes occurring through domain swapping. We here investigate the effects on structure and stability of replacing the loop connecting the last ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.04.164
更新日期:2019-07-15 00:00:00
abstract::This paper characterizes properties of biocomposite sonicated during gelatinization. The biocomposite consisted of tapioca starch based plastic reinforced by 10% volume fraction of water hyacinth fiber (WHF). During gelatinization, the biocomposite was poured into a rectangular glass mold then vibrated in an ultrasoni...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.11.165
更新日期:2018-03-01 00:00:00
abstract::In present study, HEP was successfully encapsulated into the Poly (lactic-coglycolicacid) (PLGA) to constitute the HEP-PLGA. The effects of three independent factors (the proper range of ratio of organic phase (o) to internal water phase (w1) (X1), ratio of external water phase (w2) to the primary emulsion (PE) (X2), ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.06.169
更新日期:2018-10-15 00:00:00
abstract::Pullulan (PU) is an exo-polysaccharide used in the food and pharmaceutical industries. However, the use of PU in different industries is limited due to its highly hydrophilic nature and consequently weakness in surface properties which can be remedied by its chemical modification with octenyl succinic anhydride (OSA)....
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.158
更新日期:2020-12-01 00:00:00
abstract::Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm characterized by the presence of the Bcr-Abl tyrosine kinase protein, which confers resistance to apoptosis in leukemic cells. Tyrosine kinase inhibitors (TKIs) are effectively used to treat CML; however, CML patients in the advanced (CML-AP) and chronic ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.01.069
更新日期:2016-05-01 00:00:00
abstract::A novel magnetic chitosan adsorbent (CDF-CS) was synthesized by inserting magnetic particles into the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution. The characterization and adsorption mechanism of CDF-CS were studied by SEM-EDS, V...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.07.022
更新日期:2019-09-15 00:00:00
abstract::Antioxidant effects of lentinan on LPS induced inflammatory response in intestine of juvenile taimen were evaluated, and its prebiotic-like efficacy on intestinal microbiota was also investigated. The results showed that LPS decreased the activities of antioxidant enzymes and increased the expression levels of inflamm...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.09.121
更新日期:2019-01-01 00:00:00
abstract::The present study focused on preparation screening, production optimization and characterization of exopolysaccharides (EPS) produced by Lactobacillus sanfranciscensis isolated from a Chinese traditional sourdough sample. In addition, in vitro antioxidant activity of pure EPS was determined. A total of 51 strains of L...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.08.077
更新日期:2019-10-15 00:00:00
abstract::The purpose work is devoted to design of a simple, one-pot and green approach for the synthesis of molecularly imprinted polymer to construct a selective sorbent for pipette-tip solid phase extraction of Rhodamine B from chili powder samples and its subsequence separation and quantification by high performance liquid ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.07.196
更新日期:2019-10-15 00:00:00
abstract::Twelve therapeutic mAbs, comprising 10 IgG1s and 2 IgG4s, were analyzed by a peptide mapping technique to detect and quantify C-terminal modifications. C-terminal amidated structures were found in 8 out of the 12 mAbs. An in vitro study using a commercially available peptidylglycine alpha-amidating monooxygenase (PAM)...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2012.09.016
更新日期:2013-01-01 00:00:00
abstract::Coriolus versicolor is an edible medicinal mushroom in China. Two polysaccharides, named as CVPn and CVPa were separated from the dried fruiting bodies of Coriolus versicolor by water extraction and ethanol precipitation. Their chemical structures were well elucidated with overall consideration of monosaccharide compo...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.11.018
更新日期:2021-01-01 00:00:00
abstract::Biopolymers are widely used for the removal of chromium from aqueous medium but it possesses limitations like poor sorption capacity and low stability. To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chrom...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.10.010
更新日期:2016-02-01 00:00:00
abstract::Conjugation of various arginine-rich peptide sequences to vectors based on 10 kDa polyethylenimine (PEI) and its hydrophobic derivative (hexanoate-PEI) was investigated as a strategy for improving pDNA and siRNA transfection activities. Six different arginine-histidine (RH) sequences and two arginine-serine (RS) seque...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2013.05.001
更新日期:2013-09-01 00:00:00
abstract::This review article summarises the possible mechanisms of the protein-ligand interaction, folding, misfolding, aggregation and inhibition of protein aggregates. Under certain stressed condition the folding process deviates from its path and results into misfolding and aggregation of proteins. So aggregates have to be ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
doi:10.1016/j.ijbiomac.2017.07.185
更新日期:2018-01-01 00:00:00
abstract::Alginate (ALg)-human-like collagen (HLC) microspheres were prepared by the technology of electrostatic droplet generation in order to develop a biocompatible vehicle for probiotic bacteria. Microparticles were spherical with mean particle size of 400μm. The encapsulation efficiency (EE) of ALg-HLC microspheres could r...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2011.08.018
更新日期:2011-12-01 00:00:00
abstract::Lectins are a group of widely distributed and structurally heterogeneous proteins of nonimmune origin. These proteins have the ability to interact with glycans present on cell surfaces and elicit diverse biological activities. Machaerium acutifolium lectin (MaL) is an N-acetyl-D-glucosamine-binding lectin that exhibit...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.06.236
更新日期:2020-11-15 00:00:00
abstract::It is well accepted that porphyrins related to heme, are effectively able to act as pharmaceutical. For instance, they were found to interfere with the aggregation of beta-amyloid peptide. In present study, the influence of heme concentration on the amyloid-type aggregation of ovalbumin was investigated. We provided e...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2008.10.011
更新日期:2009-01-01 00:00:00
abstract::In this study the extraction of hot water soluble polysaccharides (HWSPs) from Cymbopogon citratus using hot water decoction was discussed. Response surface methodology (RSM) based on a three level, three variable central composite rotatable design (CCRD), was employed to obtain best possible combination of extraction...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2014.01.033
更新日期:2014-04-01 00:00:00
abstract::Agrobacterium tumefaciens uronate dehydrogenase (AtuUdh) belongs to the short-chain dehydrogenase superfamily, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. It is apparently required for the production of D-glucaric acid. AtuUdh-catalyzed reaction is reversible with dual substra...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.08.194
更新日期:2019-11-01 00:00:00
abstract::Abiotic stresses enhance cellular reactive oxygen species (ROS) level which results in toxic methylglyoxal (MG) production. Glyoxalases catalyze the conversion of toxic MG into non-toxic lactic acid whose properties and function are still unknown in cyanobacteria. This is the first attempt to characterize All0580 from...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.11.172
更新日期:2019-03-01 00:00:00
abstract::When not incorporated into the casein micelle, isolated κ-casein spontaneously forms amyloid fibrils under physiological conditions, and is a convenient model for researching generic aspects of fibril formation. Ginsenosides have recently attracted much research interest because of the effects on aging diseases, which...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.04.046
更新日期:2015-08-01 00:00:00
abstract::Toward the development of biocomposites for packaging applications, the possibility of using kenaf cellulose (KC) was investigated in the production of low-density polyethylene (LDPE)/KC/polyethylene glycol (PEG) biocomposites. First, cellulose was extracted from the cell walls of kenaf-bast fibers. Then, different we...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2010.04.004
更新日期:2010-08-01 00:00:00
abstract::We report here the self-assembled structures of polyelectrolyte complexes (PECs) of polyanionic sodium alginate with the polycationic chitosan at room temperature. The PECs prepared at different pH values exhibited two distinct morphologies. The chitosan-alginate PECs self-assembled into the fibrous structure in a low...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.03.075
更新日期:2018-07-15 00:00:00
abstract::Golgi Reassembly and Stacking Proteins (GRASPs) were firstly described as crucial elements in determining the structure of the Golgi complex. However, data have been accumulating over the years showing GRASPs can participate in various cell processes beyond the Golgi maintenance, including cell adhesion and migration,...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
doi:10.1016/j.ijbiomac.2020.08.203
更新日期:2020-12-01 00:00:00
abstract::A chitosan-glutaraldehyde crosslinked uricase was immobilized onto Prussian blue nanoparticles (PBNPs) absorbed onto carboxylated multiwalled carbon nanotube (c-MWCNT) and polyaniline (PANI) layer, electrochemically deposited on the surface of Au electrode. The nanohybrid-uricase electrode was characterized by scannin...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2011.10.002
更新日期:2012-01-01 00:00:00
abstract::Bacterially synthesized poly(hydroxyalkanoate)s (PHAs) suffers from low crystallization rate which is enhanced by using tailor-made oxalamide compounds as nucleators. The influence of nucleator configurations on the crystallization behaviour of the PHAs was investigated using differential scanning calorimetry (DSC), p...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.06.001
更新日期:2017-11-01 00:00:00
abstract::In the present study, Penicillium proteolyticum lectin was purified by DEAE-Sepharose chromatography followed by Sephadex G-100 gel filtration chromatography. A sequence of ion-exchange and gel filtration chromatography resulted in 52.30 fold purified lectin with a high yield of 84.21%. The purified P. proteolyticum l...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.01.103
更新日期:2019-05-01 00:00:00