The exquisite structural biophysics of the Golgi Reassembly and Stacking Proteins.


:Golgi Reassembly and Stacking Proteins (GRASPs) were firstly described as crucial elements in determining the structure of the Golgi complex. However, data have been accumulating over the years showing GRASPs can participate in various cell processes beyond the Golgi maintenance, including cell adhesion and migration, autophagy and unconventional secretion of proteins. A comprehensive understanding of the GRASP functions requires deep mechanistic knowledge of its structure and dynamics, especially because of the unique structural plasticity observed for many members of this family coupled with their high promiscuity in mediating protein-protein interactions. Here, we critically review data regarding the structural biophysics of GRASPs in the quest for understanding the structural determinants of different functionalities. We dissect GRASP structure starting with the full-length protein down to its separate domains (PDZ1, PDZ2 and SPR) and outline some structural features common to all members of the GRASP family (such as the presence of many intrinsically disordered regions). Although the impact of those exquisite properties in vivo will still require further studies, it is possible, from our review, to pinpoint factors that must be considered in future interpretation of data regarding GRASP functions, thus bringing somewhat new perspectives to the field.


Int J Biol Macromol


Mendes LFS,Fontana NA,Reddy ST,Uversky VN,Costa-Filho AJ




Has Abstract


2020-12-01 00:00:00












  • The effect of aluminum oxide on red blood cell integrity and hemoglobin structure at nanoscale.

    abstract::Herein, we explored the interaction of Al2O3 NPs with RBCs and Hb to determine the effect of Al2O3 NPs on hemolytic activity and Hb denaturation. The percentage of hemolysis of extracts and direct contact assays triggered by Al2O3 NPs was calculated by determining supernatant Hb concentration at 540 nm. Far-UV CD and ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Kahbasi S,Samadbin M,Attar F,Heshmati M,Danaei D,Rasti B,Salihi A,Nanakali NMQ,Aziz FM,Akhtari K,Hasan A,Falahati M

    更新日期:2019-10-01 00:00:00

  • Biological interaction of newly synthesized astaxanthin-s-allyl cysteine biconjugate with Saccharomyces cerevisiae and mammalian α-glucosidase: In vitro kinetics and in silico docking analysis.

    abstract::In humans, alpha-glucosidase activity is present in sucrase-isomaltase (SI) and maltase-glucoamylase (MGAM). α-glucosidase is involved in the hydrolyses of disaccharide into monosaccharides and results in hyperglycemia. Subsequently chronic hyperglycemia induces oxidative stress and ultimately leads to the secondary c...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Sakayanathan P,Loganathan C,Iruthayaraj A,Periyasamy P,Poomani K,Periasamy V,Thayumanavan P

    更新日期:2018-10-15 00:00:00

  • Tailoring the surface properties and flexibility of starch-based films using oil and waxes recovered from potato chips byproducts.

    abstract::Agrofood byproducts may be exploited as a source of biomolecules suitable for developing bioplastic materials. In this work, the feasibility of using starch, oil, and waxes recovered from potato chips byproducts for films production was studied. The recovered potato starch-rich fraction (RPS) contained an amylopectin/...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Gonçalves I,Lopes J,Barra A,Hernández D,Nunes C,Kapusniak K,Kapusniak J,Evtyugin DV,Lopes da Silva JA,Ferreira P,Coimbra MA

    更新日期:2020-11-15 00:00:00

  • Temperature effects on alpha-crystallin structure probed by 6-bromomethyl-2-(2-furanyl)-3-hydroxychromone, an environmentally sensitive two-wavelength fluorescent dye covalently attached to the single Cys residue.

    abstract::The single Cys residue in the C-terminal domain of bovine eye lens alpha-crystallin was covalently labelled with 6-bromomethyl-2-(2-furanyl)-3-hydroxychromone. This novel SH-reactive two-band ratiometric fluorescent dye is characterized by excited state intramolecular proton transfer reaction yielding two highly emiss...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Avilov SV,Bode C,Tolgyesi FG,Klymchenko AS,Fidy J,Demchenko AP

    更新日期:2005-09-28 00:00:00

  • Chelator-mediated biomimetic degradation of cellulose and chitin.

    abstract::Non-enzymatic degradation of wood via a chelator-mediated Fenton (CMF) system is the primary method for initial attack in brown rot fungal decomposition of wood, the most common type of fungal degradation of terrestrial carbon biomass on the planet. In this study, the degradation of thin films of cellulose and chitin ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Liu J,Zhu Y,Wang C,Goodell B,Esker AR

    更新日期:2020-06-15 00:00:00

  • Effect of o-acyl substituents on the functional behaviour of Rhizobium meliloti succinoglycan.

    abstract::The effects of selective removal of acetyl or succinyl substituents on the functionality of succinoglycan polysaccharide have been studied by comparing the behaviour of the polysaccharides isolated from native Rhizobium meliloti strain Rm1021, and genetically modified R. meliloti species. Removal of the succinyl group...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Ridout MJ,Brownsey GJ,York GM,Walker GC,Morris VJ

    更新日期:1997-02-01 00:00:00

  • The effects of daunomycin antibiotic on histone H(1): thermal denaturation and fluorescence spectroscopy studies.

    abstract::Using thermal denaturation and fluorescence spectroscopy, we have investigated the interaction of antitumor antibiotic, daunomycin, with calf thymus histone H(1) under several ionic strengths. The results show that daunomycin binds to histone H(1) and increases its melting temperature. Increasing ionic strength elevat...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Zargar SJ,Rabbani A

    更新日期:2000-10-10 00:00:00

  • The protective effect of polysaccharide extracted from Portulaca oleracea L. against Pb-induced learning and memory impairments in rats.

    abstract::This paper studied the extraction of polysaccharide from Portulaca oleracea L. (POP) by hot water extraction and ethanol precipitation. Structural properties of the extracted polymers were determined. POP was composed of rhamnose, arabinose and galactose in ratios of 1: 2.34: 3.07 with a molecular weight of 1.55 × 107...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Tao H,Ye DL,Wu YL,Han MM,Xue JS,Liu ZH,Chen XT,Wang HL

    更新日期:2018-11-01 00:00:00

  • Pickering emulsion process assisted construction of regenerated chitin reinforced poly (lactic acid) blends.

    abstract::Regenerated chitin (RCh)/poly (lactic acid) (PLA) blends with enhanced properties were successfully prepared via the Pickering emulsion approach. An oil-in-water Pickering emulsion of PLA/dichloromethane (DCM) stabilized with RCh via an ultrasonic homogenizer was formed. A uniformly dispersed blends were obtained upon...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Munyazesa FX,Zhang Y,Wang B,Wang C,Feng X,Mao Z,Chen Y,Sui X

    更新日期:2019-11-01 00:00:00

  • Cellulose porosity improves its dissolution by facilitating solvent diffusion.

    abstract::This paper reports on the effect of improved porous characteristics of cellulose on its solubility in DMAc/LiCl. The use of freeze-drying (FD) treatment led to higher surface area and void fraction of volume for high-molecular-weight (HMW) cotton cellulose (DP~5000). Improvement in porous characteristics of cellulose ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Hu Y,Acharya S,Abidi N

    更新日期:2019-02-15 00:00:00

  • Rifampicin as a novel tyrosinase inhibitor: Inhibitory activity and mechanism.

    abstract::In this study, the inhibitory effect and mechanism of rifampicin on the activity of tyrosinase were investigated for developing a novel tyrosinase inhibitor. It was found to have a significant inhibition on the activity of tyrosinase (IC50=90±0.6μM). From the kinetics analysis, it was proved to be a reversible and non...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Chai WM,Lin MZ,Song FJ,Wang YX,Xu KL,Huang JX,Fu JP,Peng YY

    更新日期:2017-09-01 00:00:00

  • Catalytic phenol removal using entrapped cross-linked laccase aggregates.

    abstract::Laccase was immobilized using a combinatorial strategy of cross-linking and entrapping in mesoporous silica to prepare entrapped enzyme species including simply adsorbed, entrapped cross-linked enzyme (E-CLE) and entrapped cross-linked enzyme aggregate (E-CLEA) to explore their potential in phenol removal. Parameters ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Fathali Z,Rezaei S,Faramarzi MA,Habibi-Rezaei M

    更新日期:2019-02-01 00:00:00

  • Activated hyaluronic acid/collagen composite hydrogel with tunable physical properties and improved biological properties.

    abstract::Self-crosslinkable and injectable hydrogels were fabricated with collagen type I (Col I) and N-hydroxy sulfosuccinimide activated hyaluronic acid (HA-sNHS) at physiological conditions without any initiators or crosslinkers. The physical properties of hydrogels, such as gelation time, swelling property, degradation pro...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Gao Y,Liu Q,Kong W,Wang J,He L,Guo L,Lin H,Fan H,Fan Y,Zhang X

    更新日期:2020-12-01 00:00:00

  • Injectable self-healing carboxymethyl chitosan-zinc supramolecular hydrogels and their antibacterial activity.

    abstract::Injectable and self-healing hydrogels have found numerous applications in drug delivery, tissue engineering and 3D cell culture. Herein, we report an injectable self-healing carboxymethyl chitosan (CMCh) supramolecular hydrogels cross-linked by zinc ions (Zn2+). Supramolecular hydrogels were obtained by simple additio...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Wahid F,Zhou YN,Wang HS,Wan T,Zhong C,Chu LQ

    更新日期:2018-07-15 00:00:00

  • In vivo antioxidant and anti-inflammatory effects of soluble dietary fiber Konjac glucomannan in type-2 diabetic rats.

    abstract::Although the anti-diabetic properties of Konjac glucomannan (KGM) have been reported previously; however, the molecular pathways of its anti-diabetic properties are not clear. The present study hypothesized that KGM could mitigate oxidative stress and inflammation. Three doses of KGM (40, 80, 120 mg/kg b.w.) decreased...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Zhao Y,Jayachandran M,Xu B

    更新日期:2020-09-15 00:00:00

  • Papain-like cysteine proteinase zone (PCP-zone) and PCP structural catalytic core (PCP-SCC) of enzymes with cysteine proteinase fold.

    abstract::There are several families of cysteine proteinases with different folds - for example the (chymo)trypsin fold family and papain-like fold family - but in both families the hydrolase activity of cysteine proteinases requires a cysteine residue as the catalytic nucleophile. In this work, we have analyzed the topology of...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Denessiouk K,Uversky VN,Permyakov SE,Permyakov EA,Johnson MS,Denesyuk AI

    更新日期:2020-12-15 00:00:00

  • High yield production of levan-type fructans by Gluconobacter japonicus LMG 1417.

    abstract::Levan, a β-2,6-glycosidic linked fructan, is a promising alternative for the inulin dominated fructan market. Although levan is already used in some cosmetic products, the commercial availability of the fructan is still limited. Here we show that Gluconobacter japonicus LMG 1417 is a potent levan-forming organism and ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Hövels M,Kosciow K,Kniewel J,Jakob F,Deppenmeier U

    更新日期:2020-12-01 00:00:00

  • It takes two to tango - The case of thebaine 6-O-demethylase.

    abstract::Thebaine 6-O-demethylase (T6ODM) is an Fe(II)/2-oxoglutarate-dependent dioxygenase catalysing two oxidative O-demethylation reactions in morphine biosynthesis. Its crystal structure revealed a large active site pocket which is at least two times larger than necessary to accommodate a substrate (thebaine or oripavine) ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Kachhap S,Wojdyla Z,Komorek P,Kluza A,Kurpiewska K,Jachimska B,Borowski T

    更新日期:2020-11-15 00:00:00

  • A thermophilic and thermostable xylanase from Caldicoprobacter algeriensis: Recombinant expression, characterization and application in paper biobleaching.

    abstract::A novel xylanase gene xynBCA, encoding a polypeptide of 439 residues (XynBCA), was cloned from Caldicoprobacter algeriensis genome and recombinantly expressed in Escherichia coli BL21(DE3). The amino acid sequence analysis showed that XynBCA belongs to the glycoside hydrolase family 10. The purified recombinant enzyme...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Mhiri S,Bouanane-Darenfed A,Jemli S,Neifar S,Ameri R,Mezghani M,Bouacem K,Jaouadi B,Bejar S

    更新日期:2020-12-01 00:00:00

  • Purification and characterization of an antibacterial and anti-inflammatory polypeptide from Arca subcrenata.

    abstract::A polypeptide coded as PGC was isolated from Arca subcrenata muscle using ion exchange, Sephadex G-50 gel chromatography and RP-HPLC. PGC was identified to be a homogeneous compound by Native-PAGE and the purity was more than 98.9% measured by HPLC. The isoelectric point of PGC was determined to be 9.76 by IEF-PAGE. T...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Chen Y,Li C,Zhu J,Xie W,Hu X,Song L,Zi J,Yu R

    更新日期:2017-03-01 00:00:00

  • Orabase formulation with cashew gum polysaccharide decreases inflammatory and bone loss hallmarks in experimental periodontitis.

    abstract::This study aimed to investigate the chemical characteristics and the effects of an orabase gel with Cashew Gum Polysaccharide (CG-P) from Anacardium occidentale L. on alveolar bone loss and relative mRNA expression of TNF-α, IL-1β, RANK, RANKL, and OPG in the periodontal tissue of Wistar rats (Rattus norvegicus) subje...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Souza Filho MD,Medeiros JVR,Vasconcelos DFP,Silva DA,Leódido ACM,Fernandes HF,Silva FRP,França LFC,Lenardo D,Pinto GR

    更新日期:2018-02-01 00:00:00

  • Optimization to development of chitosan decorated polycaprolactone nanoparticles for improved ocular delivery of dorzolamide: In vitro, ex vivo and toxicity assessments.

    abstract::The present research work was designed to develop dorzolamide-loaded chitosan-coated polycaprolactone nanoparticles (DRZ-CS-PCL-NPs) for improved ocular delivery. The nanoparticles were prepared by single-step emulsification technique and optimized using the three-factor three-level Box-Behnken design. The optimized D...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Shahab MS,Rizwanullah M,Alshehri S,Imam SS

    更新日期:2020-11-15 00:00:00

  • Cellulosic paper with high antioxidative and barrier properties obtained through incorporation of tannin into kraft pulp fibers.

    abstract::In this work, we prepared novel cellulosic paper by incorporating tannin into the kraft pulp for potential application in active food packaging. The kraft pulp fibers were firstly periodate oxidized to obtain the dialdehyde cellulosic fibers, and then reacted with varied dosages of tannin to incorporate them into the ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Ji Y,Xu Q,Jin L,Fu Y

    更新日期:2020-11-01 00:00:00

  • Spectroscopic analysis of the interaction of valproic acid with histone H1 in solution and in chromatin structure.

    abstract::Histone H1 is a basic chromosomal protein which links adjacent nucleosomes in chromatin structure. Valproic acid (VPA), a histone deacetylase inhibitor, is widely used as an antiepileptic drug for the treatment of various cancers. In this study the interaction between VPA and histone H1, chromatin and DNA in solution ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Sargolzaei J,Rabbani-Chadegani A,Mollaei H,Deezagi A

    更新日期:2017-06-01 00:00:00

  • Antifungal property of quaternized chitosan and its derivatives.

    abstract::Five water-soluble chitosan derivatives were carried out by quaternizing either iodomethane or N-(3-chloro-2-hydroxypropyl) trimethylammonium chloride (Quat188) as a quaternizing agent under basic condition. The degree of quaternization (DQ) ranged between 28±2% and 90±2%. The antifungal activity was evaluated by usin...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Sajomsang W,Gonil P,Saesoo S,Ovatlarnporn C

    更新日期:2012-01-01 00:00:00

  • Time-domain NMR relaxometry as an alternative method for analysis of chitosan-paramagnetic ion interactions in solution.

    abstract::Chitosan (CHI)-paramagnetic ion interactions in aqueous solution as affected by pH were studied using time-domain NMR (TD-NMR) relaxometry. Longitudinal (T1) and transverse (T2) relaxation times of CHI solutions with Fe3+, Cu2+, and Mn2+ were measured using a single-shot pulse sequence named CP-CWFPx-x. The results in...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Kock FV,Monaretto T,Colnago LA

    更新日期:2017-05-01 00:00:00

  • Characterization of low-cost glycolipoprotein biosurfactant produced by Lactobacillus plantarum 60 FHE isolated from cheese samples using food wastes through response surface methodology and its potential as antimicrobial, antiviral, and anticancer activi

    abstract::Considering the need of new lactic acid bacteria (LAB) for the production of novel biosurfactant (BS) molecules, the current study brings out a new insight on the exploration of cheese samples for BS producers and process optimization for industrial applications. In view of this, Lactobacillus plantarum 60FHE, Lactoba...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Sakr EAE,Ahmed HAE,Abo Saif FAA

    更新日期:2021-02-15 00:00:00

  • Antioxidant and hepatoprotective potential of endo-polysaccharides from Hericium erinaceus grown on tofu whey.

    abstract::Three fractions of the endo-polysaccharides from the mycelium of Hericium erinaceus (HEP) grown on tofu whey were obtained by the fractional precipitation with gradient concentrations of ethanol (HEP40, HEP60 and HEP80). The chemical and physical characteristics of the three crude polysaccharides were investigated by ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Zhang Z,Lv G,Pan H,Pandey A,He W,Fan L

    更新日期:2012-12-01 00:00:00

  • Mathematical modeling of gallic acid release from chitosan films with grape seed extract and carvacrol.

    abstract::Controlled release of antimicrobial and antioxidant compounds from packaging films is of utmost importance for extending the shelf-life of perishable foods. This study focused on the mathematical modeling of gallic acid release into an aqueous medium from three chitosan films, formulated with grape seed extract (GSE) ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Rubilar JF,Cruz RMS,Zuñiga RN,Khmelinskii I,Vieira MC

    更新日期:2017-11-01 00:00:00

  • Preparation and application of a polymer with pH/temperature-responsive targeting.

    abstract::Targeted drug carrier systems not only prolong the long-term circulation of drugs, but also improve their bioavailability. To obtain a pH/temperature synergistically responsive polymer carrier, temperature and pH-sensitive groups were chemically grafted onto a cassava starch backbone. Secondly, the structure of the po...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Kou Z,Dou D,Mo H,Ji J,Lan L,Lan X,Zhang J,Lan P

    更新日期:2020-12-15 00:00:00