当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Experimental and theoretical study of IBC domain from human IP3R2; molecular cloning, bacterial expression and protein purification.

Experimental and theoretical study of IBC domain from human IP3R2; molecular cloning, bacterial expression and protein purification.

Abstract：

:IP3 is a ubiquitous second messenger in eukaryotic cells that triggers Ca2+-release from intracellular stores. IP3 binds to intracellular IP3-receptor (IP3R) and induces conformational change within the ligand-binding domain which regulates Ca2+-release; hence, both IP3 and IP3R are key components of the signal transduction mechanism. Here we present cDNA cloning of IP3-binding core (IBC) domain encoding only residues 224-604 of human IP3R type 2 that binds to IP3 with high affinity. RNA extraction, RT-PCR, PCR and cloning were carried out, and then the cloned DNA was checked by sequencing. Thereafter, expression vector pET-28a harboring the correct gene was transformed into different E. coli (DE3) strains and investigated its protein expression under various conditions. Finally, the IBC expression was induced at 20 °C for 20 h into BL21 strain at LB medium with 4 mM lactose and 0.5 mM IPTG, and then confirmed by western blotting. After protein purification, structural study was recorded in absence and presence of its ligand. Far-CD and intrinsic fluorescence spectra analysis of the purified protein with and without IP3 ligand showed change in secondary and tertiary IBC structure. Moreover, bioinformatics study demonstrated that the ligand binding site residues R269, K508 and R511 are conserved.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Amroudie MN,Ataei F

doi

10.1016/j.ijbiomac.2018.09.117

subject

Has Abstract

pub_date

2019-03-01 00:00:00

pages

1321-1327

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(18)33982-5

journal_volume

124

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Characterization and application of keratinolytic paptidases from Bacillus spp.

    abstract：:Solid keratin-rich waste management is one of essential research area in nowadays. Conventional chemical and high thermal keratin waste decomposition methods are fully explored and not enough effective for future biotechnology perspectives. However, traditional keratin-rich waste decomposition methods could be replace...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.03.046

    authors： Gegeckas A,Šimkutė A,Gudiukaitė R,Čitavičius DJ

    更新日期：2018-07-01 00:00:00

  • Dendrobium officinale polysaccharides attenuate learning and memory disabilities via anti-oxidant and anti-inflammatory actions.

    abstract：:The aim of this study was to explore the therapeutic effect and underling mechanism of Dendrobium officinale polysaccharides (DOPS) on two well-established animal models of learning and memory disabilities. Model of estrogen deficiency caused learning and memory disability can be induced by ovariectomy in mice, and mi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.12.230

    authors： Liang J,Wu Y,Yuan H,Yang Y,Xiong Q,Liang C,Li Z,Li C,Zhang G,Lai X,Hu Y,Hou S

    更新日期：2019-04-01 00:00:00

  • Characterization by mass spectrometry of poly(3-hydroxyalkanoates) produced by Rhodospirillum rubrum from 3-hydroxyacids.

    abstract：:The sequence distributions of two microbial copolyesters obtained by fermentation of Rhodospirillum rubrum, grown with 3-hydroxyhexanoic or 3-hydroxyheptanoic acids, were determined by analyzing the oligomers prepared by partial pyrolysis or partial methanolysis of these copolyesters using fast atom bombardment mass s...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/s0141-8130(99)00085-9

    authors： Ballistreri A,Giuffrida M,Impallomeni G,Lenz RW,Fuller RC

    更新日期：1999-11-01 00:00:00

  • The first complete mitochondrial genome from the family Hygrophoraceae (Hygrophorus russula) by next-generation sequencing and phylogenetic implications.

    abstract：:Hygrophorus russula (Schaeff.) Kauffman is an edible ectomycorrhizal fungus that is widely distributed in the world. In this study, the mitogenome of H. russula was sequenced and assembled. The mitogenome of H. russula is composed of circular DNA molecules, with a total size of 55,769 bp. Further analysis indicated th...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.09.091

    authors： Li Q,Wang Q,Jin X,Chen Z,Xiong C,Li P,Zhao J,Huang W

    更新日期：2019-02-01 00:00:00

  • Cationic starch nanoparticles based on polyelectrolyte complexes.

    abstract：:Cationic starch nanoparticles were obtained by aqueous polyelectrolyte complex formation between cationic quaternary ammonium substituted starches and anionic sodium tripolyphosphate. The formation of nanosized starch particles of spherical shape was verified by dynamic light scattering and scanning electron microscop...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2012.01.037

    authors： Rutkaite R,Bendoraitiene J,Klimaviciute R,Zemaitaitis A

    更新日期：2012-04-01 00:00:00

  • Effects of germination followed by hot air and infrared drying on properties of naked barley flour and starch.

    abstract：:Naked barley grains were germinated at 25 °C for 12, 24, and 36 h, followed by hot air and infrared drying. Changes in structural, physicochemical, and functional properties of naked barley flour and starch after germination and drying were evaluated. Germinated and dried flour showed loose structure in starch granule...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.10.114

    authors： Su C,Saleh ASM,Zhang B,Feng D,Zhao J,Guo Y,Zhao J,Li W,Yan W

    更新日期：2020-12-15 00:00:00

  • Quantification of anti-aggregation activity of UV-irradiated α-crystallin.

    abstract：:Ultraviolet radiation is a risk factor for cataractogenesis. It is believed that enhanced rates of lens opacification and cataract formation are the results of gradual loss of chaperone-like efficiency of α-crystallin upon exposure to UV light. To characterize chaperone-like activity of α-crystallin damaged by UV irra...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.10.060

    authors： Borzova VA,Markossian KA,Muranov KO,Polyansky NB,Kleymenov SY,Kurganov BI

    更新日期：2015-02-01 00:00:00

  • Production and characterization of hydrophilic and hydrophobic sunflower protein isolate nanofibers by electrospinning method.

    abstract：:In the present study, sunflower protein isolate (SPI) was extracted and applied for production of nanofiber using polyvinyl alcohol (PVA) in different SPI:PVA volume ratios (0:100, 20:80, 40:60, 60:40, 80:20 and 100:0) under two voltages (18 and 23 kV) and two flow rates (0.5 and 0.75 ml/h). Nanofibers with average di...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.07.132

    authors： Shanesazzadeh E,Kadivar M,Fathi M

    更新日期：2018-11-01 00:00:00

  • Deciphering the interactions of fish gelatine and hyaluronic acid in aqueous solutions.

    abstract：:The interactions of fish gelatine (FG) with hyaluronic acid (HA) are studied in an aqueous environment at 25°C by turbidimetric titration, confocal scanning laser microscopy, dynamic light scattering, zeta potentiometry, spectrophotometry with methylene blue, and construction of state diagrams. FG forms soluble comple...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.04.083

    authors： Razzak MA,Kim M,Kim HJ,Park YC,Chung D

    更新日期：2017-09-01 00:00:00

  • Copolymers of xylan-derived furfuryl alcohol and natural oligomeric tung oil derivatives.

    abstract：:The utilization of vegetable oils as biological oligomers for the synthesis of macromolecular materials has considerably evolved in the last decades, opening the way for the preparation of sophisticated materials based on synthetic processes for the design of polymers with very specific applications. Tung oil (TO), ea...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.08.095

    authors： Ribeiro BO,Valério VS,Gandini A,Lacerda TM

    更新日期：2020-12-01 00:00:00

  • Statistical optimization and operational stability of Rhizomucor miehei lipase supported on magnetic chitosan/chitin nanoparticles for synthesis of pentyl valerate.

    abstract：:The chemical-catalyzed transesterification process to produce biofuels i.e. pentyl valerate (PeVa) is environmentally unfriendly, energy-intensive with tedious downstream treatment. The present work reports the use of Rhizomucor miehei lipase (RML) crosslinked onto magnetic chitosan/chitin nanoparticles (RML-CS/CH/MNP...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.04.111

    authors： Rahman INA,Attan N,Mahat NA,Jamalis J,Abdul Keyon AS,Kurniawan C,Wahab RA

    更新日期：2018-08-01 00:00:00

  • Xyloglucan-based hybrid nanocomposite with potential for biomedical applications.

    abstract：:Natural polymer-based hybrid nanocomposites have been proposed as one of the most promising tools for biomedical applications, including disease treatment and diagnosis procedures. Xyloglucan nanocapsules can simultaneously load magnetic iron oxide nanoparticles and bioactive for a specific tissue, reducing the proces...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.11.128

    authors： da Silva ACC,de Almeida RR,da Cruz Sousa AC,Martínez FNA,Denardin JC,de Morais SM,Ricardo NMPS

    更新日期：2021-01-31 00:00:00

  • Cloning and expression of in silico modeled protein enriched with branched chain amino acids in Pichia pastoris.

    abstract：:We have earlier in silico designed the 3-dimensional structure of a protein enriched with branched chain amino acids (BCAA, 56.4%), having only α-helical coiled-coil structure. Here, homology modeling was used to improve the in silico designed protein model. The secondary and tertiary structures of improved protein mo...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.10.133

    authors： L S,Vasu P

    更新日期：2020-03-01 00:00:00

  • Studies on structure and antioxidant properties of a heteroglycan isolated from wild edible mushroom Lentinus sajor-caju.

    abstract：:A water-soluble heteroglycan (PS-I) isolated from the aqueous extract of a wild edible mushroom Lentinus sajor-caju showed average molecular weight ∼1.79×105Da. The structure of the polysaccharide was determined using chemical and 1D/2D NMR experiments. Acid hydrolysis indicated the presence of d-glucose, d-galactose,...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.08.163

    authors： Pattanayak M,Maity P,Samanta S,Sen IK,Manna DK,Nandi AK,Ghosh S,Acharya K,Islam SS

    更新日期：2018-02-01 00:00:00

  • Structural and morphological evaluation of CNC reinforced PVA/Starch biodegradable films.

    abstract：:Nanocomposite films containing cellulose nanocrystals (CNC), as the reinforcing phase, and poly(vinyl alcohol) (PVA)/starch (S) blend, as the matrix, were prepared by solution casting technique. For the preparation, 5, 10, 15 wt% CNC suspension were incorporated in blends containing 90 wt% PVA/10 wt% S, 75 wt% PVA/25 ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.05.036

    authors： Popescu MC,Dogaru BI,Goanta M,Timpu D

    更新日期：2018-09-01 00:00:00

  • Topoisomerase inhibition and albumin interaction studies of acridine-thiosemicarbazone derivatives.

    abstract：:In the present study, acridine-thiosemicarbazones (ATD) derivatives were tested for their interaction properties with BSA through UV-Vis absorption and fluorescence spectroscopic studies. Both hyperchromic and hypochromic effects, as well as red or blue shifts were demonstrated after the derivatives were added to the ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.07.097

    authors： da Silva Filho FA,de Freitas Souza T,Ribeiro AG,Alves JEF,de Oliveira JF,de Lima Souza TRC,de Moura RO,do Carmo Alves de Lima M,de Carvalho Junior LB,de Almeida SMV

    更新日期：2019-10-01 00:00:00

  • Structure determination of CAMP factor of Mobiluncus curtisii and insights into structural dynamics.

    abstract：:Bacterial vaginosis (BV) is a common type of vaginal inflammation caused by a proliferation of pathogenic bacteria, among which Mobiluncus curtisii. In our previous studies on M. curtisii genome, we identified the presence of a genomic fragment encoding a 25 kDa pore-forming toxin, the CAMP factor, which is known to b...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.10.107

    authors： Zeng W,Ma H,Fan W,Yang Y,Zhang C,Arnaud Kombe Kombe J,Fan X,Zhang Y,Dong Z,Shen Z,Zhou Y,Yang M,Jin T

    更新日期：2020-05-01 00:00:00

  • Effect of clay content on morphology and processability of electrospun keratin/poly(lactic acid) nanofiber.

    abstract：:This research work has concerned the development of volatile organic compounds (VOCs) removal filters from biomaterials, based on keratin extracted from chicken feather waste and poly(lactic acid) (PLA) (50/50%w/w) blend. Clay (Na-montmorillonite) was also added to the blend solution prior to carrying out an electro-s...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.01.041

    authors： Isarankura Na Ayutthaya S,Tanpichai S,Sangkhun W,Wootthikanokkhan J

    更新日期：2016-04-01 00:00:00

  • Preparation, characterization and wound healing effect of vaccarin-chitosan nanoparticles.

    abstract：:Wound healing is a complex, dynamic and difficult process. Much effort and attempt has been made to accelerate this process. The purpose of this study is to prepare nanoparticles loaded with vaccarin (VAC-NPS)hydrogel and evaluate its effect on promoting wound healing. In the present study, the physicochemical propert...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.10.182

    authors： Hou B,Qi M,Sun J,Ai M,Ma X,Cai W,Zhou Y,Ni L,Hu J,Xu F,Qiu L

    更新日期：2020-12-15 00:00:00

  • The solvent side of proteinaceous membrane-less organelles in light of aqueous two-phase systems.

    abstract：:Water represents a common denominator for liquid-liquid phase transitions leading to the formation of the polymer-based aqueous two-phase systems (ATPSs) and a set of the proteinaceous membrane-less organelles (PMLOs). ATPSs have a broad range of biotechnological applications, whereas PMLOs play a number of crucial ro...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2018.06.030

    authors： Zaslavsky BY,Ferreira LA,Darling AL,Uversky VN

    更新日期：2018-10-01 00:00:00

  • Injectable angiogenic and osteogenic carrageenan nanocomposite hydrogel for bone tissue engineering.

    abstract：:Functional biomaterials that couple angiogenesis and osteogenesis processes are vital for bone tissue engineering and bone remodeling. Herein we developed an injectable carrageenan nanocomposite hydrogel incorporated with whitlockite nanoparticles and an angiogenic drug, dimethyloxallylglycine. Synthesized whitlockite...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.10.182

    authors： Yegappan R,Selvaprithiviraj V,Amirthalingam S,Mohandas A,Hwang NS,Jayakumar R

    更新日期：2019-02-01 00:00:00

  • Isolation and identification of a sodium channel-inhibiting protein from eggs of black widow spiders.

    abstract：:The eggs of black widow spider (L. tredecimguttatus) have been demonstrated to be rich in biologically active components that exhibit great research value and application foreground. In the present study, a protein toxin, named Latroeggtoxin-II, was isolated from the eggs using the combination of gel filtration, ion e...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.01.004

    authors： Li J,Yan Y,Yu H,Peng X,Zhang Y,Hu W,Duan Z,Wang X,Liang S

    更新日期：2014-04-01 00:00:00

  • Partial purification of a Bacillus licheniformis levansucrase producing levan with antitumor activity.

    abstract：:The extracellular fructosyltransferase (FTase) of a novel strain of Bacillus licheniformis capable of producing fructooligosaccharides (FOS) and a polysaccharide type levan was obtained and partially purified. The purification was achieved by ammonium sulfate precipitation, DEAE cellulose and gel filtration chromatogr...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2012.04.030

    authors： Dahech I,Belghith KS,Belghith H,Mejdoub H

    更新日期：2012-10-01 00:00:00

  • Anticancer effect of atorvastatin nanostructured polymeric micelles based on stearyl-grafted chitosan.

    abstract：:The purpose of this study was to develop a new therapeutic approach for atorvastatin (ATV) adopting nanostructured polymeric micelles for its controlled delivery to the cancer cells. Amphiphilic block copolymers of stearyl chitosan (SC) and sulfated stearyl chitosan (S-SC) that could self assemble to form polymeric mi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2012.05.026

    authors： Mekhail GM,Kamel AO,Awad GA,Mortada ND

    更新日期：2012-11-01 00:00:00

  • Interfacial properties of ultrahigh methoxylated pectin.

    abstract：:The interfacial properties of ultrahigh methoxylated pectin (UHMP) prepared via esterification of citrus pectin (CP) were investigated. The intrinsic viscosity ([η]) of pectin was significantly decreased from 1211.5 mL/g to 294.9 mL/g as the degree of methylation (DM) increased from 63.18 ± 0.08% to 91.52 ± 0.11%. Sur...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.02.264

    authors： Liu J,Tan J,Hua X,Jiang Z,Wang M,Yang R,Cao Y

    更新日期：2020-06-01 00:00:00

  • Effect of O-glycosylation on amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil.

    abstract：:Glycosylation is one of the major post-translational modifications in eukaryotic cells and has been reported to affect the amyloid fibril formation in several amyloidogenic proteins and peptides. In this study, we expressed a Vλ6 light chain mutant, Wil, which is an amyloidogenic mutant in AL amyloidosis, by the yeast...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.10.194

    authors： Abe Y,Shibata H,Oyama K,Ueda T

    更新日期：2021-01-01 00:00:00

  • Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L.) Merr.].

    abstract：:Black soybeans are commonly consumed as health foods and used in traditional Chinese medicine, but they are rarely cultivated as edible sprouts. During germination, the composition of seeds undergoes distinct changes that cause variations in bioactivities. In this study, the water-soluble black soybean polysaccharide ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.02.145

    authors： Lee AL,Yu YP,Hsieh JF,Kuo MI,Ma YS,Lu CP

    更新日期：2018-07-01 00:00:00

  • Chitosan nanocomposite films based on Ag-NP and Au-NP biosynthesis by Bacillus Subtilis as packaging materials.

    abstract：:Chitosan-silver (CS-Ag) and Chitosan-gold (CS-Au) nanocomposites films were synthesized by a simple chemical method. A local bacterial isolate identified as Bacillus subtilis ss subtilis was found to be capable to synthesize both silver nanoparticles (Ag-NP) and gold nanoparticles (Au-NP) from silver nitrate (AgNO3) a...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.05.047

    authors： Youssef AM,Abdel-Aziz MS,El-Sayed SM

    更新日期：2014-08-01 00:00:00

  • Anti-glioma properties of DVL, a lectin purified from Dioclea violacea.

    abstract：:Plant lectins have been studied owing to their structural properties and biological effects that include agglutinating activity, antidepressant-like effect and antitumor property. The results from this work showed the effects of the lectin extracted from the Dioclea violacea plant (DVL) on the C6 rat glioma cell line....

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.08.106

    authors： Nascimento APM,Knaut JL,Rieger DK,Wolin IAV,Heinrich IA,Mann J,Juarez AV,Sosa LDV,De Paul AL,Moreira CG,Silva IB,Nobre CS,Osterne VJS,Nascimento KS,Cavada BS,Leal RB

    更新日期：2018-12-01 00:00:00

  • Synthesis and characterization of stable and biological active chitin-based polyurethane elastomers.

    abstract：:In this work, the preparation of novel biocompatible polyurethane (PU) elastomers were carried out using curcumin and 1,4-butanediol (1,4-BDO) via step growth polymerization reaction of hydroxyl terminated polybutadiene (HTPB), toluene diisocyanate (TDI) and chitin to improve the biocompatibility, antibacterial and an...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.11.097

    authors： Amjed N,Bhatti IA,Zia KM,Iqbal J,Jamil Y

    更新日期：2020-07-01 00:00:00