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Isolation and identification of a sodium channel-inhibiting protein from eggs of black widow spiders.

Abstract：

:The eggs of black widow spider (L. tredecimguttatus) have been demonstrated to be rich in biologically active components that exhibit great research value and application foreground. In the present study, a protein toxin, named Latroeggtoxin-II, was isolated from the eggs using the combination of gel filtration, ion exchange chromatography and reversed-phase high performance liquid chromatography. Electrospray mass spectrometric analysis indicated that the molecular weight of the protein was 28.69 kDa, and Edman degradation revealed that its N-terminal sequence was ESIQT STYVP NTPNQ KFDYE VGKDY-. After being abdominally injected into mice and P. americana, the protein could make the animals especially P. americana display a series of poisoning symptoms. Electrophysiological experiments demonstrated that the protein could selectively inhibit tetrodotoxin-resistant Na(+) channel currents in rat dorsal root ganglion neurons, without significant effect on the tetrodotoxin-sensitive Na(+) channel currents. Using multiple proteomic strategies, the purified protein was shown to have only a few similarities to the existing proteins in the databases, suggesting that it was a novel protein isolated from the eggs of black widow spiders.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Li J,Yan Y,Yu H,Peng X,Zhang Y,Hu W,Duan Z,Wang X,Liang S

doi

10.1016/j.ijbiomac.2014.01.004

subject

Has Abstract

pub_date

2014-04-01 00:00:00

pages

115-20

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(14)00005-1

journal_volume

65

pub_type

杂志文章

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