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Characterization by mass spectrometry of poly(3-hydroxyalkanoates) produced by Rhodospirillum rubrum from 3-hydroxyacids.

Abstract：

:The sequence distributions of two microbial copolyesters obtained by fermentation of Rhodospirillum rubrum, grown with 3-hydroxyhexanoic or 3-hydroxyheptanoic acids, were determined by analyzing the oligomers prepared by partial pyrolysis or partial methanolysis of these copolyesters using fast atom bombardment mass spectrometry (FAB-MS). Oligomers up to pentamers were identified in the case of partial pyrolysis and up to tetradecamers in the case of partial methanolysis. The comparison between the experimental and calculated peak intensities of FAB mass spectra allows the calculation of compositions and sequence distributions, which in these copolyesters follow Bernoullian statistics, indicating that they are random terpolyesters.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Ballistreri A,Giuffrida M,Impallomeni G,Lenz RW,Fuller RC

doi

10.1016/s0141-8130(99)00085-9

keywords:

subject

Has Abstract

pub_date

1999-11-01 00:00:00

pages

201-11

issue

2-3

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(99)00085-9

journal_volume

26

pub_type

杂志文章

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